Clostridium sp. CAG:451

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7KEG2|R7KEG2_9CLOT Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B OS=Clostridium sp. CAG:451 OX=1262809 GN=gatB PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.37PIYY6 pKa = 8.29ATPLSEE12 pKa = 4.33TNTTYY17 pKa = 10.85AYY19 pKa = 10.12KK20 pKa = 9.63LTIGGKK26 pKa = 10.09DD27 pKa = 3.29GIIANEE33 pKa = 3.81SMIGYY38 pKa = 9.53FYY40 pKa = 10.88EE41 pKa = 4.39FTNMTSIDD49 pKa = 4.3LSVLDD54 pKa = 3.98TSEE57 pKa = 4.07VTNMSNMFSACISLTSLDD75 pKa = 3.47VSNFDD80 pKa = 3.38TSKK83 pKa = 11.09VIDD86 pKa = 3.9MSDD89 pKa = 3.37MFDD92 pKa = 2.94QCYY95 pKa = 9.7MLTSLDD101 pKa = 3.44LRR103 pKa = 11.84NFNTSEE109 pKa = 4.21VIDD112 pKa = 3.91MSSMFSACISLTSLDD127 pKa = 3.47VSNFDD132 pKa = 3.32TSKK135 pKa = 10.6VINMSDD141 pKa = 3.26MFSEE145 pKa = 4.75CSSLTSLDD153 pKa = 3.57LKK155 pKa = 10.6EE156 pKa = 4.46SRR158 pKa = 11.84FF159 pKa = 3.79

Molecular weight: 17.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7KB23|R7KB23_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:451 OX=1262809 GN=BN663_00461 PE=4 SV=1
MM1 pKa = 7.83AIKK4 pKa = 10.31KK5 pKa = 8.03FRR7 pKa = 11.84PTTPGQRR14 pKa = 11.84NKK16 pKa = 9.66STLVNEE22 pKa = 5.47EE23 pKa = 3.96ITKK26 pKa = 8.26STPEE30 pKa = 3.66KK31 pKa = 10.8SLVVTLNKK39 pKa = 10.09KK40 pKa = 10.19AGRR43 pKa = 11.84NNQGKK48 pKa = 8.01ITVRR52 pKa = 11.84HH53 pKa = 5.14QGGGVKK59 pKa = 9.9RR60 pKa = 11.84KK61 pKa = 9.91YY62 pKa = 10.45RR63 pKa = 11.84IIDD66 pKa = 3.65FKK68 pKa = 11.18RR69 pKa = 11.84NKK71 pKa = 9.76FDD73 pKa = 3.35VEE75 pKa = 4.35GVVASIEE82 pKa = 4.01YY83 pKa = 10.59DD84 pKa = 3.46PNRR87 pKa = 11.84SANIALINYY96 pKa = 9.36LDD98 pKa = 3.67GEE100 pKa = 4.28KK101 pKa = 10.22RR102 pKa = 11.84YY103 pKa = 10.24IIAPKK108 pKa = 9.58GLKK111 pKa = 10.45VGDD114 pKa = 4.34KK115 pKa = 10.39IVAGSNADD123 pKa = 3.12IKK125 pKa = 10.11TGNALPIMNIPVGTVIHH142 pKa = 6.49NIEE145 pKa = 4.22LRR147 pKa = 11.84PGKK150 pKa = 10.2GGSLARR156 pKa = 11.84SAGSSAQILGRR167 pKa = 11.84EE168 pKa = 4.35GNYY171 pKa = 10.43VMIRR175 pKa = 11.84LSSGEE180 pKa = 3.73QRR182 pKa = 11.84RR183 pKa = 11.84VLGTCMATIGVVGNEE198 pKa = 4.07DD199 pKa = 3.66SNLVKK204 pKa = 10.51LGKK207 pKa = 10.1AGRR210 pKa = 11.84KK211 pKa = 6.0RR212 pKa = 11.84HH213 pKa = 5.13MGIRR217 pKa = 11.84PTVRR221 pKa = 11.84GSVMNPNDD229 pKa = 3.69HH230 pKa = 6.5PHH232 pKa = 6.52GGGEE236 pKa = 3.83GRR238 pKa = 11.84APIGRR243 pKa = 11.84KK244 pKa = 10.0GPVTPWGKK252 pKa = 9.06PALGYY257 pKa = 7.87KK258 pKa = 8.51TRR260 pKa = 11.84NNKK263 pKa = 8.52RR264 pKa = 11.84TDD266 pKa = 2.87KK267 pKa = 10.95FIVRR271 pKa = 11.84RR272 pKa = 11.84RR273 pKa = 11.84GSKK276 pKa = 9.95

Molecular weight: 29.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1322

0

1322

387124

29

2365

292.8

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.463 ± 0.057

1.021 ± 0.024

6.254 ± 0.052

7.207 ± 0.081

4.308 ± 0.054

5.218 ± 0.069

1.335 ± 0.026

9.131 ± 0.069

9.961 ± 0.082

9.659 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.031

6.838 ± 0.072

2.449 ± 0.035

2.214 ± 0.037

3.29 ± 0.047

6.627 ± 0.066

5.836 ± 0.077

6.092 ± 0.052

0.528 ± 0.015

5.157 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski