Holdemania filiformis DSM 12042

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Holdemania; Holdemania filiformis

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9YCV3|B9YCV3_9FIRM ATP synthase F(0) sector subunit c OS=Holdemania filiformis DSM 12042 OX=545696 GN=HOLDEFILI_03665 PE=4 SV=1
MM1 pKa = 7.22SVVKK5 pKa = 10.67EE6 pKa = 3.69GLLYY10 pKa = 10.77SKK12 pKa = 10.11SHH14 pKa = 5.64EE15 pKa = 4.32WVEE18 pKa = 4.07TLPDD22 pKa = 3.6GTVKK26 pKa = 10.46IGISDD31 pKa = 3.9YY32 pKa = 11.44AQDD35 pKa = 3.64SLGDD39 pKa = 3.46IVFVDD44 pKa = 3.58MHH46 pKa = 6.47PAGEE50 pKa = 4.09EE51 pKa = 4.17VEE53 pKa = 4.98AEE55 pKa = 3.99GALADD60 pKa = 4.0VEE62 pKa = 4.76SVKK65 pKa = 10.72AVSEE69 pKa = 4.24IYY71 pKa = 10.7SPVAGTIKK79 pKa = 9.41EE80 pKa = 4.51TNEE83 pKa = 3.77EE84 pKa = 4.47LIDD87 pKa = 4.13APEE90 pKa = 4.34KK91 pKa = 11.06LNEE94 pKa = 4.12DD95 pKa = 4.41CYY97 pKa = 11.63GNWICVMEE105 pKa = 4.3NVGGLDD111 pKa = 3.96DD112 pKa = 4.96LLSPEE117 pKa = 4.93AYY119 pKa = 9.78SEE121 pKa = 4.18YY122 pKa = 10.23LTTLEE127 pKa = 4.27

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9YA12|B9YA12_9FIRM ABC transporter permease protein OS=Holdemania filiformis DSM 12042 OX=545696 GN=HOLDEFILI_02669 PE=3 SV=1
MM1 pKa = 6.93FRR3 pKa = 11.84ARR5 pKa = 11.84NRR7 pKa = 11.84SALVYY12 pKa = 10.4ASNRR16 pKa = 11.84RR17 pKa = 11.84TARR20 pKa = 11.84FLRR23 pKa = 11.84KK24 pKa = 9.1RR25 pKa = 11.84KK26 pKa = 9.66KK27 pKa = 10.33SRR29 pKa = 11.84APKK32 pKa = 10.16QSGLFQFII40 pKa = 3.89

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4221

0

4221

1145060

38

2945

271.3

30.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.113 ± 0.042

1.514 ± 0.016

5.355 ± 0.03

6.958 ± 0.039

4.285 ± 0.032

6.596 ± 0.035

1.932 ± 0.018

6.898 ± 0.036

5.677 ± 0.037

10.344 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.021

3.94 ± 0.029

3.792 ± 0.024

4.52 ± 0.034

4.973 ± 0.035

5.676 ± 0.028

5.287 ± 0.033

6.485 ± 0.031

1.082 ± 0.015

3.596 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski