Streptomyces rubidus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7682 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8RL91|A0A1H8RL91_9ACTN Glycerol uptake facilitator protein OS=Streptomyces rubidus OX=310780 GN=SAMN05216267_103548 PE=3 SV=1
MM1 pKa = 7.48SNEE4 pKa = 3.63TAEE7 pKa = 4.36ALEE10 pKa = 4.03VWIDD14 pKa = 3.41QDD16 pKa = 3.99LCTGDD21 pKa = 4.65GICVQYY27 pKa = 10.96APEE30 pKa = 4.18VFEE33 pKa = 5.9LDD35 pKa = 3.05IDD37 pKa = 3.65GLAYY41 pKa = 10.67VKK43 pKa = 10.86GPAPEE48 pKa = 4.4GEE50 pKa = 4.23EE51 pKa = 4.67AEE53 pKa = 4.6LLQTPGATVPVPLALLRR70 pKa = 11.84DD71 pKa = 3.89VADD74 pKa = 3.96SAKK77 pKa = 9.94EE78 pKa = 3.91CPGDD82 pKa = 4.18CIHH85 pKa = 6.82VRR87 pKa = 11.84RR88 pKa = 11.84TSDD91 pKa = 2.89HH92 pKa = 6.4VEE94 pKa = 4.1VYY96 pKa = 10.9GPDD99 pKa = 3.42AEE101 pKa = 4.31

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8GKS4|A0A1H8GKS4_9ACTN Tellurium resistance protein TerD OS=Streptomyces rubidus OX=310780 GN=SAMN05216267_1005144 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.88GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7682

0

7682

2598787

25

5858

338.3

35.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.453 ± 0.043

0.756 ± 0.008

5.999 ± 0.023

4.914 ± 0.033

2.636 ± 0.013

9.956 ± 0.029

2.363 ± 0.014

2.889 ± 0.019

1.756 ± 0.02

9.987 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.671 ± 0.01

1.796 ± 0.02

6.353 ± 0.028

2.887 ± 0.019

7.784 ± 0.039

5.276 ± 0.028

6.474 ± 0.038

8.396 ± 0.028

1.547 ± 0.012

2.109 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski