Bacillus sp. FJAT-27916

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K9GIL8|A0A0K9GIL8_9BACI Elongation factor Tu OS=Bacillus sp. FJAT-27916 OX=1679169 GN=tuf PE=3 SV=1
MM1 pKa = 7.52QIYY4 pKa = 9.96CSKK7 pKa = 10.43CGEE10 pKa = 4.16PFSRR14 pKa = 11.84QPRR17 pKa = 11.84YY18 pKa = 10.18CSACGNKK25 pKa = 7.92TEE27 pKa = 4.56RR28 pKa = 11.84KK29 pKa = 8.02SKK31 pKa = 10.64KK32 pKa = 9.45IIIWGLALITTLCLCVMASYY52 pKa = 11.07GFYY55 pKa = 10.76LFNEE59 pKa = 4.39KK60 pKa = 10.36KK61 pKa = 10.05EE62 pKa = 4.22AEE64 pKa = 4.01QSIQDD69 pKa = 3.31KK70 pKa = 10.23PEE72 pKa = 3.76QVKK75 pKa = 10.12EE76 pKa = 4.23VVKK79 pKa = 9.53KK80 pKa = 8.93TKK82 pKa = 10.52KK83 pKa = 9.28EE84 pKa = 3.97APKK87 pKa = 10.0PALQKK92 pKa = 10.02TSTAPKK98 pKa = 9.86DD99 pKa = 3.41VSQIIEE105 pKa = 3.96EE106 pKa = 4.28SLSKK110 pKa = 11.03VFTISNGTSQGSGFLINKK128 pKa = 9.71NGDD131 pKa = 3.31ILTNAHH137 pKa = 4.85VVEE140 pKa = 4.83GNTLAIVKK148 pKa = 9.25DD149 pKa = 3.93HH150 pKa = 6.94NGTEE154 pKa = 4.19HH155 pKa = 6.24QGTVIGYY162 pKa = 9.32SNEE165 pKa = 3.33VDD167 pKa = 2.81IALIRR172 pKa = 11.84VPNLANQTPLSIEE185 pKa = 4.27TTDD188 pKa = 3.29KK189 pKa = 11.27SQLGDD194 pKa = 3.74EE195 pKa = 4.94IIALGSPRR203 pKa = 11.84GLEE206 pKa = 3.92NSATLGNISGVDD218 pKa = 3.12RR219 pKa = 11.84TFVIEE224 pKa = 3.6PHH226 pKa = 6.72LYY228 pKa = 10.55DD229 pKa = 5.14GIYY232 pKa = 10.08QISAPLAPGSSGGPLLDD249 pKa = 3.58RR250 pKa = 11.84KK251 pKa = 7.48TEE253 pKa = 3.91KK254 pKa = 10.9VIAINSAKK262 pKa = 10.2MNGEE266 pKa = 4.06DD267 pKa = 4.33NIGFSIPLYY276 pKa = 10.65KK277 pKa = 10.23IMNTVTQWSSAPMSEE292 pKa = 4.6QEE294 pKa = 4.35INQLFYY300 pKa = 11.53NEE302 pKa = 4.83AGVFYY307 pKa = 11.37YY308 pKa = 10.66EE309 pKa = 4.65DD310 pKa = 4.09LYY312 pKa = 11.74GNEE315 pKa = 4.96GYY317 pKa = 10.61FDD319 pKa = 3.79GGSYY323 pKa = 10.19EE324 pKa = 4.24EE325 pKa = 5.43EE326 pKa = 3.85YY327 pKa = 11.09DD328 pKa = 4.41SYY330 pKa = 11.85YY331 pKa = 10.96EE332 pKa = 4.39DD333 pKa = 3.99YY334 pKa = 10.8EE335 pKa = 4.17YY336 pKa = 10.62DD337 pKa = 3.64YY338 pKa = 11.59DD339 pKa = 4.68DD340 pKa = 5.2SEE342 pKa = 4.73FEE344 pKa = 4.07EE345 pKa = 5.84DD346 pKa = 3.39YY347 pKa = 11.44SEE349 pKa = 4.61YY350 pKa = 10.95DD351 pKa = 3.52DD352 pKa = 5.31YY353 pKa = 11.71EE354 pKa = 4.6YY355 pKa = 11.53DD356 pKa = 3.4HH357 pKa = 7.57DD358 pKa = 4.22EE359 pKa = 5.48GYY361 pKa = 11.29DD362 pKa = 3.88DD363 pKa = 5.38MYY365 pKa = 11.58DD366 pKa = 3.6DD367 pKa = 3.65SDD369 pKa = 4.46YY370 pKa = 11.67YY371 pKa = 11.09EE372 pKa = 4.91EE373 pKa = 5.85DD374 pKa = 3.68PEE376 pKa = 4.82EE377 pKa = 5.24DD378 pKa = 3.52DD379 pKa = 4.41SLMIEE384 pKa = 4.3EE385 pKa = 5.14DD386 pKa = 3.57SEE388 pKa = 4.11EE389 pKa = 5.14AYY391 pKa = 10.81DD392 pKa = 3.73DD393 pKa = 4.03TLVEE397 pKa = 4.07EE398 pKa = 4.76SDD400 pKa = 4.44LEE402 pKa = 4.32EE403 pKa = 5.02EE404 pKa = 4.95NDD406 pKa = 4.28DD407 pKa = 5.67DD408 pKa = 6.17DD409 pKa = 6.59IEE411 pKa = 4.21MDD413 pKa = 3.58PYY415 pKa = 11.33GNKK418 pKa = 9.88EE419 pKa = 4.12DD420 pKa = 3.9VDD422 pKa = 4.63SDD424 pKa = 4.15VSDD427 pKa = 5.49DD428 pKa = 4.57YY429 pKa = 11.95LDD431 pKa = 3.36QQ432 pKa = 5.83

Molecular weight: 48.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K9GBR2|A0A0K9GBR2_9BACI Nucleoside diphosphate kinase OS=Bacillus sp. FJAT-27916 OX=1679169 GN=ndk PE=3 SV=1
MM1 pKa = 7.26QGGEE5 pKa = 4.35EE6 pKa = 4.34PCGFPAIDD14 pKa = 3.83YY15 pKa = 8.33VWSGRR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.03GKK24 pKa = 10.68GMVEE28 pKa = 3.77RR29 pKa = 11.84WEE31 pKa = 4.48MPCSSRR37 pKa = 11.84LFPGQGIYY45 pKa = 10.19RR46 pKa = 11.84AVEE49 pKa = 3.62GWEE52 pKa = 3.77RR53 pKa = 11.84GGRR56 pKa = 11.84SHH58 pKa = 7.27AVPTQCPYY66 pKa = 10.65KK67 pKa = 10.81GYY69 pKa = 10.24IGWGKK74 pKa = 10.2GGHH77 pKa = 5.31RR78 pKa = 11.84QRR80 pKa = 11.84KK81 pKa = 5.67PTQCPGKK88 pKa = 10.74GYY90 pKa = 10.23IGWGKK95 pKa = 10.26GGHH98 pKa = 5.24RR99 pKa = 11.84QRR101 pKa = 11.84NPIQCPGKK109 pKa = 10.82GYY111 pKa = 10.29IGWGKK116 pKa = 10.2GGHH119 pKa = 5.31RR120 pKa = 11.84QRR122 pKa = 11.84KK123 pKa = 5.67PTQCPGKK130 pKa = 10.74GYY132 pKa = 10.14IGWGKK137 pKa = 10.58GEE139 pKa = 3.85HH140 pKa = 5.8RR141 pKa = 11.84QRR143 pKa = 11.84KK144 pKa = 6.12PTQCPGKK151 pKa = 10.74GYY153 pKa = 10.23IGWGKK158 pKa = 10.2GGHH161 pKa = 5.31RR162 pKa = 11.84QRR164 pKa = 11.84KK165 pKa = 5.95PTQCPYY171 pKa = 10.48KK172 pKa = 10.77GYY174 pKa = 10.24IGWGKK179 pKa = 10.26GGHH182 pKa = 5.24RR183 pKa = 11.84QRR185 pKa = 11.84NPIQCPGKK193 pKa = 10.73GYY195 pKa = 10.27IGG197 pKa = 3.56

Molecular weight: 21.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3770

0

3770

1089385

26

1873

289.0

32.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.484 ± 0.041

0.75 ± 0.012

5.144 ± 0.037

7.737 ± 0.048

4.377 ± 0.039

7.207 ± 0.039

2.072 ± 0.021

7.79 ± 0.038

6.738 ± 0.04

9.716 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.991 ± 0.019

4.023 ± 0.022

3.604 ± 0.022

3.495 ± 0.026

4.254 ± 0.035

5.917 ± 0.032

5.282 ± 0.03

6.733 ± 0.032

1.033 ± 0.016

3.653 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski