Mycobacterium grossiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5542 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E8Q0S5|A0A1E8Q0S5_9MYCO Protein rarD OS=Mycobacterium grossiae OX=1552759 GN=BEL07_22625 PE=3 SV=1
MM1 pKa = 7.2NVDD4 pKa = 3.36SSGGAITSEE13 pKa = 4.16HH14 pKa = 6.08RR15 pKa = 11.84TSDD18 pKa = 3.51DD19 pKa = 2.98TSAPTTVSQPAPTTDD34 pKa = 3.39SQSVSPAVSPAVSQPQDD51 pKa = 3.26APAAPASAPAKK62 pKa = 10.49NDD64 pKa = 3.37DD65 pKa = 4.18PAPAPTARR73 pKa = 11.84PAAPGAVVHH82 pKa = 7.01ADD84 pKa = 3.6TVSVGSTDD92 pKa = 3.36ATAVAPPPAAAVVSVPAASLDD113 pKa = 3.55ATAGSVASEE122 pKa = 4.39PEE124 pKa = 4.12SAALQQEE131 pKa = 4.59STVVSVLAAAVAPFVLPGPATPAASPLLWAVLAWTRR167 pKa = 11.84RR168 pKa = 11.84EE169 pKa = 4.1AEE171 pKa = 3.79VDD173 pKa = 3.57GSSEE177 pKa = 4.23TLRR180 pKa = 11.84TTAAAAPADD189 pKa = 3.92FEE191 pKa = 4.66VEE193 pKa = 3.52EE194 pKa = 4.4DD195 pKa = 3.51QPLFGNVLDD204 pKa = 4.43LHH206 pKa = 6.86PDD208 pKa = 3.44VTPGTVTVGDD218 pKa = 4.06GTAPAHH224 pKa = 5.84GTLTLDD230 pKa = 3.31GSGNFVYY237 pKa = 10.38TPDD240 pKa = 3.6QDD242 pKa = 4.69FSGDD246 pKa = 3.62DD247 pKa = 3.23QFGYY251 pKa = 9.24TLTDD255 pKa = 3.53FDD257 pKa = 4.62GVVVTATARR266 pKa = 11.84ITVTPAPDD274 pKa = 3.3VPVGSDD280 pKa = 2.72ADD282 pKa = 3.63VRR284 pKa = 11.84TDD286 pKa = 3.19EE287 pKa = 4.71DD288 pKa = 4.27TPITSTLPAAGDD300 pKa = 3.45VDD302 pKa = 4.25GDD304 pKa = 3.87EE305 pKa = 4.75LVYY308 pKa = 10.81AVAQSPQSGTVVVAADD324 pKa = 3.54GAYY327 pKa = 9.4TYY329 pKa = 10.55TPAPNFYY336 pKa = 10.62GVDD339 pKa = 3.27SFGYY343 pKa = 7.91TVSDD347 pKa = 3.46GTFTVGYY354 pKa = 9.26VVTVTVAPVDD364 pKa = 4.07DD365 pKa = 5.16APVALDD371 pKa = 4.24DD372 pKa = 4.39SVSGPEE378 pKa = 3.84DD379 pKa = 3.5SEE381 pKa = 4.42FTGVVPVTEE390 pKa = 3.92VDD392 pKa = 3.65GDD394 pKa = 3.93VLTYY398 pKa = 10.72GIDD401 pKa = 3.09RR402 pKa = 11.84GPQHH406 pKa = 6.07GTVTLGADD414 pKa = 3.18GRR416 pKa = 11.84YY417 pKa = 9.82VYY419 pKa = 10.47TPAADD424 pKa = 3.47FHH426 pKa = 6.58GADD429 pKa = 3.25SFTYY433 pKa = 9.52TVTDD437 pKa = 3.62GTTAPSTGTIAFTVTPVNDD456 pKa = 4.07PPRR459 pKa = 11.84SEE461 pKa = 4.53HH462 pKa = 6.8LDD464 pKa = 3.26VSTAEE469 pKa = 3.99DD470 pKa = 3.57TPYY473 pKa = 11.3DD474 pKa = 3.76GVLPTSDD481 pKa = 4.37PDD483 pKa = 4.27GDD485 pKa = 4.14PLTYY489 pKa = 10.73DD490 pKa = 3.82LFGTPVHH497 pKa = 5.74GTVIIRR503 pKa = 11.84PDD505 pKa = 3.06GSFTYY510 pKa = 10.47RR511 pKa = 11.84PDD513 pKa = 3.33ANYY516 pKa = 11.0NGADD520 pKa = 3.3EE521 pKa = 4.45FTYY524 pKa = 10.76HH525 pKa = 7.41VMDD528 pKa = 3.99GTTYY532 pKa = 8.39GQYY535 pKa = 10.22VVRR538 pKa = 11.84ITVTAVNDD546 pKa = 3.78VPVTTPAAFTTGPGVAVTDD565 pKa = 3.95TVAWHH570 pKa = 6.63ASDD573 pKa = 4.78VDD575 pKa = 3.98VADD578 pKa = 4.25EE579 pKa = 4.33LTFTAAAPTAGTVTMGADD597 pKa = 3.52GQFTYY602 pKa = 10.19TPRR605 pKa = 11.84PGFSGTDD612 pKa = 3.04TFVFTVTDD620 pKa = 3.64TAGATATGVVSVTVANQQPVAYY642 pKa = 9.58DD643 pKa = 3.19DD644 pKa = 5.28GYY646 pKa = 11.47QVTPGATLTVDD657 pKa = 3.41AAHH660 pKa = 6.97GLLANDD666 pKa = 4.32VDD668 pKa = 4.53PGDD671 pKa = 4.37PLTVTSVTQPTHH683 pKa = 4.79GTVTVAGDD691 pKa = 3.47GSFTYY696 pKa = 10.21TPTAGFSGDD705 pKa = 3.2DD706 pKa = 3.29WFSYY710 pKa = 10.33TITDD714 pKa = 3.68SAGATATATVFFGVAPASNLPPIAPDD740 pKa = 2.96WTLTTEE746 pKa = 4.17ADD748 pKa = 3.14VDD750 pKa = 4.13VTFDD754 pKa = 3.78PLAGVTDD761 pKa = 4.33PNGDD765 pKa = 3.92VLWVRR770 pKa = 11.84DD771 pKa = 4.11GEE773 pKa = 4.43QPAHH777 pKa = 6.65GFVQQYY783 pKa = 9.57TNGTIAYY790 pKa = 8.86VPDD793 pKa = 3.62GTFVGTDD800 pKa = 2.89GFTYY804 pKa = 10.05EE805 pKa = 4.21VHH807 pKa = 7.5DD808 pKa = 3.96GTNVVTVRR816 pKa = 11.84VTVITSPSTAPVATGDD832 pKa = 3.83EE833 pKa = 4.31VSMPEE838 pKa = 4.21GGTVTIDD845 pKa = 3.24PLANDD850 pKa = 4.21TFIGTPTVEE859 pKa = 5.05LISTAQYY866 pKa = 8.99GTVTVDD872 pKa = 2.77AATGKK877 pKa = 8.53LTYY880 pKa = 10.44VATAGTNALGDD891 pKa = 3.71SFAYY895 pKa = 10.29QITDD899 pKa = 2.9EE900 pKa = 4.2RR901 pKa = 11.84GRR903 pKa = 11.84WSVASVDD910 pKa = 3.34ITFTAGNRR918 pKa = 11.84PPVATDD924 pKa = 3.85DD925 pKa = 3.96VAATPIGTPVSIDD938 pKa = 3.44VLANDD943 pKa = 4.08YY944 pKa = 11.44DD945 pKa = 4.85RR946 pKa = 11.84DD947 pKa = 3.97GTTLTVSVVSQPEE960 pKa = 3.88FGGTAIARR968 pKa = 11.84KK969 pKa = 10.07DD970 pKa = 3.36GTIDD974 pKa = 3.49FTPTKK979 pKa = 9.41GYY981 pKa = 11.04VGMASFSYY989 pKa = 10.42EE990 pKa = 3.95VTDD993 pKa = 3.51EE994 pKa = 4.24SGAVARR1000 pKa = 11.84ATVTVTVYY1008 pKa = 11.11DD1009 pKa = 4.18PTPPDD1014 pKa = 3.93APVKK1018 pKa = 10.51SYY1020 pKa = 9.76TVGKK1024 pKa = 10.42GRR1026 pKa = 11.84TLTVSLGDD1034 pKa = 3.65GVLAGSGYY1042 pKa = 10.18QGWLTGLEE1050 pKa = 4.08TGPAHH1055 pKa = 6.33GVLRR1059 pKa = 11.84LAEE1062 pKa = 5.09DD1063 pKa = 4.02GSFTYY1068 pKa = 9.86TPRR1071 pKa = 11.84EE1072 pKa = 4.09GFVGTDD1078 pKa = 2.89SFVFYY1083 pKa = 10.99VHH1085 pKa = 6.24TRR1087 pKa = 11.84EE1088 pKa = 4.23QGDD1091 pKa = 4.26GPFTATITVTEE1102 pKa = 5.25DD1103 pKa = 4.04GPGTPPNDD1111 pKa = 3.4AVAVALSSSDD1121 pKa = 3.28TAYY1124 pKa = 8.34GTCGGDD1130 pKa = 2.8WFAGRR1135 pKa = 11.84DD1136 pKa = 3.95DD1137 pKa = 5.0PASTWPTCLFLGRR1150 pKa = 11.84PEE1152 pKa = 4.31DD1153 pKa = 3.65VV1154 pKa = 3.47

Molecular weight: 117.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E8Q1F6|A0A1E8Q1F6_9MYCO Molybdopterin molybdenumtransferase OS=Mycobacterium grossiae OX=1552759 GN=BEL07_20890 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSSRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.72GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5542

0

5542

1755592

29

4278

316.8

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.753 ± 0.047

0.754 ± 0.009

6.683 ± 0.029

5.017 ± 0.029

2.966 ± 0.016

8.991 ± 0.034

2.207 ± 0.017

3.825 ± 0.019

1.895 ± 0.023

9.846 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.916 ± 0.015

1.973 ± 0.018

5.806 ± 0.029

2.712 ± 0.017

7.52 ± 0.041

5.092 ± 0.024

6.311 ± 0.03

9.168 ± 0.036

1.493 ± 0.014

2.074 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski