Candidatus Brocadia fulgida

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Candidatus Brocadiae; Candidatus Brocadiales; Candidatus Brocadiaceae; Candidatus Brocadia

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3391 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2UYY5|A0A0M2UYY5_9BACT Cysteine synthase OS=Candidatus Brocadia fulgida OX=380242 GN=BROFUL_01621 PE=4 SV=1
MM1 pKa = 7.27EE2 pKa = 4.93RR3 pKa = 11.84RR4 pKa = 11.84LRR6 pKa = 11.84GHH8 pKa = 6.18EE9 pKa = 4.0PSWWDD14 pKa = 3.53DD15 pKa = 3.56MGLAWYY21 pKa = 10.58NEE23 pKa = 4.28IANTAHH29 pKa = 6.41SGAYY33 pKa = 9.37AQDD36 pKa = 3.38GQTIDD41 pKa = 3.77GNVVDD46 pKa = 5.93SLFTDD51 pKa = 3.65TGLVNWGNDD60 pKa = 3.25HH61 pKa = 7.0NNIDD65 pKa = 3.75VADD68 pKa = 3.91AALIFMHH75 pKa = 6.92GSEE78 pKa = 5.65SNDD81 pKa = 2.49RR82 pKa = 11.84WQGSVRR88 pKa = 11.84VDD90 pKa = 3.06EE91 pKa = 5.64AGAGDD96 pKa = 4.12CQTWQGDD103 pKa = 3.58MRR105 pKa = 11.84FGNTNLKK112 pKa = 9.82FLHH115 pKa = 6.54LSSCNSMDD123 pKa = 3.94DD124 pKa = 3.93NQWADD129 pKa = 3.47RR130 pKa = 11.84WWEE133 pKa = 4.2SFSGLHH139 pKa = 5.48QVDD142 pKa = 4.42GFHH145 pKa = 6.58GFMWIGSDD153 pKa = 4.25LVSDD157 pKa = 4.39YY158 pKa = 11.71EE159 pKa = 4.91DD160 pKa = 3.69FASDD164 pKa = 4.14AFTATIADD172 pKa = 3.26AWLDD176 pKa = 3.7NMYY179 pKa = 10.35HH180 pKa = 6.77ADD182 pKa = 3.57ISGTDD187 pKa = 3.61DD188 pKa = 3.41QCPVAYY194 pKa = 9.87AVGVNSDD201 pKa = 3.89DD202 pKa = 2.93TWNRR206 pKa = 11.84IGAEE210 pKa = 3.93RR211 pKa = 11.84YY212 pKa = 10.41NNVLSNPTSVGYY224 pKa = 9.64WGTIYY229 pKa = 10.81FEE231 pKa = 4.56NCDD234 pKa = 3.9PANEE238 pKa = 4.14DD239 pKa = 4.05TIGTGIDD246 pKa = 3.1

Molecular weight: 27.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2UV12|A0A0M2UV12_9BACT FAD:protein FMN transferase OS=Candidatus Brocadia fulgida OX=380242 GN=BROFUL_02986 PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.57LKK5 pKa = 8.68THH7 pKa = 6.7KK8 pKa = 10.27GLKK11 pKa = 9.64KK12 pKa = 10.16RR13 pKa = 11.84IKK15 pKa = 9.83ISAKK19 pKa = 10.35GKK21 pKa = 8.73VKK23 pKa = 10.4RR24 pKa = 11.84PKK26 pKa = 10.06AGKK29 pKa = 8.71GHH31 pKa = 6.81LMSGKK36 pKa = 8.99SGRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84AHH43 pKa = 5.61LRR45 pKa = 11.84KK46 pKa = 8.78KK47 pKa = 8.97TGVSSAFNKK56 pKa = 9.56TMTRR60 pKa = 11.84ALRR63 pKa = 11.84GSS65 pKa = 3.44

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3391

0

3391

980976

20

2423

289.3

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.231 ± 0.04

1.381 ± 0.016

5.259 ± 0.033

6.675 ± 0.047

4.466 ± 0.034

6.99 ± 0.043

2.275 ± 0.022

7.631 ± 0.037

6.962 ± 0.045

9.336 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.022

4.131 ± 0.03

4.041 ± 0.026

3.228 ± 0.024

5.353 ± 0.036

5.94 ± 0.036

5.39 ± 0.04

6.738 ± 0.033

1.085 ± 0.018

3.399 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski