Prevotella sp. CAG:1124

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2441 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5KZL9|R5KZL9_9BACT GHMP kinase protein OS=Prevotella sp. CAG:1124 OX=1262920 GN=BN467_00759 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.51KK3 pKa = 10.54LFLTAMAFVAMMSAMSFTSCSDD25 pKa = 4.06DD26 pKa = 3.45DD27 pKa = 4.28TNDD30 pKa = 3.28NGGGGGGGSTTGGVEE45 pKa = 3.92LNGAIEE51 pKa = 4.32GTVTLEE57 pKa = 3.42ADD59 pKa = 3.06KK60 pKa = 10.68EE61 pKa = 4.17YY62 pKa = 10.97RR63 pKa = 11.84LTGAVTVPDD72 pKa = 4.31GATLNIPAGTTIRR85 pKa = 11.84ATQGFSSYY93 pKa = 11.04ILVEE97 pKa = 4.05RR98 pKa = 11.84GGTINAEE105 pKa = 4.03GTADD109 pKa = 3.52KK110 pKa = 10.57PIVFTADD117 pKa = 3.01TDD119 pKa = 3.82NATAGYY125 pKa = 8.74WGGLIINGNAPITGGGEE142 pKa = 4.19GVTEE146 pKa = 3.72IDD148 pKa = 3.56NNVSYY153 pKa = 11.02GGNDD157 pKa = 3.32ANDD160 pKa = 3.25NSGVIRR166 pKa = 11.84YY167 pKa = 9.18VEE169 pKa = 4.08LWYY172 pKa = 9.77TGARR176 pKa = 11.84SSADD180 pKa = 3.05IEE182 pKa = 4.94HH183 pKa = 7.07NGLTLNGVGNGTTIEE198 pKa = 4.05NVYY201 pKa = 9.94IAEE204 pKa = 4.43GADD207 pKa = 3.41DD208 pKa = 4.88GIEE211 pKa = 4.19FFGGTVNVSNLLVVNCDD228 pKa = 3.5DD229 pKa = 4.56DD230 pKa = 6.25CFDD233 pKa = 3.77FTQGYY238 pKa = 10.06SGTLTNCYY246 pKa = 10.0GIWEE250 pKa = 4.92DD251 pKa = 4.19GFTSTEE257 pKa = 3.67EE258 pKa = 3.97DD259 pKa = 3.46PRR261 pKa = 11.84GVEE264 pKa = 4.42ADD266 pKa = 3.64GNLDD270 pKa = 3.05GDD272 pKa = 4.4MPDD275 pKa = 4.42GAPQADD281 pKa = 3.85FTINGMTIVNNSSTQSMQDD300 pKa = 3.15AIKK303 pKa = 10.35VRR305 pKa = 11.84RR306 pKa = 11.84GATAHH311 pKa = 6.01ITDD314 pKa = 3.54AVVYY318 pKa = 10.33GSGQIEE324 pKa = 4.23NLIDD328 pKa = 3.56LTDD331 pKa = 3.78GKK333 pKa = 10.91GGAGRR338 pKa = 11.84STDD341 pKa = 3.09MSVSNNAYY349 pKa = 9.59YY350 pKa = 10.18ISDD353 pKa = 3.33ATINDD358 pKa = 3.49GGTTYY363 pKa = 10.88SGVNVEE369 pKa = 4.56TGNDD373 pKa = 4.0GADD376 pKa = 3.23VSALSWTGYY385 pKa = 9.29EE386 pKa = 4.96FPQLNNTTVAAAGDD400 pKa = 4.07LPLEE404 pKa = 4.3ISSPIALEE412 pKa = 4.04AGQEE416 pKa = 4.06YY417 pKa = 9.91FINGSVHH424 pKa = 5.15VKK426 pKa = 10.24EE427 pKa = 4.91GGVLLIPAGMTIKK440 pKa = 10.54ARR442 pKa = 11.84EE443 pKa = 4.13GFSNFILVEE452 pKa = 4.15RR453 pKa = 11.84GGKK456 pKa = 9.36IYY458 pKa = 9.88ATGRR462 pKa = 11.84EE463 pKa = 4.11DD464 pKa = 4.5APITFTADD472 pKa = 2.8ASNATAGYY480 pKa = 8.41WGGIIINGNAVISGPSGSVNEE501 pKa = 4.63GVTEE505 pKa = 3.76IDD507 pKa = 3.23NNVPYY512 pKa = 10.9GGDD515 pKa = 3.39QNNDD519 pKa = 2.72NSGVLTYY526 pKa = 10.55VSILYY531 pKa = 10.48SGARR535 pKa = 11.84SSADD539 pKa = 3.0IEE541 pKa = 4.94HH542 pKa = 7.07NGLTLNGVGAGTTIEE557 pKa = 4.38NIYY560 pKa = 9.78IAEE563 pKa = 4.37GADD566 pKa = 3.45DD567 pKa = 4.88GIEE570 pKa = 4.08FFGGSVNVSNLLVVNCDD587 pKa = 3.5DD588 pKa = 4.56DD589 pKa = 6.25CFDD592 pKa = 3.77FTQGYY597 pKa = 10.06SGTLTNCYY605 pKa = 9.78GRR607 pKa = 11.84WEE609 pKa = 4.34AGFTSTEE616 pKa = 3.72LDD618 pKa = 3.47PRR620 pKa = 11.84GVEE623 pKa = 4.22ADD625 pKa = 3.62GNLDD629 pKa = 3.79GEE631 pKa = 4.97GSTAHH636 pKa = 6.59SPQSDD641 pKa = 3.55FTIEE645 pKa = 3.99NMTIEE650 pKa = 4.47NLSSYY655 pKa = 10.44PMQDD659 pKa = 2.97AIKK662 pKa = 10.34VRR664 pKa = 11.84RR665 pKa = 11.84LAKK668 pKa = 9.66ATINNALVIGSGEE681 pKa = 3.86IQEE684 pKa = 5.51LVDD687 pKa = 4.19LTDD690 pKa = 4.42GLDD693 pKa = 3.92DD694 pKa = 5.39ADD696 pKa = 3.91PTSVINVTNGATNVGAQTNSSNPEE720 pKa = 3.65GATVNIADD728 pKa = 4.43GNTGCEE734 pKa = 3.58TDD736 pKa = 2.87IFGWTGYY743 pKa = 7.51TLL745 pKa = 3.63

Molecular weight: 77.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5KK40|R5KK40_9BACT Uncharacterized protein OS=Prevotella sp. CAG:1124 OX=1262920 GN=BN467_02137 PE=4 SV=1
MM1 pKa = 7.73PMLIVLATTTLGRR14 pKa = 11.84WRR16 pKa = 11.84SHH18 pKa = 5.57RR19 pKa = 11.84RR20 pKa = 11.84PLTRR24 pKa = 11.84LPATMPKK31 pKa = 9.24PAAIIKK37 pKa = 10.28AGSTSAAKK45 pKa = 10.01PPSVVSKK52 pKa = 11.33GLMM55 pKa = 3.3

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2441

0

2441

807868

29

2609

331.0

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.646 ± 0.046

1.408 ± 0.022

6.19 ± 0.044

5.966 ± 0.046

4.29 ± 0.031

7.313 ± 0.044

1.881 ± 0.021

6.541 ± 0.045

6.069 ± 0.05

8.545 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.016 ± 0.028

4.989 ± 0.043

3.708 ± 0.027

2.992 ± 0.028

5.15 ± 0.048

5.91 ± 0.052

5.886 ± 0.045

6.954 ± 0.045

1.212 ± 0.019

4.332 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski