Acinetobacter phage vB_AbaM_ME3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Metrivirus; Acinetobacter virus ME3

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 326 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172Q054|A0A172Q054_9CAUD Putative HNH endonuclease protein OS=Acinetobacter phage vB_AbaM_ME3 OX=1837876 GN=ME3_63 PE=4 SV=1
MM1 pKa = 7.65NDD3 pKa = 3.16SEE5 pKa = 5.36VYY7 pKa = 8.19TFKK10 pKa = 10.77VRR12 pKa = 11.84YY13 pKa = 8.95YY14 pKa = 10.78LDD16 pKa = 4.07RR17 pKa = 11.84DD18 pKa = 4.17SIQEE22 pKa = 4.09LNSLSHH28 pKa = 6.55NEE30 pKa = 4.66LMDD33 pKa = 3.55ILQRR37 pKa = 11.84LYY39 pKa = 10.17ISSDD43 pKa = 2.99KK44 pKa = 10.81EE45 pKa = 4.01YY46 pKa = 11.62VEE48 pKa = 4.22ITLSLSSSGGVNILFVDD65 pKa = 4.9DD66 pKa = 4.13YY67 pKa = 11.14PIVKK71 pKa = 9.75NQFITLYY78 pKa = 9.53NGEE81 pKa = 4.23VTDD84 pKa = 3.94EE85 pKa = 4.19SEE87 pKa = 4.25YY88 pKa = 11.62

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172Q064|A0A172Q064_9CAUD Putative exodeoxyribonuclease OS=Acinetobacter phage vB_AbaM_ME3 OX=1837876 GN=ME3_85 PE=4 SV=1
MM1 pKa = 7.98IDD3 pKa = 3.14NQGPIKK9 pKa = 9.9RR10 pKa = 11.84DD11 pKa = 3.04RR12 pKa = 11.84VQKK15 pKa = 10.28YY16 pKa = 8.47GRR18 pKa = 11.84KK19 pKa = 9.55SGLQEE24 pKa = 3.79AKK26 pKa = 10.6SFAEE30 pKa = 4.02RR31 pKa = 11.84NGMKK35 pKa = 9.52TLPLLTNKK43 pKa = 9.79HH44 pKa = 5.44KK45 pKa = 11.23NKK47 pKa = 10.31LYY49 pKa = 10.38RR50 pKa = 11.84KK51 pKa = 9.44NIEE54 pKa = 4.03RR55 pKa = 11.84NAKK58 pKa = 9.39SSSRR62 pKa = 11.84VIPPEE67 pKa = 3.42EE68 pKa = 3.96RR69 pKa = 11.84KK70 pKa = 10.09FKK72 pKa = 10.93LSTITGIGNQTSSVATPFLPSSRR95 pKa = 11.84IMNPPQPAKK104 pKa = 10.39SKK106 pKa = 8.41YY107 pKa = 8.26TSFLGDD113 pKa = 3.73MYY115 pKa = 11.65NKK117 pKa = 10.25LLSSFDD123 pKa = 3.48KK124 pKa = 10.88STDD127 pKa = 3.44QAIGGALGFTNRR139 pKa = 11.84NVVPGFTQNTSSGLSRR155 pKa = 11.84FDD157 pKa = 3.73VGNGDD162 pKa = 3.51PFKK165 pKa = 10.88YY166 pKa = 10.37SNRR169 pKa = 11.84FMKK172 pKa = 10.71GDD174 pKa = 4.08LDD176 pKa = 4.16DD177 pKa = 5.72LRR179 pKa = 11.84AAYY182 pKa = 9.61INEE185 pKa = 3.81AAKK188 pKa = 10.2GVRR191 pKa = 11.84VRR193 pKa = 11.84GDD195 pKa = 3.22NVNPFDD201 pKa = 3.87NSRR204 pKa = 11.84GARR207 pKa = 11.84NLGRR211 pKa = 11.84LRR213 pKa = 11.84QLQNRR218 pKa = 11.84INGGGQADD226 pKa = 5.67LIRR229 pKa = 11.84EE230 pKa = 4.37SNSIRR235 pKa = 11.84SQLDD239 pKa = 3.36DD240 pKa = 3.27VKK242 pKa = 11.01GRR244 pKa = 11.84QKK246 pKa = 11.06SIFPPRR252 pKa = 11.84NIGQGQSNYY261 pKa = 9.84EE262 pKa = 3.98SLVRR266 pKa = 11.84QQEE269 pKa = 4.39SLEE272 pKa = 3.87ARR274 pKa = 11.84QVVVNRR280 pKa = 11.84KK281 pKa = 8.6LEE283 pKa = 4.15RR284 pKa = 11.84NARR287 pKa = 11.84ILSTANEE294 pKa = 4.33YY295 pKa = 11.04SGATHH300 pKa = 7.12IKK302 pKa = 10.28GFLPKK307 pKa = 10.07EE308 pKa = 4.09GVGGYY313 pKa = 10.67GLGVSNYY320 pKa = 9.11LAASRR325 pKa = 11.84KK326 pKa = 8.1EE327 pKa = 3.85LFVRR331 pKa = 11.84GLGFTGGILGPSGAEE346 pKa = 3.24ALMNSFGIVTAKK358 pKa = 9.77QRR360 pKa = 11.84AVTQSKK366 pKa = 10.41GLLGKK371 pKa = 9.25MFSAGFQAPLMTYY384 pKa = 10.82GSLLFTASSGGDD396 pKa = 2.91LSDD399 pKa = 4.21FVEE402 pKa = 4.52TQLTYY407 pKa = 11.09AGGLQGWRR415 pKa = 11.84IGSSVGGMLTKK426 pKa = 10.14GTGANLSQIKK436 pKa = 8.97GGNMISAMRR445 pKa = 11.84AQGGRR450 pKa = 11.84AVLRR454 pKa = 11.84GAAGVVGGVTGFALGAGLVAGASWLAQDD482 pKa = 3.82ISSNRR487 pKa = 11.84STIRR491 pKa = 11.84KK492 pKa = 7.29IAKK495 pKa = 10.1DD496 pKa = 3.73FTTKK500 pKa = 9.41TATMNTQNTRR510 pKa = 11.84QSLTMRR516 pKa = 11.84QMAISKK522 pKa = 9.32LAKK525 pKa = 10.08SGLNDD530 pKa = 3.73RR531 pKa = 11.84ATLLGNEE538 pKa = 3.99SRR540 pKa = 11.84VLKK543 pKa = 10.97GLMM546 pKa = 3.76

Molecular weight: 58.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

326

0

326

73381

27

5419

225.1

25.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.301 ± 0.31

1.055 ± 0.09

5.997 ± 0.106

6.119 ± 0.218

4.467 ± 0.121

5.024 ± 0.201

1.804 ± 0.106

7.675 ± 0.151

7.203 ± 0.211

9.212 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.906 ± 0.08

6.517 ± 0.141

3.258 ± 0.074

3.355 ± 0.073

3.624 ± 0.101

8.246 ± 0.15

7.069 ± 0.35

6.485 ± 0.105

0.921 ± 0.052

4.76 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski