Cryptobacterium curtum (strain ATCC 700683 / DSM 15641 / CCUG 43107 / 12-3)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Cryptobacterium; Cryptobacterium curtum

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7MMP9|C7MMP9_CRYCD Uncharacterized protein OS=Cryptobacterium curtum (strain ATCC 700683 / DSM 15641 / CCUG 43107 / 12-3) OX=469378 GN=Ccur_04670 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 9.95DD3 pKa = 3.45ARR5 pKa = 11.84WRR7 pKa = 11.84WIATAACCVLIVVSAWTLGSYY28 pKa = 10.71FLGSSGASEE37 pKa = 5.09DD38 pKa = 4.2LFTTVAPAEE47 pKa = 4.28SDD49 pKa = 3.04ASSGVTTTGDD59 pKa = 4.7LIPKK63 pKa = 9.86QEE65 pKa = 5.52DD66 pKa = 3.38DD67 pKa = 4.29TADD70 pKa = 3.49QSDD73 pKa = 3.93GAAGEE78 pKa = 4.55DD79 pKa = 3.9TGSSDD84 pKa = 5.59DD85 pKa = 3.91PTPPLAAADD94 pKa = 3.72SAYY97 pKa = 9.51ATQSGDD103 pKa = 3.6PQSSDD108 pKa = 2.95SSSRR112 pKa = 11.84SSDD115 pKa = 3.05GSSVAGGSSASSATTSGSSSSSSSSGDD142 pKa = 3.28QLTHH146 pKa = 5.36IQVNITVDD154 pKa = 3.52GTAAGSNISSALLSIPAGSTVYY176 pKa = 10.58DD177 pKa = 3.63ALKK180 pKa = 9.81ATGASVNARR189 pKa = 11.84STVYY193 pKa = 10.87GMYY196 pKa = 10.24VAGINGLAEE205 pKa = 4.29KK206 pKa = 10.26EE207 pKa = 4.08YY208 pKa = 10.5GAQSGWIYY216 pKa = 10.57YY217 pKa = 10.55VNGQFADD224 pKa = 4.29RR225 pKa = 11.84ACDD228 pKa = 2.99RR229 pKa = 11.84WRR231 pKa = 11.84LSEE234 pKa = 3.86GDD236 pKa = 3.74YY237 pKa = 11.14VKK239 pKa = 10.11WVYY242 pKa = 11.04VSDD245 pKa = 3.7NN246 pKa = 3.35

Molecular weight: 25.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7MMP7|C7MMP7_CRYCD ABC-type cobalt transport system ATPase component OS=Cryptobacterium curtum (strain ATCC 700683 / DSM 15641 / CCUG 43107 / 12-3) OX=469378 GN=Ccur_04650 PE=4 SV=1
MM1 pKa = 7.6ARR3 pKa = 11.84RR4 pKa = 11.84TSEE7 pKa = 3.76HH8 pKa = 6.15EE9 pKa = 3.64ARR11 pKa = 11.84ILDD14 pKa = 3.73FEE16 pKa = 4.41EE17 pKa = 3.89ARR19 pKa = 11.84RR20 pKa = 11.84PARR23 pKa = 11.84EE24 pKa = 3.49QSTRR28 pKa = 11.84AARR31 pKa = 11.84PSTRR35 pKa = 11.84RR36 pKa = 11.84ATRR39 pKa = 11.84TASSRR44 pKa = 11.84SSARR48 pKa = 11.84GEE50 pKa = 4.33SSSQPARR57 pKa = 11.84RR58 pKa = 11.84STGAASSSQAGSVRR72 pKa = 11.84RR73 pKa = 11.84SGRR76 pKa = 11.84RR77 pKa = 11.84IQSSARR83 pKa = 11.84EE84 pKa = 3.84EE85 pKa = 4.45SFCRR89 pKa = 11.84SASTASSRR97 pKa = 11.84HH98 pKa = 3.9STRR101 pKa = 11.84PHH103 pKa = 5.21VSARR107 pKa = 11.84QQQSGRR113 pKa = 11.84ITSFFKK119 pKa = 10.83GLQKK123 pKa = 10.52KK124 pKa = 10.1RR125 pKa = 11.84LDD127 pKa = 3.38KK128 pKa = 11.23RR129 pKa = 11.84FDD131 pKa = 3.42RR132 pKa = 11.84SVRR135 pKa = 11.84TGDD138 pKa = 3.59DD139 pKa = 3.45TTHH142 pKa = 6.65EE143 pKa = 4.62SSNSARR149 pKa = 11.84PKK151 pKa = 8.79AALYY155 pKa = 6.58STHH158 pKa = 6.5MGRR161 pKa = 11.84VHH163 pKa = 6.9RR164 pKa = 11.84KK165 pKa = 7.55SSRR168 pKa = 11.84MQNTAARR175 pKa = 11.84TTSRR179 pKa = 11.84AGSASIATSSRR190 pKa = 11.84QRR192 pKa = 11.84WVVPVCVVAFLVLCGAFLYY211 pKa = 10.87APAKK215 pKa = 10.2DD216 pKa = 4.0YY217 pKa = 11.54YY218 pKa = 10.18IASRR222 pKa = 11.84DD223 pKa = 3.54AAKK226 pKa = 8.92TQAEE230 pKa = 4.5YY231 pKa = 11.39NRR233 pKa = 11.84VSQEE237 pKa = 3.62NSQLQSDD244 pKa = 4.61VEE246 pKa = 4.38SLSTDD251 pKa = 2.91EE252 pKa = 5.39GIEE255 pKa = 3.78DD256 pKa = 4.36RR257 pKa = 11.84ARR259 pKa = 11.84EE260 pKa = 3.85DD261 pKa = 3.56YY262 pKa = 11.07GWVKK266 pKa = 10.59EE267 pKa = 4.29GEE269 pKa = 4.23NSVSVSGLSSDD280 pKa = 4.65RR281 pKa = 11.84DD282 pKa = 3.46QSTNASSKK290 pKa = 10.89ADD292 pKa = 3.25AASEE296 pKa = 4.39VKK298 pKa = 10.69APTTWYY304 pKa = 10.75SGILDD309 pKa = 3.77AFFGYY314 pKa = 10.26KK315 pKa = 10.28DD316 pKa = 3.36SS317 pKa = 3.98

Molecular weight: 34.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1355

0

1355

470126

26

2400

347.0

37.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.275 ± 0.078

1.503 ± 0.032

6.109 ± 0.061

6.019 ± 0.063

3.702 ± 0.045

7.632 ± 0.056

2.106 ± 0.028

5.81 ± 0.053

3.746 ± 0.052

8.976 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.033

3.122 ± 0.044

4.106 ± 0.039

3.538 ± 0.042

5.665 ± 0.061

6.689 ± 0.069

5.92 ± 0.07

7.754 ± 0.054

1.042 ± 0.024

2.757 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski