Clostridium sp. CAG:762

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5ST25|R5ST25_9CLOT Cell shape-determining protein MreB OS=Clostridium sp. CAG:762 OX=1262837 GN=mreB PE=3 SV=1
MM1 pKa = 7.4IRR3 pKa = 11.84TEE5 pKa = 3.99TDD7 pKa = 2.59EE8 pKa = 6.07DD9 pKa = 3.73ITDD12 pKa = 3.45EE13 pKa = 4.4EE14 pKa = 4.84YY15 pKa = 10.63IKK17 pKa = 10.72VLKK20 pKa = 10.52PFVDD24 pKa = 4.11NYY26 pKa = 11.53DD27 pKa = 3.94EE28 pKa = 4.32YY29 pKa = 11.63LEE31 pKa = 5.31SYY33 pKa = 9.91VLPEE37 pKa = 5.37LISNYY42 pKa = 9.23IANAA46 pKa = 3.62

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5TDS3|R5TDS3_9CLOT Uncharacterized conserved protein OS=Clostridium sp. CAG:762 OX=1262837 GN=BN775_00625 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84KK3 pKa = 9.55LNKK6 pKa = 10.02NEE8 pKa = 4.74LININGGFKK17 pKa = 10.22ISGAILQYY25 pKa = 11.07GRR27 pKa = 11.84LFVLAFYY34 pKa = 10.51DD35 pKa = 3.11IGVGVGSAIRR45 pKa = 11.84RR46 pKa = 11.84FSKK49 pKa = 10.62RR50 pKa = 11.84NYY52 pKa = 10.16CPINN56 pKa = 3.4

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1127

0

1127

327007

30

1441

290.2

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.02 ± 0.059

1.043 ± 0.029

5.945 ± 0.054

6.881 ± 0.075

4.075 ± 0.059

5.215 ± 0.069

1.324 ± 0.027

10.738 ± 0.097

10.183 ± 0.074

9.167 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.03

8.198 ± 0.087

2.408 ± 0.036

1.971 ± 0.036

2.989 ± 0.043

6.035 ± 0.051

5.478 ± 0.061

5.897 ± 0.065

0.543 ± 0.021

5.517 ± 0.076
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski