Mycobacterium phage Timshel

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; Mycobacterium virus Timshel

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1DB72|G1DB72_9CAUD DNA primase OS=Mycobacterium phage Timshel OX=1032895 GN=54 PE=4 SV=1
MM1 pKa = 7.56SYY3 pKa = 8.63KK4 pKa = 10.04TGDD7 pKa = 3.55VAVVTGPLRR16 pKa = 11.84LASGSPWVYY25 pKa = 9.67FAEE28 pKa = 4.66GDD30 pKa = 3.68VVDD33 pKa = 4.21IVRR36 pKa = 11.84GEE38 pKa = 4.34DD39 pKa = 3.31EE40 pKa = 5.38DD41 pKa = 6.21GDD43 pKa = 4.05VLAVRR48 pKa = 11.84RR49 pKa = 11.84SDD51 pKa = 5.0GLDD54 pKa = 3.04QYY56 pKa = 11.48IVGTSLTPFSTVAEE70 pKa = 4.48DD71 pKa = 5.24PEE73 pKa = 4.89IDD75 pKa = 2.92WGQVHH80 pKa = 7.52DD81 pKa = 4.13YY82 pKa = 10.71VYY84 pKa = 11.06QEE86 pKa = 4.71GDD88 pKa = 3.26DD89 pKa = 3.91DD90 pKa = 4.21AA91 pKa = 7.56

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1DB87|G1DB87_9CAUD Cas4 family exonuclease OS=Mycobacterium phage Timshel OX=1032895 GN=69 PE=4 SV=1
MM1 pKa = 7.45PAKK4 pKa = 10.53HH5 pKa = 7.02PDD7 pKa = 3.28QTHH10 pKa = 5.54TRR12 pKa = 11.84DD13 pKa = 3.16TSGWSNTPDD22 pKa = 3.58PNSPVLRR29 pKa = 11.84SPYY32 pKa = 10.25APHH35 pKa = 6.01EE36 pKa = 4.36TGAVLRR42 pKa = 11.84MQRR45 pKa = 11.84AGFSGLGISSTLRR58 pKa = 11.84MSPGVVMSKK67 pKa = 10.4LSQQMGEE74 pKa = 3.94EE75 pKa = 4.32GTAYY79 pKa = 10.12RR80 pKa = 11.84RR81 pKa = 11.84QRR83 pKa = 11.84PIHH86 pKa = 6.66DD87 pKa = 3.87ALIKK91 pKa = 10.58KK92 pKa = 7.79GTVV95 pKa = 2.84

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

16704

21

1122

196.5

21.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.86 ± 0.439

0.832 ± 0.121

6.436 ± 0.264

6.124 ± 0.301

3.335 ± 0.194

9.614 ± 0.64

1.976 ± 0.174

4.759 ± 0.158

4.31 ± 0.224

7.95 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.136

3.101 ± 0.159

5.627 ± 0.296

3.837 ± 0.246

6.232 ± 0.384

5.16 ± 0.161

6.196 ± 0.239

7.358 ± 0.183

2.053 ± 0.169

2.784 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski