Dishui Lake virophage 8

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XNK5|A0A6G6XNK5_9VIRU Putative collagen triple helix repeat-containing protein OS=Dishui Lake virophage 8 OX=2704069 PE=4 SV=1
MM1 pKa = 7.24SVSSIINPTDD11 pKa = 3.1GKK13 pKa = 10.73IYY15 pKa = 10.1TEE17 pKa = 4.89LYY19 pKa = 10.34NGGGGYY25 pKa = 10.37NLGEE29 pKa = 3.98VLSFGNNASNPTTGLPQDD47 pKa = 3.44ATDD50 pKa = 4.79FNNLGCISIEE60 pKa = 4.04TGKK63 pKa = 10.73VYY65 pKa = 10.54QGNNLDD71 pKa = 4.28LEE73 pKa = 4.49IGEE76 pKa = 5.19AGDD79 pKa = 3.42TLKK82 pKa = 10.75IIGAITKK89 pKa = 10.4GSILVGNGVEE99 pKa = 4.47TKK101 pKa = 9.82EE102 pKa = 4.51LPVGANGLVLKK113 pKa = 10.48ANSAATYY120 pKa = 8.56GVEE123 pKa = 3.88WAVDD127 pKa = 3.6GTTGITGVSAGTNIGIDD144 pKa = 3.46NTNPIVPIINFATPTTSNISLGVGTFIEE172 pKa = 4.73AKK174 pKa = 10.39DD175 pKa = 3.78NYY177 pKa = 8.94STPNFAMTLDD187 pKa = 3.36ATGFNDD193 pKa = 3.93TYY195 pKa = 11.35TNGAVEE201 pKa = 4.33NKK203 pKa = 10.2EE204 pKa = 4.21DD205 pKa = 3.72IEE207 pKa = 4.62VNATSVVQLLSATDD221 pKa = 3.37GGSYY225 pKa = 10.98LNTDD229 pKa = 3.5TTNVSNTGIVEE240 pKa = 4.11NLSATNLSNNNIGNVSFTCYY260 pKa = 10.51SNSAGLACGCSAPTTPPYY278 pKa = 10.56PEE280 pKa = 4.09VSATAGLSATTTNAQLTISQSAPFAISYY308 pKa = 7.62STILDD313 pKa = 3.33INGINQNNTGGVAGFTINTNTQPLALTTGDD343 pKa = 4.42NITFSADD350 pKa = 3.85NIDD353 pKa = 3.92LDD355 pKa = 3.72ATGRR359 pKa = 11.84LILPSLASGDD369 pKa = 3.84YY370 pKa = 10.74LDD372 pKa = 5.15YY373 pKa = 11.34NAGSLKK379 pKa = 10.2IVNDD383 pKa = 3.85SVAGSANPLLVLQNNNNTAGATTFEE408 pKa = 4.78TYY410 pKa = 10.85KK411 pKa = 10.65NDD413 pKa = 3.34QPTSTGGDD421 pKa = 3.72NIASWSATCNTNVGKK436 pKa = 8.65TEE438 pKa = 3.64ISRR441 pKa = 11.84INHH444 pKa = 5.69IALLTEE450 pKa = 4.33LVLLIMTAVLPLLVRR465 pKa = 11.84LIRR468 pKa = 11.84QQ469 pKa = 3.25

Molecular weight: 48.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XMW4|A0A6G6XMW4_9VIRU Putative major capsid protein OS=Dishui Lake virophage 8 OX=2704069 PE=4 SV=1
MM1 pKa = 7.28NVFNHH6 pKa = 7.17FIYY9 pKa = 10.2IIYY12 pKa = 8.94IDD14 pKa = 4.38KK15 pKa = 10.35MFSNQLKK22 pKa = 9.43EE23 pKa = 3.93LKK25 pKa = 10.32IPIKK29 pKa = 10.06TYY31 pKa = 11.18LNTAKK36 pKa = 10.02QRR38 pKa = 11.84AKK40 pKa = 10.68NAGYY44 pKa = 10.1DD45 pKa = 3.7PKK47 pKa = 10.99LLSLSKK53 pKa = 10.9DD54 pKa = 3.07KK55 pKa = 10.73EE56 pKa = 4.14YY57 pKa = 11.25KK58 pKa = 10.44LNYY61 pKa = 9.8DD62 pKa = 3.35GVNFGRR68 pKa = 11.84TGYY71 pKa = 10.95GDD73 pKa = 4.09FIIWSILEE81 pKa = 3.88DD82 pKa = 3.29RR83 pKa = 11.84GLVEE87 pKa = 5.62KK88 pKa = 10.87GYY90 pKa = 11.25ADD92 pKa = 3.23KK93 pKa = 10.84KK94 pKa = 9.79QNVFRR99 pKa = 11.84ASHH102 pKa = 5.83SKK104 pKa = 10.47IKK106 pKa = 10.71GDD108 pKa = 3.23WKK110 pKa = 11.11NNPKK114 pKa = 10.34SPNNLALKK122 pKa = 10.4INWW125 pKa = 3.44

Molecular weight: 14.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

6793

125

890

424.6

47.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.668 ± 0.681

0.972 ± 0.18

6.198 ± 0.295

5.491 ± 0.701

3.842 ± 0.293

7.272 ± 1.098

1.104 ± 0.203

7.169 ± 0.474

8.008 ± 1.261

8.052 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.305

7.316 ± 0.659

4.887 ± 0.432

4.431 ± 0.687

3.562 ± 0.489

6.58 ± 0.658

6.242 ± 0.725

5.182 ± 0.334

1.075 ± 0.145

4.608 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski