Mycobacterium phage AlleyCat

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221J7D0|A0A221J7D0_9CAUD Uncharacterized protein OS=Mycobacterium phage AlleyCat OX=2015840 GN=99 PE=4 SV=1
MM1 pKa = 7.76VEE3 pKa = 4.55HH4 pKa = 7.34LDD6 pKa = 4.31DD7 pKa = 3.98NHH9 pKa = 7.49DD10 pKa = 3.41VDD12 pKa = 4.0TDD14 pKa = 3.67HH15 pKa = 8.22AEE17 pKa = 4.24VPVSDD22 pKa = 3.66ITADD26 pKa = 4.05AITGAQQPTTTTPRR40 pKa = 11.84VKK42 pKa = 9.73GTCSNPDD49 pKa = 3.12WTPTVYY55 pKa = 10.36EE56 pKa = 4.44PYY58 pKa = 10.44RR59 pKa = 11.84PP60 pKa = 3.55

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221J776|A0A221J776_9CAUD Uncharacterized protein OS=Mycobacterium phage AlleyCat OX=2015840 GN=48 PE=4 SV=1
MM1 pKa = 7.29AAKK4 pKa = 10.35DD5 pKa = 3.71AVCWTTCPHH14 pKa = 6.1CGKK17 pKa = 10.32RR18 pKa = 11.84SYY20 pKa = 10.49TSRR23 pKa = 11.84KK24 pKa = 7.47DD25 pKa = 3.07AKK27 pKa = 10.03RR28 pKa = 11.84AVSRR32 pKa = 11.84FRR34 pKa = 11.84GDD36 pKa = 2.77RR37 pKa = 11.84GGHH40 pKa = 4.46MSPYY44 pKa = 9.32RR45 pKa = 11.84CHH47 pKa = 7.13DD48 pKa = 3.99DD49 pKa = 3.14EE50 pKa = 5.03SRR52 pKa = 11.84WHH54 pKa = 6.21VGHH57 pKa = 7.36LHH59 pKa = 5.18TATRR63 pKa = 11.84RR64 pKa = 11.84GIHH67 pKa = 5.05YY68 pKa = 7.22EE69 pKa = 3.61QRR71 pKa = 11.84ARR73 pKa = 11.84QQ74 pKa = 3.33

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

19419

41

1370

196.2

21.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.704 ± 0.441

1.056 ± 0.126

6.792 ± 0.206

5.505 ± 0.267

2.616 ± 0.126

8.744 ± 0.406

2.333 ± 0.159

4.032 ± 0.161

3.358 ± 0.19

7.884 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.125

2.894 ± 0.189

5.531 ± 0.223

3.491 ± 0.16

7.642 ± 0.353

4.954 ± 0.213

6.416 ± 0.235

7.73 ± 0.236

1.998 ± 0.122

2.189 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski