Fretibacterium fastidiosum

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Fretibacterium

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4M8K6|D4M8K6_9BACT NCAIR mutase (PurE)-related proteins OS=Fretibacterium fastidiosum OX=651822 GN=SY1_09440 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 5.93WIKK5 pKa = 11.18APADD9 pKa = 3.71TPVCPEE15 pKa = 4.06KK16 pKa = 11.14NILLKK21 pKa = 10.87DD22 pKa = 3.65IVQLILDD29 pKa = 3.98GAEE32 pKa = 3.88TPEE35 pKa = 3.78AVMAEE40 pKa = 4.16LEE42 pKa = 4.33LTGTEE47 pKa = 4.36EE48 pKa = 4.43GTDD51 pKa = 3.76QIPAILDD58 pKa = 3.33VFVPVVNAWQTGICSGGCSGCSGGCCGEE86 pKa = 4.03

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4M877|D4M877_9BACT Archaeal/vacuolar-type H+-ATPase subunit F OS=Fretibacterium fastidiosum OX=651822 GN=SY1_07380 PE=3 SV=1
MM1 pKa = 7.2LAVRR5 pKa = 11.84KK6 pKa = 9.82RR7 pKa = 11.84IPAPRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84ASMRR18 pKa = 11.84AGTNTAKK25 pKa = 10.36VFPTPVGASRR35 pKa = 11.84ARR37 pKa = 11.84TAASASLRR45 pKa = 11.84NPP47 pKa = 3.35

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1434

0

1434

431014

14

1779

300.6

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.543 ± 0.089

1.35 ± 0.027

5.399 ± 0.041

6.669 ± 0.072

3.905 ± 0.045

8.615 ± 0.057

1.659 ± 0.026

4.98 ± 0.061

4.122 ± 0.055

10.546 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.886 ± 0.031

2.854 ± 0.038

4.519 ± 0.047

2.711 ± 0.036

7.109 ± 0.079

5.64 ± 0.047

4.852 ± 0.045

7.77 ± 0.053

1.271 ± 0.027

2.6 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski