Devosia sp. Leaf64

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Devosiaceae; Devosia; unclassified Devosia

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4P102|A0A0Q4P102_9RHIZ Probable potassium transport system protein kup OS=Devosia sp. Leaf64 OX=1736229 GN=trkD PE=3 SV=1
MM1 pKa = 7.55AKK3 pKa = 10.26DD4 pKa = 3.31KK5 pKa = 10.53TVAAGDD11 pKa = 3.55LSGWIGTWSGGPEE24 pKa = 3.83QEE26 pKa = 3.93ITITPGANGGLAVEE40 pKa = 5.93GYY42 pKa = 8.38ATYY45 pKa = 10.27GAEE48 pKa = 4.34DD49 pKa = 4.26PEE51 pKa = 4.51SAAMGAINVGGFAGNIPADD70 pKa = 3.88WIEE73 pKa = 4.59DD74 pKa = 3.73NNVTIASTGDD84 pKa = 3.48EE85 pKa = 4.58IIPTSEE91 pKa = 3.99AGHH94 pKa = 5.82YY95 pKa = 9.57DD96 pKa = 3.18CWVDD100 pKa = 3.21MTLNGDD106 pKa = 3.77ALEE109 pKa = 4.35VDD111 pKa = 5.46DD112 pKa = 5.99NGQCGGLNVSFIGTYY127 pKa = 9.49KK128 pKa = 10.32RR129 pKa = 11.84HH130 pKa = 5.38

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4PI64|A0A0Q4PI64_9RHIZ Uncharacterized protein OS=Devosia sp. Leaf64 OX=1736229 GN=ASE94_00180 PE=4 SV=1
MM1 pKa = 7.25GVMKK5 pKa = 10.63KK6 pKa = 8.13MVFAAVVSAIAVFGTVDD23 pKa = 2.84AAFANRR29 pKa = 11.84GGQGFVQEE37 pKa = 4.67RR38 pKa = 11.84VSSRR42 pKa = 11.84GPASFQAFCAFNPHH56 pKa = 5.83EE57 pKa = 4.65CQPGGASKK65 pKa = 10.47IALDD69 pKa = 3.64QSIMRR74 pKa = 11.84ILTRR78 pKa = 11.84VNSRR82 pKa = 11.84VNRR85 pKa = 11.84SIRR88 pKa = 11.84PKK90 pKa = 10.46LDD92 pKa = 3.27NPLVQVWRR100 pKa = 11.84VNPSSGDD107 pKa = 3.67CKK109 pKa = 10.82SYY111 pKa = 10.79AISKK115 pKa = 8.87RR116 pKa = 11.84HH117 pKa = 4.5EE118 pKa = 4.77LIRR121 pKa = 11.84AGLPASALRR130 pKa = 11.84IAYY133 pKa = 8.89VKK135 pKa = 9.23TRR137 pKa = 11.84QGLGHH142 pKa = 6.86AVLVVKK148 pKa = 9.7TDD150 pKa = 3.47QGDD153 pKa = 3.67LTLDD157 pKa = 3.52NLVGEE162 pKa = 4.77IRR164 pKa = 11.84PFHH167 pKa = 5.05QAGYY171 pKa = 9.78RR172 pKa = 11.84VVAMSGADD180 pKa = 3.44PRR182 pKa = 11.84RR183 pKa = 11.84WSS185 pKa = 3.18

Molecular weight: 20.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3949

0

3949

1229070

41

3988

311.2

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.964 ± 0.054

0.643 ± 0.011

5.841 ± 0.032

5.718 ± 0.039

3.906 ± 0.026

8.401 ± 0.046

1.958 ± 0.019

5.621 ± 0.028

3.381 ± 0.029

10.2 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.018

2.911 ± 0.031

4.962 ± 0.037

3.261 ± 0.025

6.22 ± 0.039

5.594 ± 0.034

5.707 ± 0.038

7.543 ± 0.03

1.328 ± 0.017

2.299 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski