Podoviridae sp. ctdc61

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385E2G4|A0A385E2G4_9CAUD YvrJ family protein OS=Podoviridae sp. ctdc61 OX=2675449 PE=4 SV=1
MM1 pKa = 7.44TYY3 pKa = 10.27DD4 pKa = 3.78YY5 pKa = 11.14KK6 pKa = 11.42NKK8 pKa = 10.15AVNVRR13 pKa = 11.84QLNNYY18 pKa = 8.25MYY20 pKa = 11.1AKK22 pKa = 7.97TLSMFEE28 pKa = 4.17YY29 pKa = 10.29QGLPPSIPVRR39 pKa = 11.84EE40 pKa = 4.35LEE42 pKa = 4.11RR43 pKa = 11.84LLQIGGYY50 pKa = 10.61AFITEE55 pKa = 4.24APNGKK60 pKa = 9.9LYY62 pKa = 10.84AFHH65 pKa = 7.17GGLGGVPCPYY75 pKa = 10.66GNPTEE80 pKa = 4.43IVIANPALNFYY91 pKa = 8.42KK92 pKa = 10.11TLKK95 pKa = 10.57LKK97 pKa = 10.02TDD99 pKa = 3.49GVLIRR104 pKa = 11.84SDD106 pKa = 4.54DD107 pKa = 3.62MALGLDD113 pKa = 3.49PLFYY117 pKa = 10.37KK118 pKa = 10.81CNMLLAEE125 pKa = 4.67NDD127 pKa = 3.14INMILHH133 pKa = 6.91GYY135 pKa = 6.18STRR138 pKa = 11.84MQKK141 pKa = 10.58LISASDD147 pKa = 3.4DD148 pKa = 3.44KK149 pKa = 11.28TRR151 pKa = 11.84EE152 pKa = 4.05SADD155 pKa = 3.47SYY157 pKa = 10.57LKK159 pKa = 10.62KK160 pKa = 10.99VIDD163 pKa = 4.07GEE165 pKa = 4.36VSIVAEE171 pKa = 3.89NALFDD176 pKa = 3.71GVKK179 pKa = 9.62IHH181 pKa = 5.96GTSGDD186 pKa = 3.96SVGVTGMVEE195 pKa = 3.19YY196 pKa = 10.08HH197 pKa = 6.18QYY199 pKa = 10.74VKK201 pKa = 10.9SLMLSEE207 pKa = 5.02IGLSTSFNMKK217 pKa = 9.31KK218 pKa = 10.34EE219 pKa = 4.01RR220 pKa = 11.84MITAEE225 pKa = 4.46VDD227 pKa = 3.42QQDD230 pKa = 4.07DD231 pKa = 4.02STFPFVYY238 pKa = 11.02NMMKK242 pKa = 10.25CRR244 pKa = 11.84LHH246 pKa = 7.38GMEE249 pKa = 5.93RR250 pKa = 11.84INEE253 pKa = 4.13MYY255 pKa = 8.52GTSIEE260 pKa = 4.07VDD262 pKa = 4.66FGSVWNLKK270 pKa = 9.73NKK272 pKa = 9.96QLVDD276 pKa = 4.52DD277 pKa = 5.31IIGNNEE283 pKa = 4.42DD284 pKa = 3.9EE285 pKa = 4.99LSTIDD290 pKa = 4.82GEE292 pKa = 4.36IDD294 pKa = 3.47NEE296 pKa = 4.72LVQLDD301 pKa = 4.27NDD303 pKa = 4.1DD304 pKa = 3.95TTDD307 pKa = 3.81DD308 pKa = 4.12DD309 pKa = 5.9LIDD312 pKa = 4.53RR313 pKa = 11.84EE314 pKa = 4.19LAEE317 pKa = 5.44LEE319 pKa = 4.43TLSSALDD326 pKa = 3.53KK327 pKa = 10.84PLEE330 pKa = 4.58DD331 pKa = 4.69EE332 pKa = 5.59DD333 pKa = 5.07VEE335 pKa = 4.24LDD337 pKa = 3.53KK338 pKa = 11.68LIDD341 pKa = 3.75EE342 pKa = 4.84VVDD345 pKa = 3.59EE346 pKa = 4.75TVDD349 pKa = 3.36EE350 pKa = 4.28EE351 pKa = 4.69LFEE354 pKa = 5.3KK355 pKa = 10.73EE356 pKa = 4.02IDD358 pKa = 4.0DD359 pKa = 4.93GLAEE363 pKa = 5.03LDD365 pKa = 3.55PTKK368 pKa = 10.8TEE370 pKa = 4.7DD371 pKa = 4.21EE372 pKa = 4.39DD373 pKa = 5.62DD374 pKa = 3.92EE375 pKa = 4.98PEE377 pKa = 4.8PEE379 pKa = 4.13TSVTDD384 pKa = 3.49IEE386 pKa = 4.35DD387 pKa = 3.8TEE389 pKa = 4.64RR390 pKa = 11.84DD391 pKa = 3.5IPEE394 pKa = 4.27TEE396 pKa = 4.36LDD398 pKa = 4.07KK399 pKa = 11.65DD400 pKa = 5.31DD401 pKa = 6.35DD402 pKa = 4.47DD403 pKa = 4.11EE404 pKa = 4.68QNAVVEE410 pKa = 5.12DD411 pKa = 3.79IVDD414 pKa = 4.2DD415 pKa = 4.17SGTDD419 pKa = 3.38NEE421 pKa = 4.33QSQEE425 pKa = 3.99EE426 pKa = 4.72VVSVEE431 pKa = 4.64VIDD434 pKa = 3.95TVIDD438 pKa = 3.5VLEE441 pKa = 4.49SIKK444 pKa = 10.22TSTNDD449 pKa = 3.3EE450 pKa = 4.08IEE452 pKa = 4.79EE453 pKa = 4.38ISDD456 pKa = 3.64TLNTSKK462 pKa = 11.19DD463 pKa = 3.2KK464 pKa = 11.6DD465 pKa = 3.73NASTT469 pKa = 3.54

Molecular weight: 52.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385E503|A0A385E503_9CAUD Uncharacterized protein OS=Podoviridae sp. ctdc61 OX=2675449 PE=4 SV=1
MM1 pKa = 7.87ALPAILIGVSRR12 pKa = 11.84LALGTQRR19 pKa = 11.84FRR21 pKa = 11.84SAATVTTGIGVRR33 pKa = 11.84SNVVRR38 pKa = 11.84VPTIAAQQQQNKK50 pKa = 9.03KK51 pKa = 9.38RR52 pKa = 11.84LRR54 pKa = 11.84EE55 pKa = 4.02LASSLASKK63 pKa = 10.3ANKK66 pKa = 9.16RR67 pKa = 11.84VRR69 pKa = 11.84RR70 pKa = 11.84LEE72 pKa = 4.02KK73 pKa = 10.94NGFTDD78 pKa = 3.42QSAYY82 pKa = 8.46RR83 pKa = 11.84TYY85 pKa = 10.97VEE87 pKa = 4.24SGGKK91 pKa = 10.09FSVKK95 pKa = 9.8GKK97 pKa = 9.42NEE99 pKa = 3.86KK100 pKa = 10.32EE101 pKa = 3.63ILAEE105 pKa = 3.89LEE107 pKa = 4.07RR108 pKa = 11.84VKK110 pKa = 10.91KK111 pKa = 10.55FLGDD115 pKa = 3.45EE116 pKa = 4.21SSTVSGINKK125 pKa = 8.69QAEE128 pKa = 4.42SVAKK132 pKa = 10.24RR133 pKa = 11.84LNIKK137 pKa = 10.35YY138 pKa = 8.36KK139 pKa = 8.14TFKK142 pKa = 10.31EE143 pKa = 4.0LRR145 pKa = 11.84EE146 pKa = 3.91KK147 pKa = 10.66NQAIFKK153 pKa = 8.93LLPKK157 pKa = 8.74ITEE160 pKa = 4.01YY161 pKa = 10.89MKK163 pKa = 10.38MSSNTYY169 pKa = 8.74TEE171 pKa = 4.06YY172 pKa = 11.28DD173 pKa = 3.31SDD175 pKa = 3.95QVIDD179 pKa = 4.02EE180 pKa = 4.32VTDD183 pKa = 3.72YY184 pKa = 11.22VDD186 pKa = 4.25EE187 pKa = 4.73VMAQLKK193 pKa = 10.63DD194 pKa = 3.5GEE196 pKa = 4.54TLDD199 pKa = 4.31IEE201 pKa = 4.82QAIQDD206 pKa = 3.46IEE208 pKa = 3.99QRR210 pKa = 11.84IIKK213 pKa = 9.68LDD215 pKa = 3.44KK216 pKa = 10.66KK217 pKa = 10.38SISVKK222 pKa = 10.17RR223 pKa = 11.84SNIPSFYY230 pKa = 10.74KK231 pKa = 10.51PP232 pKa = 3.21

Molecular weight: 26.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

3940

90

1186

394.0

44.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.924 ± 0.265

0.635 ± 0.297

7.766 ± 0.728

6.244 ± 0.74

4.01 ± 0.333

5.914 ± 0.535

1.168 ± 0.221

7.766 ± 0.533

6.777 ± 0.487

7.919 ± 0.439

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.35

6.777 ± 0.419

3.756 ± 0.762

2.157 ± 0.339

3.325 ± 0.365

6.777 ± 0.328

7.893 ± 0.407

8.02 ± 0.69

0.939 ± 0.134

5.0 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski