Salmonella phage SEN22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M5M1J2|A0A0M5M1J2_9CAUD Regulatory protein OS=Salmonella phage SEN22 OX=1647458 GN=SEN22_18 PE=4 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.4PNRR6 pKa = 11.84SYY8 pKa = 10.45EE9 pKa = 3.92QEE11 pKa = 3.88SIARR15 pKa = 11.84VMCAGCNKK23 pKa = 9.43QLAPDD28 pKa = 4.66EE29 pKa = 4.79IYY31 pKa = 11.13ACAEE35 pKa = 4.36CVNEE39 pKa = 3.7WLVYY43 pKa = 10.08RR44 pKa = 11.84DD45 pKa = 3.97PNGDD49 pKa = 3.31MSNEE53 pKa = 4.49DD54 pKa = 3.13IQEE57 pKa = 3.88QQ58 pKa = 3.5

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4S6T3|A0A0M4S6T3_9CAUD NinF protein OS=Salmonella phage SEN22 OX=1647458 GN=SEN22_47 PE=4 SV=1
MM1 pKa = 6.72QQFAIAGAASVRR13 pKa = 11.84PFNPILSVQHH23 pKa = 5.3SRR25 pKa = 11.84KK26 pKa = 10.2NILTGADD33 pKa = 3.88FKK35 pKa = 10.97QPRR38 pKa = 11.84MKK40 pKa = 10.71SFLEE44 pKa = 3.89KK45 pKa = 10.82LLDD48 pKa = 3.44ILKK51 pKa = 9.5QQGRR55 pKa = 11.84PP56 pKa = 3.38

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11457

46

725

208.3

23.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.331 ± 0.74

1.37 ± 0.16

6.119 ± 0.286

6.86 ± 0.421

3.247 ± 0.204

7.288 ± 0.526

1.693 ± 0.177

6.18 ± 0.271

6.101 ± 0.34

7.393 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.142

4.888 ± 0.323

3.762 ± 0.213

4.556 ± 0.364

5.961 ± 0.304

6.284 ± 0.312

5.499 ± 0.41

5.909 ± 0.301

1.562 ± 0.14

3.256 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski