Piscine myocarditis-like virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 8.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125SJD2|A0A125SJD2_9VIRU RNA-directed RNA polymerase OS=Piscine myocarditis-like virus OX=1798085 PE=3 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84IAGDD6 pKa = 3.59RR7 pKa = 11.84QTVLLGLPSGKK18 pKa = 10.44GGISVLVGDD27 pKa = 4.92FATNLEE33 pKa = 4.39SVARR37 pKa = 11.84AIFTDD42 pKa = 3.25TNMVVDD48 pKa = 3.85ATIEE52 pKa = 3.82DD53 pKa = 3.73RR54 pKa = 11.84ARR56 pKa = 11.84ARR58 pKa = 11.84NHH60 pKa = 5.73TILRR64 pKa = 11.84EE65 pKa = 3.96GLIDD69 pKa = 3.29SRR71 pKa = 11.84MYY73 pKa = 10.1MSAGRR78 pKa = 11.84TTQVALWAAGPPVAVGVNPTWYY100 pKa = 9.41ILRR103 pKa = 11.84PGKK106 pKa = 10.53VEE108 pKa = 5.32GIMLTQSSSMASLAPLFRR126 pKa = 11.84GVEE129 pKa = 4.07LGPFASAVRR138 pKa = 11.84ADD140 pKa = 3.33VGFYY144 pKa = 11.11SMNVVLGALRR154 pKa = 11.84AAGFNAEE161 pKa = 4.06NGRR164 pKa = 11.84VSFVEE169 pKa = 3.52PLLRR173 pKa = 11.84VQCKK177 pKa = 9.93ILDD180 pKa = 4.03GQDD183 pKa = 3.3RR184 pKa = 11.84GEE186 pKa = 4.13SPYY189 pKa = 10.91SIVGGLTSQLVNPVTQRR206 pKa = 11.84VWGNFFPGGPNQAAWVAADD225 pKa = 3.54TVSRR229 pKa = 11.84VVNIEE234 pKa = 3.59DD235 pKa = 4.31FMRR238 pKa = 11.84EE239 pKa = 3.85AQGEE243 pKa = 4.2NRR245 pKa = 11.84FAAGWGPGFWNKK257 pKa = 9.26MGATGVAVVPIKK269 pKa = 10.24TSGAMLAQQNSAWTLAHH286 pKa = 6.09MEE288 pKa = 4.15YY289 pKa = 9.73PRR291 pKa = 11.84KK292 pKa = 9.76VRR294 pKa = 11.84QVDD297 pKa = 3.69TNVISINAALAAVGAEE313 pKa = 3.66RR314 pKa = 11.84EE315 pKa = 4.29YY316 pKa = 11.22TNTSCTHH323 pKa = 4.78VPGPYY328 pKa = 10.11GKK330 pKa = 10.0VLFVIVDD337 pKa = 3.51QTTDD341 pKa = 2.92RR342 pKa = 11.84AVQVAVEE349 pKa = 4.23GAVGVLLTTPALHH362 pKa = 5.68YY363 pKa = 10.58VIGGIDD369 pKa = 3.4MGIGLMVTRR378 pKa = 11.84EE379 pKa = 3.76VGLAIDD385 pKa = 3.68QAALEE390 pKa = 4.89NIMAMEE396 pKa = 4.43RR397 pKa = 11.84WVEE400 pKa = 4.04FYY402 pKa = 11.31GNEE405 pKa = 4.14EE406 pKa = 4.21DD407 pKa = 3.64WQSATTIVGCAYY419 pKa = 9.21VTFGVEE425 pKa = 4.15MRR427 pKa = 11.84RR428 pKa = 11.84HH429 pKa = 4.81TNRR432 pKa = 11.84VEE434 pKa = 3.95GGYY437 pKa = 9.44YY438 pKa = 8.16WQVGANPPFPLGGLANWTRR457 pKa = 11.84LDD459 pKa = 3.77NLTAAQQAAIYY470 pKa = 10.76ADD472 pKa = 3.14IQNTVEE478 pKa = 4.15APWGGFRR485 pKa = 11.84VRR487 pKa = 11.84NPVPTAAPNAMYY499 pKa = 9.96TRR501 pKa = 11.84FEE503 pKa = 4.06PVLAVGTVAMLWRR516 pKa = 11.84PEE518 pKa = 4.05HH519 pKa = 6.85PMLAPLPDD527 pKa = 4.19RR528 pKa = 11.84PSALADD534 pKa = 2.85ICYY537 pKa = 10.13QRR539 pKa = 11.84GRR541 pKa = 11.84MCAAQEE547 pKa = 4.46DD548 pKa = 4.94IVVGQLGFPEE558 pKa = 4.25DD559 pKa = 4.03MIRR562 pKa = 11.84APVGVGNVGLAQRR575 pKa = 11.84LIRR578 pKa = 11.84KK579 pKa = 6.56MCNVNNQILEE589 pKa = 4.27AKK591 pKa = 9.46HH592 pKa = 6.02ASTVHH597 pKa = 5.22VVLNGYY603 pKa = 8.67WSIGAGVTYY612 pKa = 10.28RR613 pKa = 11.84AIYY616 pKa = 9.01AQSGPGTMRR625 pKa = 11.84KK626 pKa = 9.71RR627 pKa = 11.84IPWAEE632 pKa = 3.87MRR634 pKa = 11.84SLGFDD639 pKa = 3.36ADD641 pKa = 4.05LVKK644 pKa = 10.94NDD646 pKa = 4.2LYY648 pKa = 11.46GIGSGDD654 pKa = 3.46FVAVNATIGALGGIWDD670 pKa = 3.95VVRR673 pKa = 11.84YY674 pKa = 7.15PLPVPSKK681 pKa = 9.12EE682 pKa = 3.71ARR684 pKa = 11.84AAMNTTNCMGGWTRR698 pKa = 11.84AVTRR702 pKa = 11.84VDD704 pKa = 3.05NLGTIRR710 pKa = 11.84EE711 pKa = 4.46GGLEE715 pKa = 4.14PEE717 pKa = 4.21PLGIVMKK724 pKa = 10.32TPTASRR730 pKa = 11.84ATYY733 pKa = 10.23LRR735 pKa = 11.84WSLFATPAIQSDD747 pKa = 3.26TRR749 pKa = 11.84EE750 pKa = 4.14YY751 pKa = 10.64PFGGYY756 pKa = 9.04SRR758 pKa = 11.84SGTEE762 pKa = 4.06TNHH765 pKa = 6.37HH766 pKa = 6.4VLTALSGGRR775 pKa = 11.84IVVLKK780 pKa = 10.63DD781 pKa = 3.28AEE783 pKa = 4.43AEE785 pKa = 4.14QNPVFLNRR793 pKa = 11.84TSGQPTTNTPQVDD806 pKa = 4.14SQGSTTEE813 pKa = 3.75AFLDD817 pKa = 3.61II818 pKa = 5.09

Molecular weight: 88.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125SJD3|A0A125SJD3_9VIRU Structural protein OS=Piscine myocarditis-like virus OX=1798085 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.18GACRR6 pKa = 11.84YY7 pKa = 8.01TMEE10 pKa = 4.24IYY12 pKa = 10.8FNFKK16 pKa = 10.29CSNKK20 pKa = 9.99SLSLMLGFILIMYY33 pKa = 9.13LMSLLQKK40 pKa = 10.78VGAQPLALKK49 pKa = 9.03VTEE52 pKa = 4.78TYY54 pKa = 10.83CPTFLTLKK62 pKa = 10.6VPDD65 pKa = 4.31KK66 pKa = 10.61LISSMKK72 pKa = 9.11TVTGGWIVKK81 pKa = 8.0TAAGRR86 pKa = 11.84EE87 pKa = 4.21ICVHH91 pKa = 5.83GDD93 pKa = 2.2WWEE96 pKa = 3.88AQFNLLGKK104 pKa = 10.41GRR106 pKa = 11.84LEE108 pKa = 4.38RR109 pKa = 11.84ITLHH113 pKa = 4.6GHH115 pKa = 4.97EE116 pKa = 4.61VLVPTKK122 pKa = 10.62FEE124 pKa = 4.05IKK126 pKa = 10.48VNEE129 pKa = 4.19DD130 pKa = 2.92HH131 pKa = 7.77SIVQTNVNCANGIGTWNLFGRR152 pKa = 11.84VDD154 pKa = 3.72CTVVEE159 pKa = 4.41NVVQGHH165 pKa = 6.69DD166 pKa = 3.52NQAAMAAVPPAQPPPPPLQTLWSEE190 pKa = 3.97IVGWGTDD197 pKa = 3.36TLILIGIVLALILIIWKK214 pKa = 9.18GVPCVVRR221 pKa = 11.84LIVKK225 pKa = 9.98RR226 pKa = 11.84KK227 pKa = 9.55KK228 pKa = 10.2KK229 pKa = 10.25NKK231 pKa = 8.38QKK233 pKa = 11.01RR234 pKa = 11.84KK235 pKa = 8.93IKK237 pKa = 10.23KK238 pKa = 9.21KK239 pKa = 10.03KK240 pKa = 9.49KK241 pKa = 9.33KK242 pKa = 9.81KK243 pKa = 9.68KK244 pKa = 9.63KK245 pKa = 9.63KK246 pKa = 9.63KK247 pKa = 9.63KK248 pKa = 9.63KK249 pKa = 9.63KK250 pKa = 9.63KK251 pKa = 9.63KK252 pKa = 9.63KK253 pKa = 9.63KK254 pKa = 9.48KK255 pKa = 9.26KK256 pKa = 9.28KK257 pKa = 9.31KK258 pKa = 9.95KK259 pKa = 8.27KK260 pKa = 7.97TFLIIYY266 pKa = 6.93SQKK269 pKa = 10.53KK270 pKa = 9.03KK271 pKa = 10.46KK272 pKa = 10.43KK273 pKa = 8.48KK274 pKa = 8.29TKK276 pKa = 9.07KK277 pKa = 10.19QNTILHH283 pKa = 6.58LSYY286 pKa = 10.95IKK288 pKa = 10.24IKK290 pKa = 10.83KK291 pKa = 7.53KK292 pKa = 8.44TKK294 pKa = 9.6KK295 pKa = 9.86KK296 pKa = 10.07KK297 pKa = 9.62KK298 pKa = 9.88KK299 pKa = 8.17KK300 pKa = 8.74TKK302 pKa = 10.21

Molecular weight: 34.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1951

302

831

650.3

72.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.381 ± 2.091

1.076 ± 0.245

4.511 ± 0.778

5.331 ± 0.715

3.434 ± 0.365

8.303 ± 1.07

1.23 ± 0.171

5.997 ± 0.668

7.278 ± 3.326

8.303 ± 0.594

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.331

4.664 ± 0.149

4.408 ± 0.721

3.178 ± 0.45

6.509 ± 0.826

5.894 ± 1.276

6.048 ± 0.785

8.662 ± 0.765

2.46 ± 0.183

2.614 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski