Herbaspirillum sp. K1R23-30

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Herbaspirillum; unclassified Herbaspirillum

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A3FT85|A0A3A3FT85_9BURK 3-hydroxyacyl-CoA dehydrogenase OS=Herbaspirillum sp. K1R23-30 OX=2320863 GN=D3871_03555 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 9.92FDD4 pKa = 3.74TAAPEE9 pKa = 4.03ATYY12 pKa = 9.98EE13 pKa = 4.19PPLSAIEE20 pKa = 3.99TSNTYY25 pKa = 9.92VVATGDD31 pKa = 3.68VLSQLSSFAKK41 pKa = 10.56LEE43 pKa = 4.46PGWDD47 pKa = 3.76GADD50 pKa = 3.24SVVPASQDD58 pKa = 2.67IEE60 pKa = 4.09AALDD64 pKa = 3.94FVLSMPPVLPLPKK77 pKa = 10.57AMLSASGEE85 pKa = 4.1LGLYY89 pKa = 9.22WDD91 pKa = 5.51DD92 pKa = 3.39NDD94 pKa = 4.09IYY96 pKa = 10.77IDD98 pKa = 3.34IAFEE102 pKa = 4.15PEE104 pKa = 4.13GKK106 pKa = 9.86ISIYY110 pKa = 10.81SKK112 pKa = 10.1IRR114 pKa = 11.84STGKK118 pKa = 9.88EE119 pKa = 3.69KK120 pKa = 10.51FYY122 pKa = 11.26DD123 pKa = 4.25SIDD126 pKa = 3.59TTSINSNWYY135 pKa = 9.96FDD137 pKa = 3.77TLGDD141 pKa = 3.72LLIPHH146 pKa = 7.45GYY148 pKa = 10.52ALAAA152 pKa = 4.28

Molecular weight: 16.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A3FQ26|A0A3A3FQ26_9BURK IS630 family transposase OS=Herbaspirillum sp. K1R23-30 OX=2320863 GN=D3871_19400 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5690

0

5690

1844030

24

2230

324.1

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.534 ± 0.04

0.978 ± 0.011

5.348 ± 0.022

5.551 ± 0.027

3.69 ± 0.019

7.858 ± 0.033

2.281 ± 0.016

5.331 ± 0.022

4.033 ± 0.03

10.111 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.721 ± 0.015

3.219 ± 0.018

4.788 ± 0.024

3.862 ± 0.021

6.379 ± 0.031

5.855 ± 0.023

5.328 ± 0.026

7.33 ± 0.024

1.279 ± 0.011

2.525 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski