Candidatus Burkholderia pumila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1736 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J7JK20|A0A0J7JK20_9BURK 4Fe-4S ferredoxin iron-sulfur binding OS=Candidatus Burkholderia pumila OX=1090375 GN=BPMI_00655 PE=4 SV=1
MM1 pKa = 7.26SAVSDD6 pKa = 3.8TLTTEE11 pKa = 3.55MPMPFVFTDD20 pKa = 3.89AAADD24 pKa = 3.92RR25 pKa = 11.84VKK27 pKa = 10.87QLIDD31 pKa = 3.38EE32 pKa = 4.75EE33 pKa = 4.92GNPDD37 pKa = 3.02LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.98GFTFDD59 pKa = 4.11EE60 pKa = 5.07DD61 pKa = 4.33VNGDD65 pKa = 3.57DD66 pKa = 4.17TVMNKK71 pKa = 10.45AGVQLLVDD79 pKa = 4.04AMSYY83 pKa = 10.37QYY85 pKa = 11.49LVGAEE90 pKa = 4.01IDD92 pKa = 4.0YY93 pKa = 11.29KK94 pKa = 11.43DD95 pKa = 4.74DD96 pKa = 4.18LNGAQFVIKK105 pKa = 10.4NPNATTTCGCGSSFSVV121 pKa = 3.54

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J7JQP2|A0A0J7JQP2_9BURK INTEGRAL MEMBRANE PROTEIN (Rhomboid family) OS=Candidatus Burkholderia pumila OX=1090375 GN=BPMI_01474c PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.26QPSLTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.08RR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1736

0

1736

515917

38

1911

297.2

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.21 ± 0.08

1.035 ± 0.021

5.714 ± 0.043

5.756 ± 0.057

3.781 ± 0.044

7.563 ± 0.051

2.376 ± 0.027

5.306 ± 0.038

4.196 ± 0.048

9.715 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.026

3.222 ± 0.03

4.788 ± 0.043

3.628 ± 0.037

6.784 ± 0.051

5.765 ± 0.04

5.346 ± 0.034

7.528 ± 0.047

1.214 ± 0.024

2.464 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski