Eubacterium sp. CAG:38

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7HBY2|R7HBY2_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:38 OX=1262889 GN=BN634_00225 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84LIYY5 pKa = 10.25SDD7 pKa = 5.13YY8 pKa = 11.11NPQTNSIDD16 pKa = 2.81ITTFEE21 pKa = 4.2NYY23 pKa = 9.44ILRR26 pKa = 11.84IDD28 pKa = 3.94CNKK31 pKa = 9.77VEE33 pKa = 5.05DD34 pKa = 4.32GLRR37 pKa = 11.84TTPCSQNSLNALAIDD52 pKa = 4.18EE53 pKa = 4.41PLEE56 pKa = 4.03YY57 pKa = 10.67ARR59 pKa = 11.84LALDD63 pKa = 3.99GEE65 pKa = 4.4MQAWVDD71 pKa = 4.36AIDD74 pKa = 4.47SLEE77 pKa = 3.86VWW79 pKa = 3.62

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7HCK8|R7HCK8_9FIRM Fe2+/Zn2+ uptake regulation protein OS=Eubacterium sp. CAG:38 OX=1262889 GN=BN634_00437 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.43TRR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.26VHH16 pKa = 5.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2436

0

2436

802892

29

2399

329.6

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.957 ± 0.058

1.498 ± 0.024

5.667 ± 0.039

7.333 ± 0.051

3.982 ± 0.038

6.854 ± 0.049

1.703 ± 0.023

7.394 ± 0.054

7.023 ± 0.043

8.521 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.096 ± 0.029

4.641 ± 0.033

2.974 ± 0.028

3.54 ± 0.033

3.928 ± 0.033

5.755 ± 0.048

5.82 ± 0.053

7.205 ± 0.042

0.772 ± 0.017

4.334 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski