Glycocaulis alkaliphilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Maricaulales; Maricaulaceae; Glycocaulis

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0E7B5|A0A3T0E7B5_9PROT UDP-glucose 6-dehydrogenase OS=Glycocaulis alkaliphilus OX=1434191 GN=X907_0549 PE=3 SV=1
MM1 pKa = 7.64SSIAVLAGAASAQEE15 pKa = 4.39GPFRR19 pKa = 11.84LGAGYY24 pKa = 9.47QAIDD28 pKa = 3.81TDD30 pKa = 3.77GATYY34 pKa = 7.78DD35 pKa = 3.64TLTLRR40 pKa = 11.84GSYY43 pKa = 10.32DD44 pKa = 2.99ITPIFSVEE52 pKa = 3.57GDD54 pKa = 3.88LLFGLGDD61 pKa = 3.48EE62 pKa = 4.62STTFGATTITSEE74 pKa = 3.27IDD76 pKa = 3.39YY77 pKa = 10.94GVGIYY82 pKa = 10.31GVARR86 pKa = 11.84LPLNDD91 pKa = 3.48QFSVFARR98 pKa = 11.84GGYY101 pKa = 8.66TYY103 pKa = 11.03FDD105 pKa = 3.83VSASAAGIAFSGDD118 pKa = 2.71VDD120 pKa = 3.48GWAFGGGAEE129 pKa = 4.23WAFAGPNAIRR139 pKa = 11.84VDD141 pKa = 3.4YY142 pKa = 10.16TRR144 pKa = 11.84YY145 pKa = 10.95DD146 pKa = 3.57FVNGNGNADD155 pKa = 3.53AFGISYY161 pKa = 9.79VRR163 pKa = 11.84RR164 pKa = 11.84FF165 pKa = 3.24

Molecular weight: 17.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0E9S2|A0A3T0E9S2_9PROT 3 4-dihydroxy-2-butanone 4-phosphate synthase OS=Glycocaulis alkaliphilus OX=1434191 GN=ribB PE=3 SV=1
MM1 pKa = 7.44LRR3 pKa = 11.84TVLPLALLRR12 pKa = 11.84APLRR16 pKa = 11.84TALRR20 pKa = 11.84TVLLRR25 pKa = 11.84AVLPLALRR33 pKa = 11.84LALRR37 pKa = 11.84TALRR41 pKa = 11.84ALLLLRR47 pKa = 11.84LPAILPVAAVRR58 pKa = 11.84PALRR62 pKa = 4.51

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2866

0

2866

900943

37

2135

314.4

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.578 ± 0.073

0.729 ± 0.015

5.856 ± 0.031

6.335 ± 0.037

3.71 ± 0.028

8.986 ± 0.056

1.955 ± 0.023

4.897 ± 0.03

2.607 ± 0.049

9.976 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.023

2.487 ± 0.029

5.085 ± 0.034

2.941 ± 0.026

7.482 ± 0.045

5.459 ± 0.034

5.019 ± 0.034

7.011 ± 0.035

1.333 ± 0.018

2.128 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski