Rhizobium phage V1VFA-S

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G7WX15|A0A7G7WX15_9CAUD Glutaredoxin domain-containing protein OS=Rhizobium phage V1VFA-S OX=2763532 GN=V1VFAS_134 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.26CFYY5 pKa = 10.35DD6 pKa = 4.06EE7 pKa = 4.94PGEE10 pKa = 3.97PDD12 pKa = 3.7YY13 pKa = 11.7LGLEE17 pKa = 3.94AAKK20 pKa = 10.51AQEE23 pKa = 4.0ALEE26 pKa = 4.77ACDD29 pKa = 4.15EE30 pKa = 4.55MYY32 pKa = 11.19LHH34 pKa = 6.92VYY36 pKa = 10.32DD37 pKa = 4.87GAGLMGWVFVVNDD50 pKa = 3.8ADD52 pKa = 4.28GDD54 pKa = 4.04PEE56 pKa = 4.14EE57 pKa = 5.1QINDD61 pKa = 3.46YY62 pKa = 10.72AGNRR66 pKa = 11.84ITEE69 pKa = 3.86ILRR72 pKa = 11.84FEE74 pKa = 4.32EE75 pKa = 4.33AA76 pKa = 3.8

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G7WX05|A0A7G7WX05_9CAUD Uncharacterized protein OS=Rhizobium phage V1VFA-S OX=2763532 GN=V1VFAS_101 PE=4 SV=1
MM1 pKa = 7.37SVQKK5 pKa = 10.38RR6 pKa = 11.84KK7 pKa = 10.17RR8 pKa = 11.84EE9 pKa = 3.95LLQLARR15 pKa = 11.84AICPKK20 pKa = 9.96ATIEE24 pKa = 4.26HH25 pKa = 6.63SGHH28 pKa = 6.51HH29 pKa = 5.33LQITIFGPSGSRR41 pKa = 11.84KK42 pKa = 9.29VFCASTAGDD51 pKa = 3.49HH52 pKa = 7.49RR53 pKa = 11.84DD54 pKa = 3.45TKK56 pKa = 10.57NVKK59 pKa = 9.82RR60 pKa = 11.84DD61 pKa = 3.37LLAAARR67 pKa = 11.84AIGLIANQNEE77 pKa = 4.24PPASQRR83 pKa = 3.35

Molecular weight: 9.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

157

0

157

35426

37

2064

225.6

24.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.823 ± 0.315

0.793 ± 0.066

6.45 ± 0.178

6.964 ± 0.303

3.853 ± 0.133

7.396 ± 0.272

1.919 ± 0.12

5.699 ± 0.115

6.055 ± 0.268

7.957 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.151

3.907 ± 0.192

4.138 ± 0.165

3.557 ± 0.175

5.758 ± 0.185

5.197 ± 0.187

6.027 ± 0.353

6.631 ± 0.196

1.313 ± 0.08

2.848 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski