Flavobacterium arcticum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345H9I7|A0A345H9I7_9FLAO FAD-binding oxidoreductase OS=Flavobacterium arcticum OX=1784713 GN=DVK85_02990 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.32KK3 pKa = 9.85QLLFLLLLITTAASAQIVNVPDD25 pKa = 3.51VAFKK29 pKa = 10.57EE30 pKa = 4.32ALLSNYY36 pKa = 9.13TDD38 pKa = 3.98AKK40 pKa = 10.6DD41 pKa = 3.48LNGNIISLDD50 pKa = 3.57ANNDD54 pKa = 3.46GEE56 pKa = 4.49IQLSEE61 pKa = 4.06ALNVGEE67 pKa = 4.09ITIDD71 pKa = 3.3LFSFFVFSLEE81 pKa = 4.64GIGSFSNLTKK91 pKa = 10.93LSITEE96 pKa = 4.03ADD98 pKa = 3.33QLLDD102 pKa = 4.03LDD104 pKa = 4.46LTALTQLTQLSISDD118 pKa = 3.9SNEE121 pKa = 3.21FDD123 pKa = 4.13SINLNGLSNLEE134 pKa = 4.17NINFNGLTGGGDD146 pKa = 3.3SMLVLNVSGVNNLTHH161 pKa = 6.89VNFTDD166 pKa = 3.61VVFDD170 pKa = 3.8MDD172 pKa = 4.96FSQLPSLEE180 pKa = 4.14SLILNNTSVYY190 pKa = 10.66FDD192 pKa = 4.3LSANNNLSYY201 pKa = 11.42VSITNPQYY209 pKa = 11.34VDD211 pKa = 3.43YY212 pKa = 10.31FLLGNKK218 pKa = 9.22PLLTYY223 pKa = 11.12LNLNFNGGIGIVNDD237 pKa = 3.88IDD239 pKa = 5.57LSGCTALDD247 pKa = 3.63DD248 pKa = 4.12LFLRR252 pKa = 11.84FDD254 pKa = 3.65EE255 pKa = 4.84AVAIIPIPFRR265 pKa = 11.84TLNLKK270 pKa = 10.51NGISNYY276 pKa = 10.7DD277 pKa = 3.36SFTVMLNNGNLQPIYY292 pKa = 10.86VCIDD296 pKa = 3.37EE297 pKa = 5.01GNEE300 pKa = 4.06TLFSEE305 pKa = 5.22TVLNDD310 pKa = 3.59PNVLISSYY318 pKa = 11.6CNFTPGGDD326 pKa = 3.68YY327 pKa = 10.18NTITGNFTFDD337 pKa = 4.45ADD339 pKa = 5.19NDD341 pKa = 4.17GCDD344 pKa = 4.44INDD347 pKa = 3.4NLINHH352 pKa = 5.97TRR354 pKa = 11.84VDD356 pKa = 3.23ITTEE360 pKa = 3.7NDD362 pKa = 2.85EE363 pKa = 4.22EE364 pKa = 4.29FAVFSNNIGEE374 pKa = 4.38YY375 pKa = 9.62NFYY378 pKa = 10.11WGAGTFTLSPSIEE391 pKa = 4.09NNDD394 pKa = 3.27WFTITPSSATVTFEE408 pKa = 5.03DD409 pKa = 4.42DD410 pKa = 3.45NNNIEE415 pKa = 4.3TQSFCVTPNGEE426 pKa = 4.19HH427 pKa = 7.21PDD429 pKa = 3.62VEE431 pKa = 4.76VVIVPTEE438 pKa = 3.67PAQPGFNASYY448 pKa = 10.62KK449 pKa = 8.27ITYY452 pKa = 9.1RR453 pKa = 11.84NKK455 pKa = 10.29GNQTLSGNVLLTYY468 pKa = 10.76DD469 pKa = 4.22EE470 pKa = 4.65TVLDD474 pKa = 4.36YY475 pKa = 11.37VSSTSGVGAATNTFGWSYY493 pKa = 11.85SDD495 pKa = 3.55LQPFEE500 pKa = 4.17TRR502 pKa = 11.84NINLTLNVNAPTDD515 pKa = 3.87TPAVNIDD522 pKa = 3.8DD523 pKa = 5.4LLMFTANITSATGDD537 pKa = 4.01EE538 pKa = 4.47NPDD541 pKa = 3.73DD542 pKa = 3.94NTFTLAQTVVGSFDD556 pKa = 4.41PNDD559 pKa = 3.42ITCLEE564 pKa = 4.44GEE566 pKa = 4.36AVHH569 pKa = 6.16VSKK572 pKa = 10.9IGDD575 pKa = 3.58YY576 pKa = 10.9LHH578 pKa = 6.9YY579 pKa = 10.74NINFEE584 pKa = 4.42NTGTAPATFIVVKK597 pKa = 10.91NEE599 pKa = 3.49INEE602 pKa = 4.13EE603 pKa = 4.0QFDD606 pKa = 4.04VSTLQLIDD614 pKa = 3.7ASHH617 pKa = 5.82NVEE620 pKa = 4.27TRR622 pKa = 11.84ITGNKK627 pKa = 8.53VEE629 pKa = 5.19FYY631 pKa = 10.7FGNINLAANGGKK643 pKa = 10.65GNVLYY648 pKa = 10.43KK649 pKa = 10.47IKK651 pKa = 9.7TLNTLQEE658 pKa = 4.58DD659 pKa = 4.72DD660 pKa = 4.84IVMQEE665 pKa = 3.5ANIYY669 pKa = 10.21FDD671 pKa = 3.94YY672 pKa = 11.07NFPIEE677 pKa = 4.2TNEE680 pKa = 3.81ANTTFAVLSNDD691 pKa = 3.26VLTKK695 pKa = 10.58DD696 pKa = 4.86DD697 pKa = 4.28SVKK700 pKa = 10.61LYY702 pKa = 9.79PNPTTDD708 pKa = 3.26NVTIAANSAITSVQLYY724 pKa = 9.78DD725 pKa = 3.45VQGRR729 pKa = 11.84LLHH732 pKa = 6.23SRR734 pKa = 11.84VGNEE738 pKa = 3.34TSIRR742 pKa = 11.84LDD744 pKa = 3.22VSAQPSGLYY753 pKa = 9.34FVKK756 pKa = 9.5ITTDD760 pKa = 2.98NGSKK764 pKa = 10.19VEE766 pKa = 4.07KK767 pKa = 10.2LVKK770 pKa = 9.94EE771 pKa = 4.23

Molecular weight: 84.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345H9F1|A0A345H9F1_9FLAO 50S ribosomal protein L23 OS=Flavobacterium arcticum OX=1784713 GN=rplW PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNSFDD22 pKa = 3.97TITTDD27 pKa = 2.9KK28 pKa = 10.84PEE30 pKa = 4.41RR31 pKa = 11.84SLIAPKK37 pKa = 10.39KK38 pKa = 9.8NSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.86KK59 pKa = 9.44QRR61 pKa = 11.84YY62 pKa = 8.74RR63 pKa = 11.84IIDD66 pKa = 3.82FKK68 pKa = 9.82RR69 pKa = 11.84TKK71 pKa = 10.35VGIPAVVKK79 pKa = 9.88TIEE82 pKa = 3.93YY83 pKa = 10.33DD84 pKa = 3.47PNRR87 pKa = 11.84SAFISLLYY95 pKa = 10.27YY96 pKa = 10.82ADD98 pKa = 3.79GEE100 pKa = 4.22KK101 pKa = 10.52AYY103 pKa = 9.78IVAQNGLQVGQTVVSGEE120 pKa = 4.08DD121 pKa = 3.29AVPEE125 pKa = 4.25IGNAMPLSKK134 pKa = 10.36IPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCMATIGAVSNSDD199 pKa = 3.15HH200 pKa = 6.2QLVVSGKK207 pKa = 9.63AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.81PRR245 pKa = 11.84SRR247 pKa = 11.84KK248 pKa = 8.85GLPAKK253 pKa = 10.34GYY255 pKa = 6.98RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.97VNPSNKK266 pKa = 9.96YY267 pKa = 8.54IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2621

0

2621

896263

30

3500

342.0

38.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.752 ± 0.058

0.762 ± 0.015

5.738 ± 0.041

6.68 ± 0.05

4.947 ± 0.034

6.444 ± 0.044

1.676 ± 0.024

7.883 ± 0.052

7.282 ± 0.072

8.782 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.025

6.2 ± 0.046

3.457 ± 0.031

3.319 ± 0.023

3.317 ± 0.036

6.162 ± 0.042

6.419 ± 0.07

6.458 ± 0.037

1.002 ± 0.017

4.381 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski