Cymbidium mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|O10690|O10690_9VIRU Movement protein TGBp3 OS=Cymbidium mosaic virus OX=12178 GN=TGB 2 PE=3 SV=1
MM1 pKa = 7.36EE2 pKa = 5.28LAYY5 pKa = 10.42LVRR8 pKa = 11.84LLEE11 pKa = 4.26HH12 pKa = 6.58NKK14 pKa = 9.78FEE16 pKa = 4.37RR17 pKa = 11.84TNLPLSSPLVVHH29 pKa = 7.05GIAGSGKK36 pKa = 8.17STILTTFHH44 pKa = 6.21HH45 pKa = 7.35HH46 pKa = 5.79YY47 pKa = 8.85PAYY50 pKa = 9.7PIFSHH55 pKa = 6.81SPTLLDD61 pKa = 3.67PSNRR65 pKa = 11.84IYY67 pKa = 9.56QQCITTDD74 pKa = 3.4WVPGGAIVDD83 pKa = 4.0EE84 pKa = 4.58YY85 pKa = 11.45NYY87 pKa = 10.79KK88 pKa = 10.48ALDD91 pKa = 3.6YY92 pKa = 10.45SRR94 pKa = 11.84CLAVFGDD101 pKa = 4.54PLQLPHH107 pKa = 6.95SLQPHH112 pKa = 6.27YY113 pKa = 10.6YY114 pKa = 8.96SSRR117 pKa = 11.84THH119 pKa = 7.06RR120 pKa = 11.84YY121 pKa = 8.48GPKK124 pKa = 9.2LTSLLNDD131 pKa = 4.44LFHH134 pKa = 8.33LSITSLAPVDD144 pKa = 3.99SLDD147 pKa = 3.63YY148 pKa = 10.98ADD150 pKa = 5.34PFAVDD155 pKa = 3.51PSGFTIADD163 pKa = 3.41EE164 pKa = 4.0EE165 pKa = 5.15VYY167 pKa = 11.03NFVSQQVPGTLLPLDD182 pKa = 4.12TVGLEE187 pKa = 3.95YY188 pKa = 10.93SSVSFYY194 pKa = 11.15CSDD197 pKa = 3.31LRR199 pKa = 11.84SCVVLRR205 pKa = 11.84PLSSLHH211 pKa = 6.71RR212 pKa = 11.84SHH214 pKa = 7.49PRR216 pKa = 11.84QGQPHH221 pKa = 7.12HH222 pKa = 7.04LRR224 pKa = 11.84FQCQVV229 pKa = 2.7

Molecular weight:
25.8 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q76SQ1|Q76SQ1_9VIRU Movement protein 2 OS=Cymbidium mosaic virus OX=12178 GN=MP2 PE=4 SV=1
MM1 pKa = 7.74PGLVPPPDD9 pKa = 3.43HH10 pKa = 6.93SKK12 pKa = 11.15SLFVLAIGITVVSALFVLKK31 pKa = 10.73SHH33 pKa = 6.93TFPIAGDD40 pKa = 3.82NIHH43 pKa = 7.03RR44 pKa = 11.84FPSGGQYY51 pKa = 10.64KK52 pKa = 10.81DD53 pKa = 3.02GTKK56 pKa = 10.14QINYY60 pKa = 8.75CPPTHH65 pKa = 6.6ARR67 pKa = 11.84YY68 pKa = 8.59PKK70 pKa = 9.87YY71 pKa = 10.04PDD73 pKa = 3.92YY74 pKa = 11.18KK75 pKa = 10.15WLAATAAIVIPLCLYY90 pKa = 10.44ISYY93 pKa = 10.51HH94 pKa = 6.14PGNNIRR100 pKa = 11.84RR101 pKa = 11.84ICPCCNTYY109 pKa = 10.14HH110 pKa = 6.8HH111 pKa = 7.31PP112 pKa = 3.99

Molecular weight:
12.47 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2072

91

1417

414.4

46.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.784 ± 2.246

1.882 ± 0.413

5.647 ± 0.631

4.778 ± 1.307

4.102 ± 0.141

4.44 ± 0.449

3.571 ± 0.736

6.129 ± 1.021

5.598 ± 1.42

9.99 ± 0.841

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.641 ± 0.492

4.344 ± 0.307

7.432 ± 1.184

3.62 ± 0.568

4.392 ± 0.284

6.419 ± 0.972

6.998 ± 0.53

5.261 ± 0.314

1.11 ± 0.168

3.861 ± 0.66

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski