Phytophthora rubi

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A4C5U4|A0A6A4C5U4_9STRA Uncharacterized protein OS=Phytophthora rubi OX=129364 GN=PR002_g26572 PE=4 SV=1
MM1 pKa = 7.44TFLILEE7 pKa = 4.28NSSYY11 pKa = 9.77STSSQQPVYY20 pKa = 10.81LCVDD24 pKa = 3.74MNIHH28 pKa = 6.83ALLLVAASMTASATAKK44 pKa = 9.17FTVTSGTTEE53 pKa = 3.83ATTSTTEE60 pKa = 3.39NVAFVKK66 pKa = 10.31QWTLNSATTADD77 pKa = 4.32TIDD80 pKa = 5.43SIDD83 pKa = 4.08LDD85 pKa = 4.04LAGRR89 pKa = 11.84VYY91 pKa = 10.86VSYY94 pKa = 11.41VSGLPSGVLGYY105 pKa = 11.23VNVSGDD111 pKa = 3.4SQTVVDD117 pKa = 5.39AVTVINDD124 pKa = 3.96DD125 pKa = 5.31ANDD128 pKa = 3.77TDD130 pKa = 6.41DD131 pKa = 6.07NDD133 pKa = 4.24TNDD136 pKa = 3.84NDD138 pKa = 3.95GEE140 pKa = 4.59LNVRR144 pKa = 11.84IGNSTSTATTLSGYY158 pKa = 10.66LLTEE162 pKa = 5.02IILASSGVVTDD173 pKa = 3.82VKK175 pKa = 10.3SQRR178 pKa = 11.84SAQVVVEE185 pKa = 4.38DD186 pKa = 4.42GVLVSSSTTAEE197 pKa = 4.15LQVEE201 pKa = 4.55ASGSSAVYY209 pKa = 10.06VSAASTAVSVRR220 pKa = 11.84QLQLDD225 pKa = 3.82AAGTAQLQFNVKK237 pKa = 9.39SVSVTDD243 pKa = 3.57EE244 pKa = 4.16AQFEE248 pKa = 4.51AQGSAVVSVLASSLEE263 pKa = 3.79ASQLDD268 pKa = 4.89LEE270 pKa = 4.65TQSTGTICISAQQVTATNYY289 pKa = 9.59QGQDD293 pKa = 2.86ASRR296 pKa = 11.84ISMPNASSKK305 pKa = 10.83YY306 pKa = 10.21DD307 pKa = 3.49STGSLACDD315 pKa = 3.39EE316 pKa = 4.63SSIPTRR322 pKa = 11.84EE323 pKa = 4.0PSCVSSACASSSTSGTSTTATVVTSAPTTTNDD355 pKa = 4.33DD356 pKa = 4.02DD357 pKa = 6.48ASDD360 pKa = 4.49DD361 pKa = 4.26SNASSTPRR369 pKa = 11.84LAALVVAVIGVVTAGLLL386 pKa = 3.53

Molecular weight: 39.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A4BL47|A0A6A4BL47_9STRA Chromo domain-containing protein OS=Phytophthora rubi OX=129364 GN=PR003_g29016 PE=4 SV=1
MM1 pKa = 7.77SSQKK5 pKa = 10.26KK6 pKa = 10.05SKK8 pKa = 10.72SQMKK12 pKa = 9.72PKK14 pKa = 10.12SQRR17 pKa = 11.84LSSRR21 pKa = 11.84KK22 pKa = 8.32KK23 pKa = 8.07SKK25 pKa = 9.78RR26 pKa = 11.84RR27 pKa = 11.84KK28 pKa = 7.38VSKK31 pKa = 9.92RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 8.37SKK36 pKa = 10.78RR37 pKa = 11.84KK38 pKa = 8.0ISKK41 pKa = 9.53SQVVTT46 pKa = 3.85

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35064

315

35379

12151655

10

10508

343.5

38.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.602 ± 0.015

1.629 ± 0.008

5.819 ± 0.01

6.625 ± 0.015

3.464 ± 0.009

6.205 ± 0.015

2.321 ± 0.006

3.673 ± 0.01

4.909 ± 0.016

9.097 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.395 ± 0.006

3.212 ± 0.008

5.112 ± 0.014

4.157 ± 0.01

6.791 ± 0.016

8.151 ± 0.017

5.907 ± 0.014

7.181 ± 0.014

1.277 ± 0.004

2.472 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski