Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius; Roseovarius nubinhibens

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A3SRC4|A3SRC4_ROSNI C4-dicarboxylate transport transcriptional regulatory protein DctD OS=Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM) OX=89187 GN=ISM_11185 PE=4 SV=1
MM1 pKa = 7.8PKK3 pKa = 10.3FIFAYY8 pKa = 10.24HH9 pKa = 6.75GGSAPNPDD17 pKa = 4.32DD18 pKa = 4.0DD19 pKa = 6.07AEE21 pKa = 4.11IARR24 pKa = 11.84IMGAWQGWLEE34 pKa = 4.1GMGAACLDD42 pKa = 3.4MGNPVGLSKK51 pKa = 9.82TVTADD56 pKa = 3.19GLRR59 pKa = 11.84DD60 pKa = 3.85DD61 pKa = 5.59GGANPLSGYY70 pKa = 8.86TIVEE74 pKa = 4.2AADD77 pKa = 3.27MAAACDD83 pKa = 3.87LAQGCPILEE92 pKa = 4.42GGAGSVEE99 pKa = 3.97VAPIVEE105 pKa = 4.32MM106 pKa = 4.93

Molecular weight:
10.74 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A3SIU1|A3SIU1_ROSNI Metallophos domain-containing protein OS=Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM) OX=89187 GN=ISM_03245 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight:
5.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3545

0

3545

1098103

18

2140

309.8

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.408 ± 0.056

0.907 ± 0.013

5.963 ± 0.04

6.205 ± 0.04

3.628 ± 0.026

8.906 ± 0.043

2.16 ± 0.023

5.065 ± 0.032

3.066 ± 0.034

10.21 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.02

2.429 ± 0.021

5.111 ± 0.033

3.202 ± 0.021

6.915 ± 0.042

5.059 ± 0.029

5.234 ± 0.03

7.002 ± 0.034

1.405 ± 0.018

2.281 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski