bacterium 336/3

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; unclassified Bacteroidetes/Chlorobi group

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9DN07|A0A0M9DN07_9BACT MarR family transcriptional regulator OS=bacterium 336/3 OX=1664068 GN=AD998_00065 PE=4 SV=1
MM1 pKa = 7.42FSYY4 pKa = 10.29IGNDD8 pKa = 3.58KK9 pKa = 10.96LCLIHH14 pKa = 7.23FKK16 pKa = 10.34ILKK19 pKa = 10.1NMDD22 pKa = 4.05GIEE25 pKa = 3.93QSFEE29 pKa = 4.14EE30 pKa = 4.65VFKK33 pKa = 10.75EE34 pKa = 4.19AYY36 pKa = 8.82EE37 pKa = 3.93QEE39 pKa = 4.34EE40 pKa = 4.47FNGVVVVAHH49 pKa = 5.9QGEE52 pKa = 4.32ALYY55 pKa = 10.93EE56 pKa = 4.08EE57 pKa = 4.96AFGIADD63 pKa = 3.67IEE65 pKa = 4.48TEE67 pKa = 3.98EE68 pKa = 4.0NLAVNSIFNLASVSKK83 pKa = 10.53QFTATCIMLLYY94 pKa = 9.85EE95 pKa = 4.4QEE97 pKa = 4.15KK98 pKa = 10.75LDD100 pKa = 4.02YY101 pKa = 10.91DD102 pKa = 4.32DD103 pKa = 6.79AITTYY108 pKa = 9.4LTNLPYY114 pKa = 11.06DD115 pKa = 4.25NITIRR120 pKa = 11.84HH121 pKa = 6.21LLNHH125 pKa = 6.41TSGLPDD131 pKa = 3.36YY132 pKa = 10.49EE133 pKa = 5.56DD134 pKa = 3.01IVEE137 pKa = 4.6EE138 pKa = 4.28YY139 pKa = 10.32WDD141 pKa = 4.22GDD143 pKa = 3.77TEE145 pKa = 5.19KK146 pKa = 11.26DD147 pKa = 3.27FFTIKK152 pKa = 10.59DD153 pKa = 3.65LLEE156 pKa = 4.51IYY158 pKa = 10.42SGEE161 pKa = 4.03EE162 pKa = 3.81LEE164 pKa = 5.82LDD166 pKa = 4.18FEE168 pKa = 4.62PEE170 pKa = 3.56EE171 pKa = 4.44KK172 pKa = 10.71YY173 pKa = 10.61EE174 pKa = 3.95YY175 pKa = 11.1SNTGYY180 pKa = 10.97VFLACIVEE188 pKa = 4.45KK189 pKa = 10.79ASGMSFEE196 pKa = 5.5DD197 pKa = 4.65FIKK200 pKa = 10.54KK201 pKa = 10.03YY202 pKa = 10.25VFEE205 pKa = 4.27PLGMKK210 pKa = 10.43NSFACRR216 pKa = 11.84KK217 pKa = 8.91PNKK220 pKa = 8.4TPKK223 pKa = 10.02KK224 pKa = 8.45AAQGFTTNDD233 pKa = 3.19DD234 pKa = 3.48GDD236 pKa = 4.44YY237 pKa = 11.44EE238 pKa = 5.03DD239 pKa = 3.6YY240 pKa = 10.62TYY242 pKa = 11.35NYY244 pKa = 10.58LDD246 pKa = 4.0GLVGDD251 pKa = 4.69GNIFSSVSDD260 pKa = 3.36LVIYY264 pKa = 10.23AHH266 pKa = 7.07ALINGDD272 pKa = 4.96LLSQDD277 pKa = 3.86TLDD280 pKa = 3.94EE281 pKa = 4.61AFTPATLANGKK292 pKa = 8.35KK293 pKa = 5.41TTYY296 pKa = 10.45GFGWEE301 pKa = 4.19LEE303 pKa = 4.22GEE305 pKa = 4.51GFISHH310 pKa = 6.57EE311 pKa = 4.46GSWEE315 pKa = 4.24GYY317 pKa = 7.36NTYY320 pKa = 10.77LGIDD324 pKa = 3.78LEE326 pKa = 4.62DD327 pKa = 4.69GYY329 pKa = 11.92VFVVLDD335 pKa = 4.3NGDD338 pKa = 3.91HH339 pKa = 7.25PDD341 pKa = 3.32IADD344 pKa = 3.75LVEE347 pKa = 3.87EE348 pKa = 4.22AMEE351 pKa = 4.41GFYY354 pKa = 10.59EE355 pKa = 4.22

Molecular weight: 40.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0UYQ3|A0A0N0UYQ3_9BACT Uncharacterized protein OS=bacterium 336/3 OX=1664068 GN=AD998_16430 PE=4 SV=1
MM1 pKa = 7.04KK2 pKa = 9.96TVFLTFLVLITLPAFAQEE20 pKa = 4.73GEE22 pKa = 4.18ILHH25 pKa = 7.27DD26 pKa = 3.8GLFWGIAGTNITKK39 pKa = 10.37QNSPWGVDD47 pKa = 3.1LSVQNRR53 pKa = 11.84MYY55 pKa = 11.25KK56 pKa = 10.17NFSRR60 pKa = 11.84LQTILGTVHH69 pKa = 6.94LNYY72 pKa = 9.93RR73 pKa = 11.84RR74 pKa = 11.84PNQPWGFAVGYY85 pKa = 9.16IGGVFEE91 pKa = 4.69PLGVLHH97 pKa = 7.15IAQGRR102 pKa = 11.84INRR105 pKa = 11.84FGTLANGKK113 pKa = 9.05IPFVARR119 pKa = 11.84LSYY122 pKa = 10.92DD123 pKa = 3.7RR124 pKa = 11.84LWANPIYY131 pKa = 9.07EE132 pKa = 4.4TDD134 pKa = 2.83VRR136 pKa = 11.84TPVNHH141 pKa = 6.79RR142 pKa = 11.84WRR144 pKa = 11.84LLGQITPPITKK155 pKa = 9.92KK156 pKa = 10.53ISGFLAFEE164 pKa = 3.91PFIIRR169 pKa = 11.84RR170 pKa = 11.84GLWFSEE176 pKa = 3.91TRR178 pKa = 11.84GQAGFRR184 pKa = 11.84FLMKK188 pKa = 10.38NANLDD193 pKa = 3.27VLYY196 pKa = 10.43FNRR199 pKa = 11.84WLKK202 pKa = 10.32PINEE206 pKa = 4.25RR207 pKa = 11.84DD208 pKa = 3.3LTRR211 pKa = 11.84FEE213 pKa = 5.43HH214 pKa = 5.79VFQVIYY220 pKa = 7.44MHH222 pKa = 7.62RR223 pKa = 11.84IQFKK227 pKa = 10.29SNKK230 pKa = 5.73QTEE233 pKa = 4.14NSTNN237 pKa = 3.25

Molecular weight: 27.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4024

0

4024

1296309

38

2933

322.1

36.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.023 ± 0.037

0.828 ± 0.012

4.915 ± 0.028

6.806 ± 0.047

5.401 ± 0.035

5.906 ± 0.042

1.767 ± 0.018

8.265 ± 0.037

8.531 ± 0.053

9.549 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.019

5.978 ± 0.038

3.294 ± 0.025

4.184 ± 0.025

3.277 ± 0.023

6.224 ± 0.031

5.599 ± 0.047

5.799 ± 0.033

1.162 ± 0.016

4.293 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski