Paenibacillus darwinianus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A015LVI9|A0A015LVI9_9BACL Hydrolase OS=Paenibacillus darwinianus OX=1380763 GN=BG52_02210 PE=3 SV=1
MM1 pKa = 7.66EE2 pKa = 4.65EE3 pKa = 3.97HH4 pKa = 6.53TVCPWCDD11 pKa = 3.33TEE13 pKa = 4.97IVWDD17 pKa = 3.95EE18 pKa = 3.84EE19 pKa = 4.36LGPEE23 pKa = 4.93EE24 pKa = 4.3YY25 pKa = 10.42CPHH28 pKa = 7.05CEE30 pKa = 4.17NDD32 pKa = 2.95LKK34 pKa = 11.05AYY36 pKa = 7.64RR37 pKa = 11.84TVSFSIDD44 pKa = 3.52EE45 pKa = 4.19EE46 pKa = 5.29AGAADD51 pKa = 3.72GAGGLRR57 pKa = 11.84EE58 pKa = 4.1TTQAADD64 pKa = 3.51GGIGGG69 pKa = 4.27

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A015M5V6|A0A015M5V6_9BACL Redox-sensing transcriptional repressor Rex OS=Paenibacillus darwinianus OX=1380763 GN=rex PE=3 SV=1
MM1 pKa = 7.02AWRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84CSASNRR13 pKa = 11.84RR14 pKa = 11.84QLPSRR19 pKa = 11.84TRR21 pKa = 11.84RR22 pKa = 11.84PKK24 pKa = 10.35PRR26 pKa = 11.84FTSGWRR32 pKa = 11.84RR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84PRR39 pKa = 11.84CPARR43 pKa = 11.84RR44 pKa = 11.84QPEE47 pKa = 4.15RR48 pKa = 11.84SRR50 pKa = 11.84SGCPGVIRR58 pKa = 11.84CRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84PSSQRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 3.49

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2764

0

2764

836629

37

2415

302.7

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.782 ± 0.058

0.767 ± 0.013

5.239 ± 0.036

6.806 ± 0.055

3.903 ± 0.034

8.297 ± 0.046

1.957 ± 0.026

6.064 ± 0.04

4.742 ± 0.045

9.935 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.875 ± 0.024

3.26 ± 0.033

4.273 ± 0.028

3.422 ± 0.03

6.156 ± 0.046

5.54 ± 0.03

5.151 ± 0.033

7.515 ± 0.038

1.214 ± 0.019

3.102 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski