Streptomyces sp. Ru73

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S4Y1R8|A0A2S4Y1R8_9ACTN Epoxide hydrolase OS=Streptomyces sp. Ru73 OX=2080748 GN=C3486_02830 PE=3 SV=1
MM1 pKa = 7.12HH2 pKa = 8.12AYY4 pKa = 7.69RR5 pKa = 11.84TLAATATLALTLGTAALAAPAAQAASAATATVVHH39 pKa = 6.96EE40 pKa = 5.86DD41 pKa = 3.58GEE43 pKa = 4.56LWYY46 pKa = 10.44KK47 pKa = 10.24AAPGQQNQLTVDD59 pKa = 3.6EE60 pKa = 5.21EE61 pKa = 4.19IEE63 pKa = 3.87QRR65 pKa = 11.84GEE67 pKa = 3.79FEE69 pKa = 4.0SYY71 pKa = 10.87YY72 pKa = 11.21VLTFHH77 pKa = 7.36DD78 pKa = 5.01RR79 pKa = 11.84YY80 pKa = 10.93DD81 pKa = 3.32IAIDD85 pKa = 3.57PGAAEE90 pKa = 4.5WDD92 pKa = 3.57EE93 pKa = 4.17CAYY96 pKa = 9.79PAEE99 pKa = 4.52GDD101 pKa = 3.64RR102 pKa = 11.84TVVRR106 pKa = 11.84CAVEE110 pKa = 3.96IPVNSDD116 pKa = 3.3DD117 pKa = 4.44SDD119 pKa = 4.29SYY121 pKa = 11.82DD122 pKa = 4.39IDD124 pKa = 6.05LGDD127 pKa = 4.16QDD129 pKa = 4.57DD130 pKa = 4.32TATLEE135 pKa = 4.36PDD137 pKa = 2.81SDD139 pKa = 3.24AWAGVHH145 pKa = 6.55GGPGNDD151 pKa = 3.27VLKK154 pKa = 10.93GSASAMLYY162 pKa = 10.85GDD164 pKa = 5.39DD165 pKa = 5.02GDD167 pKa = 5.17DD168 pKa = 3.74RR169 pKa = 11.84LDD171 pKa = 3.83GGGGPFGFGSYY182 pKa = 10.17GGPGNDD188 pKa = 3.06VLTHH192 pKa = 6.76CGQDD196 pKa = 3.42CFGGAGNDD204 pKa = 3.63SLTGTDD210 pKa = 4.49EE211 pKa = 4.14EE212 pKa = 4.7NTLHH216 pKa = 7.15GDD218 pKa = 3.52DD219 pKa = 5.04GNDD222 pKa = 3.36VLHH225 pKa = 6.61GRR227 pKa = 11.84GGADD231 pKa = 2.96VLYY234 pKa = 10.41GGKK237 pKa = 10.8GNDD240 pKa = 3.25TLYY243 pKa = 11.27GEE245 pKa = 4.92EE246 pKa = 4.97GNDD249 pKa = 3.77TLWGNSGDD257 pKa = 4.2DD258 pKa = 3.91ILWGGNGTDD267 pKa = 3.93TLSGGPGRR275 pKa = 11.84NQLHH279 pKa = 5.93QDD281 pKa = 2.96

Molecular weight: 29.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S4XWL0|A0A2S4XWL0_9ACTN Sugar ABC transporter permease OS=Streptomyces sp. Ru73 OX=2080748 GN=C3486_11590 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7006

0

7006

2295001

22

3126

327.6

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.185 ± 0.045

0.816 ± 0.007

5.86 ± 0.025

5.68 ± 0.027

2.72 ± 0.016

9.715 ± 0.027

2.385 ± 0.015

3.06 ± 0.02

2.195 ± 0.025

10.351 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.74 ± 0.011

1.661 ± 0.013

6.234 ± 0.024

2.706 ± 0.019

8.175 ± 0.034

4.677 ± 0.017

6.025 ± 0.022

8.246 ± 0.028

1.462 ± 0.013

2.107 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski