Enterococcus phage vB_EfaS-DELF1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S9MQ42|A0A5S9MQ42_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS-DELF1 OX=2683673 PE=4 SV=1
MM1 pKa = 7.02MKK3 pKa = 9.97IVVTNYY9 pKa = 10.37NGGEE13 pKa = 4.42GIQVMEE19 pKa = 4.03YY20 pKa = 11.01NYY22 pKa = 8.45ITKK25 pKa = 10.03YY26 pKa = 9.59WFSNDD31 pKa = 3.41YY32 pKa = 10.96LILEE36 pKa = 4.81DD37 pKa = 4.52NVNQIKK43 pKa = 9.28TYY45 pKa = 9.53IQNDD49 pKa = 3.29QVLEE53 pKa = 4.06FEE55 pKa = 4.98VYY57 pKa = 10.42YY58 pKa = 10.53EE59 pKa = 4.0

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S9MR39|A0A5S9MR39_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS-DELF1 OX=2683673 PE=4 SV=1
MM1 pKa = 7.49NYY3 pKa = 9.88IKK5 pKa = 10.97GIMNAIKK12 pKa = 9.48MNRR15 pKa = 11.84VVLYY19 pKa = 10.5KK20 pKa = 10.42LIPSLLLVFSLSYY33 pKa = 10.59LPHH36 pKa = 6.09TVLVYY41 pKa = 10.12IIRR44 pKa = 11.84VTTVGFALMSCGTLIYY60 pKa = 10.42IGVNDD65 pKa = 5.33AKK67 pKa = 10.89FKK69 pKa = 10.28NNKK72 pKa = 8.65KK73 pKa = 10.19KK74 pKa = 10.69RR75 pKa = 11.84INKK78 pKa = 9.07

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12168

55

1441

196.3

22.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.556 ± 0.478

0.575 ± 0.108

6.024 ± 0.285

7.52 ± 0.439

4.076 ± 0.224

6.542 ± 0.528

1.479 ± 0.159

6.616 ± 0.227

8.843 ± 0.372

8.695 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.622 ± 0.12

6.977 ± 0.32

2.901 ± 0.188

3.567 ± 0.189

3.838 ± 0.217

5.531 ± 0.286

6.402 ± 0.347

6.55 ± 0.239

1.233 ± 0.098

4.454 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski