Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Cellvibrionaceae; Saccharophagus; Saccharophagus degradans

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3999 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q21PR3|Q21PR3_SACD2 S-(hydroxymethyl)glutathione dehydrogenase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) OX=203122 GN=Sde_0052 PE=3 SV=1
MM1 pKa = 6.23QHH3 pKa = 6.58HH4 pKa = 6.79YY5 pKa = 11.2KK6 pKa = 10.75LILPLTIAALASACGSGGDD25 pKa = 4.87DD26 pKa = 4.55GGDD29 pKa = 3.37SSGGSSSKK37 pKa = 9.99YY38 pKa = 10.3VYY40 pKa = 9.69MSTYY44 pKa = 9.09SSEE47 pKa = 3.81VGRR50 pKa = 11.84FVDD53 pKa = 3.75SAVGGVEE60 pKa = 3.87YY61 pKa = 10.76CQVLFNVRR69 pKa = 11.84NLDD72 pKa = 3.59DD73 pKa = 5.62SIDD76 pKa = 3.6AEE78 pKa = 5.33RR79 pKa = 11.84IDD81 pKa = 3.46QCGITDD87 pKa = 3.49SDD89 pKa = 3.92GSFSYY94 pKa = 9.92TLSVQGTEE102 pKa = 4.63DD103 pKa = 4.61LINPVLDD110 pKa = 3.74GDD112 pKa = 4.35GLQGRR117 pKa = 11.84QSAIIDD123 pKa = 3.34SAIRR127 pKa = 11.84FNVRR131 pKa = 11.84GIQLPDD137 pKa = 3.35VPVDD141 pKa = 3.6QLITPLSFSADD152 pKa = 3.14NSLDD156 pKa = 3.29QTAINVARR164 pKa = 11.84LLVSLDD170 pKa = 3.61SNNNLEE176 pKa = 4.27DD177 pKa = 4.61GIYY180 pKa = 10.16ISGDD184 pKa = 3.31DD185 pKa = 3.89FSNNILDD192 pKa = 4.44FGADD196 pKa = 3.33SEE198 pKa = 4.9VFATAAQVTLNEE210 pKa = 4.38AEE212 pKa = 4.18LTLAAEE218 pKa = 4.85DD219 pKa = 3.9EE220 pKa = 5.03VITHH224 pKa = 5.45VNEE227 pKa = 4.18SLSNLTQLAGTWVFANNSQQSFVQVSLFSDD257 pKa = 4.04GTYY260 pKa = 8.86THH262 pKa = 7.05IEE264 pKa = 4.1KK265 pKa = 10.58GDD267 pKa = 3.97EE268 pKa = 3.9IDD270 pKa = 3.59PSGMEE275 pKa = 3.74WGSYY279 pKa = 6.6TLNNGEE285 pKa = 4.36ITFKK289 pKa = 10.91AYY291 pKa = 9.56TDD293 pKa = 3.51NNGAIGLIDD302 pKa = 4.11SPLGSTLTTPTTLGYY317 pKa = 10.6SLSEE321 pKa = 4.22DD322 pKa = 3.99TNTLTLVDD330 pKa = 4.19GNEE333 pKa = 4.14SFDD336 pKa = 3.89LVRR339 pKa = 11.84AANITDD345 pKa = 4.23SIVGTWKK352 pKa = 10.23PKK354 pKa = 9.09YY355 pKa = 8.81KK356 pKa = 9.89TRR358 pKa = 11.84AYY360 pKa = 10.05YY361 pKa = 9.85ISLSFFEE368 pKa = 4.63NGLYY372 pKa = 10.29SHH374 pKa = 7.1GEE376 pKa = 3.82LGDD379 pKa = 3.7ADD381 pKa = 4.23EE382 pKa = 5.44PDD384 pKa = 4.57GIEE387 pKa = 4.16IGQYY391 pKa = 10.69DD392 pKa = 4.68LNSSTGRR399 pKa = 11.84LIPTLYY405 pKa = 10.78VDD407 pKa = 4.13TNGSTGLSDD416 pKa = 3.58AVNEE420 pKa = 4.5GEE422 pKa = 4.33VLTVEE427 pKa = 4.32VNGDD431 pKa = 3.32EE432 pKa = 4.19LTFNGEE438 pKa = 4.25MVFVRR443 pKa = 11.84QQ444 pKa = 3.74

Molecular weight: 47.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q21DP3|Q21DP3_SACD2 Uncharacterized protein OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) OX=203122 GN=Sde_3931 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.91VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.44GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3999

0

3999

1456122

38

14609

364.1

40.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.549 ± 0.04

1.028 ± 0.016

5.584 ± 0.048

6.098 ± 0.043

4.032 ± 0.029

7.085 ± 0.037

2.102 ± 0.023

5.95 ± 0.032

4.877 ± 0.055

9.816 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.026

4.728 ± 0.039

4.091 ± 0.026

4.135 ± 0.031

4.448 ± 0.041

6.897 ± 0.063

5.74 ± 0.048

7.105 ± 0.042

1.358 ± 0.019

3.205 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski