Ralstonia phage RP31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ripduovirus; unclassified Ripduovirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7N1S1|A0A1L7N1S1_9CAUD Uncharacterized protein OS=Ralstonia phage RP31 OX=1923890 PE=4 SV=1
MM1 pKa = 7.37NVPIGDD7 pKa = 4.18DD8 pKa = 3.47RR9 pKa = 11.84YY10 pKa = 11.1FMQGVDD16 pKa = 3.65NLLLSEE22 pKa = 4.33IPVYY26 pKa = 10.98SDD28 pKa = 4.69DD29 pKa = 4.11EE30 pKa = 4.75YY31 pKa = 11.36EE32 pKa = 4.41LRR34 pKa = 11.84WPLIQLSYY42 pKa = 10.33PQLFSINMLGEE53 pKa = 3.89MDD55 pKa = 3.81TFLTSMPQQFEE66 pKa = 4.44VEE68 pKa = 3.87IEE70 pKa = 3.96EE71 pKa = 5.45AIDD74 pKa = 3.6LACFYY79 pKa = 11.02YY80 pKa = 10.75CDD82 pKa = 3.88WDD84 pKa = 4.03SVVAITDD91 pKa = 3.35EE92 pKa = 4.29FLNEE96 pKa = 4.3AIIEE100 pKa = 4.11EE101 pKa = 4.2ARR103 pKa = 11.84VGLIRR108 pKa = 11.84NLGCAVTAEE117 pKa = 4.31TALQDD122 pKa = 3.75SMAMLTTALQTFLNSIIGVLSRR144 pKa = 11.84TGAPAIQDD152 pKa = 3.38YY153 pKa = 10.61GCIYY157 pKa = 10.59RR158 pKa = 11.84LDD160 pKa = 4.86HH161 pKa = 7.03IDD163 pKa = 4.19DD164 pKa = 4.41FGNVYY169 pKa = 10.0FRR171 pKa = 11.84MMNPSQIFYY180 pKa = 10.06YY181 pKa = 10.63LEE183 pKa = 5.05SEE185 pKa = 4.45DD186 pKa = 3.91CQSGKK191 pKa = 9.93FRR193 pKa = 4.84

Molecular weight: 22.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L7N1X7|A0A1L7N1X7_9CAUD Putative virion structural protein OS=Ralstonia phage RP31 OX=1923890 PE=4 SV=1
MM1 pKa = 7.47LRR3 pKa = 11.84TSQRR7 pKa = 11.84KK8 pKa = 8.88EE9 pKa = 3.37ITPFDD14 pKa = 3.81PALYY18 pKa = 10.26NLQAPDD24 pKa = 2.9RR25 pKa = 11.84RR26 pKa = 11.84AFYY29 pKa = 11.0RR30 pKa = 11.84RR31 pKa = 11.84MEE33 pKa = 4.13AATILRR39 pKa = 11.84QRR41 pKa = 11.84RR42 pKa = 11.84MRR44 pKa = 11.84RR45 pKa = 11.84INKK48 pKa = 9.19YY49 pKa = 9.85LDD51 pKa = 3.36RR52 pKa = 11.84GLILLCVGVSLYY64 pKa = 10.94VAGNVLAVGYY74 pKa = 9.45EE75 pKa = 4.11RR76 pKa = 11.84LHH78 pKa = 5.94HH79 pKa = 6.34QSLLTLLGMM88 pKa = 5.16

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

287

0

287

82860

36

2502

288.7

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.247 ± 0.254

0.961 ± 0.059

6.126 ± 0.092

6.4 ± 0.169

4.182 ± 0.098

6.489 ± 0.261

2.108 ± 0.08

5.539 ± 0.092

5.486 ± 0.141

8.623 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.064

4.52 ± 0.091

4.41 ± 0.106

4.149 ± 0.127

5.124 ± 0.124

6.086 ± 0.124

5.856 ± 0.126

7.128 ± 0.132

1.22 ± 0.057

3.57 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski