Changjiang crawfish virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFS4|A0A1L3KFS4_9VIRU Uncharacterized protein OS=Changjiang crawfish virus 7 OX=1922771 PE=4 SV=1
MM1 pKa = 7.84PEE3 pKa = 3.8VSVDD7 pKa = 3.45QQGYY11 pKa = 7.01SQKK14 pKa = 10.5LPKK17 pKa = 10.07RR18 pKa = 11.84LFDD21 pKa = 3.57IRR23 pKa = 11.84GLPGRR28 pKa = 11.84GTLWTHH34 pKa = 6.43ANDD37 pKa = 3.28VANVVQSVHH46 pKa = 5.44EE47 pKa = 4.18RR48 pKa = 11.84VLGRR52 pKa = 11.84TTSKK56 pKa = 10.0GWEE59 pKa = 3.79RR60 pKa = 11.84TLLPEE65 pKa = 4.22EE66 pKa = 4.52GAFDD70 pKa = 3.64SEE72 pKa = 4.32GMVRR76 pKa = 11.84FSTRR80 pKa = 11.84LKK82 pKa = 10.47QKK84 pKa = 10.32LGSHH88 pKa = 6.26SLPVTEE94 pKa = 5.45QDD96 pKa = 3.4FLSHH100 pKa = 6.14YY101 pKa = 9.81RR102 pKa = 11.84GQKK105 pKa = 7.76RR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84YY109 pKa = 7.91EE110 pKa = 3.7AAVSSLRR117 pKa = 11.84AKK119 pKa = 9.86PVQRR123 pKa = 11.84ADD125 pKa = 3.38SYY127 pKa = 11.63PSVFLKK133 pKa = 10.66AEE135 pKa = 3.97KK136 pKa = 9.52WRR138 pKa = 11.84EE139 pKa = 3.96EE140 pKa = 3.95KK141 pKa = 10.32PGRR144 pKa = 11.84LISARR149 pKa = 11.84SPRR152 pKa = 11.84YY153 pKa = 7.9NVEE156 pKa = 3.54VGRR159 pKa = 11.84YY160 pKa = 8.15LLPLEE165 pKa = 4.31PLVYY169 pKa = 10.1QAIDD173 pKa = 4.12GVWGSATIMKK183 pKa = 10.2GYY185 pKa = 8.24TPEE188 pKa = 3.47RR189 pKa = 11.84RR190 pKa = 11.84AAVVRR195 pKa = 11.84GHH197 pKa = 6.85WDD199 pKa = 3.14SFGDD203 pKa = 3.97PVAVGHH209 pKa = 6.79DD210 pKa = 3.69FSKK213 pKa = 10.84FDD215 pKa = 3.2QHH217 pKa = 6.7ISKK220 pKa = 10.6RR221 pKa = 11.84ALQYY225 pKa = 10.07EE226 pKa = 4.13HH227 pKa = 6.69GVYY230 pKa = 10.36LRR232 pKa = 11.84AYY234 pKa = 10.05AGDD237 pKa = 3.49EE238 pKa = 4.08HH239 pKa = 6.77LQKK242 pKa = 10.93LLSWQLEE249 pKa = 4.57TTCYY253 pKa = 11.14ANVRR257 pKa = 11.84DD258 pKa = 4.02GRR260 pKa = 11.84VKK262 pKa = 9.57YY263 pKa = 7.51TVKK266 pKa = 10.23GGRR269 pKa = 11.84MSGDD273 pKa = 3.27MNTAMGNCIISAGLIWAYY291 pKa = 10.2AAEE294 pKa = 4.41SGVQLRR300 pKa = 11.84AVVDD304 pKa = 3.91GDD306 pKa = 3.82DD307 pKa = 3.43SVVFMEE313 pKa = 5.34KK314 pKa = 10.1KK315 pKa = 10.33DD316 pKa = 3.69LQRR319 pKa = 11.84YY320 pKa = 7.31LAGIEE325 pKa = 3.5GWMAKK330 pKa = 9.98RR331 pKa = 11.84GFRR334 pKa = 11.84LVTEE338 pKa = 4.27EE339 pKa = 3.99PVYY342 pKa = 10.42EE343 pKa = 4.03INRR346 pKa = 11.84VEE348 pKa = 4.3FCQCRR353 pKa = 11.84YY354 pKa = 9.22MDD356 pKa = 4.21TVPPTMVRR364 pKa = 11.84NPLKK368 pKa = 10.77AITQDD373 pKa = 3.48HH374 pKa = 6.24AWIEE378 pKa = 4.23DD379 pKa = 3.41RR380 pKa = 11.84SISYY384 pKa = 11.09AEE386 pKa = 3.91VLAATGLGGLSLYY399 pKa = 10.74GHH401 pKa = 6.79IPVLGAYY408 pKa = 9.76YY409 pKa = 10.7DD410 pKa = 3.77LLARR414 pKa = 11.84TTQPSRR420 pKa = 11.84RR421 pKa = 11.84TLNRR425 pKa = 11.84LDD427 pKa = 5.17FRR429 pKa = 11.84SSWLRR434 pKa = 11.84DD435 pKa = 3.04ATMSGGFTEE444 pKa = 4.66PSEE447 pKa = 3.92QARR450 pKa = 11.84YY451 pKa = 9.36QFWLTWGMSPGEE463 pKa = 4.08QRR465 pKa = 11.84AHH467 pKa = 5.49EE468 pKa = 4.27MNFRR472 pKa = 11.84ACDD475 pKa = 3.76LQDD478 pKa = 4.17LVASDD483 pKa = 5.2TITKK487 pKa = 8.4ATRR490 pKa = 11.84NEE492 pKa = 3.82KK493 pKa = 9.31TDD495 pKa = 3.64PYY497 pKa = 10.47GNNYY501 pKa = 9.37YY502 pKa = 10.77LL503 pKa = 4.42

Molecular weight: 57.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG56|A0A1L3KG56_9VIRU Uncharacterized protein OS=Changjiang crawfish virus 7 OX=1922771 PE=4 SV=1
MM1 pKa = 7.35VKK3 pKa = 9.67TKK5 pKa = 8.52GTKK8 pKa = 10.2GKK10 pKa = 10.05GKK12 pKa = 9.0SAKK15 pKa = 9.68RR16 pKa = 11.84VAKK19 pKa = 9.95RR20 pKa = 11.84PAMRR24 pKa = 11.84VRR26 pKa = 11.84GPEE29 pKa = 3.65GHH31 pKa = 6.27EE32 pKa = 3.68ARR34 pKa = 11.84LARR37 pKa = 11.84LIMDD41 pKa = 4.32PCGAEE46 pKa = 3.99LTTGYY51 pKa = 11.49ALATEE56 pKa = 4.63GLVQRR61 pKa = 11.84FNRR64 pKa = 11.84FITPVATTEE73 pKa = 4.15TNFAYY78 pKa = 9.73IFNPLSHH85 pKa = 6.73AAEE88 pKa = 4.61SIVQKK93 pKa = 10.93LSTGTGAATNISSTAPGEE111 pKa = 4.32AYY113 pKa = 10.84LDD115 pKa = 3.92ANADD119 pKa = 3.6AVSTVAACMQILYY132 pKa = 9.14TGKK135 pKa = 9.85LVDD138 pKa = 3.44RR139 pKa = 11.84KK140 pKa = 10.71GYY142 pKa = 10.2IGVCQAPWFVMNDD155 pKa = 2.89IATGTTDD162 pKa = 4.52LPTLLSYY169 pKa = 10.63CQAIQPVGSEE179 pKa = 3.8TLEE182 pKa = 3.97IKK184 pKa = 10.72FSPTIRR190 pKa = 11.84SLLGQTANVEE200 pKa = 4.27TSGGIDD206 pKa = 3.21NVLMVVAIGVDD217 pKa = 3.44PNQFVVKK224 pKa = 8.29FTSVYY229 pKa = 9.9EE230 pKa = 4.01YY231 pKa = 11.03VPKK234 pKa = 10.42FALGAPAPRR243 pKa = 11.84ATKK246 pKa = 9.75TYY248 pKa = 10.45IPGAPEE254 pKa = 4.65RR255 pKa = 11.84IVSTLDD261 pKa = 3.37RR262 pKa = 11.84LGHH265 pKa = 5.54WWHH268 pKa = 6.35NAGNAAAAAYY278 pKa = 9.99RR279 pKa = 11.84LGGQMVYY286 pKa = 10.75GAGQAARR293 pKa = 11.84LVSATVNTARR303 pKa = 11.84TLRR306 pKa = 11.84SAAVPLLALTGG317 pKa = 3.73

Molecular weight: 33.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1029

209

503

343.0

37.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.856 ± 2.949

0.972 ± 0.155

4.373 ± 0.744

5.637 ± 0.651

2.721 ± 0.276

8.941 ± 0.634

2.527 ± 0.708

3.596 ± 0.415

3.984 ± 0.517

8.552 ± 0.13

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.228

2.721 ± 0.437

4.47 ± 0.376

3.596 ± 0.56

8.066 ± 0.93

5.539 ± 0.93

6.511 ± 1.146

7.289 ± 0.487

1.944 ± 0.342

4.082 ± 0.615
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski