Gordonia phage Rabbitrun

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LIS1|A0A7G8LIS1_9CAUD Uncharacterized protein OS=Gordonia phage Rabbitrun OX=2762280 GN=110 PE=4 SV=1
MM1 pKa = 7.4TSIQALHH8 pKa = 5.6EE9 pKa = 4.23QQVEE13 pKa = 4.08EE14 pKa = 4.42ARR16 pKa = 11.84RR17 pKa = 11.84EE18 pKa = 4.21AYY20 pKa = 10.21DD21 pKa = 3.17EE22 pKa = 5.27GYY24 pKa = 8.6DD25 pKa = 3.12TGYY28 pKa = 11.62ALGYY32 pKa = 10.34DD33 pKa = 3.67AGFEE37 pKa = 4.53DD38 pKa = 5.59GSEE41 pKa = 4.1APLAA45 pKa = 4.3

Molecular weight: 4.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LIV4|A0A7G8LIV4_9CAUD Uncharacterized protein OS=Gordonia phage Rabbitrun OX=2762280 GN=130 PE=4 SV=1
MM1 pKa = 7.25GHH3 pKa = 6.78LQRR6 pKa = 11.84FCRR9 pKa = 11.84VRR11 pKa = 11.84GFEE14 pKa = 4.03SRR16 pKa = 11.84RR17 pKa = 11.84SDD19 pKa = 2.82GRR21 pKa = 11.84RR22 pKa = 11.84CVDD25 pKa = 3.63CGYY28 pKa = 8.18FTNWICRR35 pKa = 11.84FKK37 pKa = 10.51SCRR40 pKa = 11.84RR41 pKa = 11.84GVKK44 pKa = 10.43LPDD47 pKa = 3.14VCSTIDD53 pKa = 3.4QVHH56 pKa = 6.26SRR58 pKa = 11.84QQPPQMRR65 pKa = 11.84RR66 pKa = 11.84LHH68 pKa = 7.31SYY70 pKa = 8.81FNWMKK75 pKa = 10.04RR76 pKa = 11.84TLCAIQFPGVTSSRR90 pKa = 11.84SDD92 pKa = 2.92AA93 pKa = 3.92

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

132

0

132

23912

22

2228

181.2

19.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.275 ± 0.333

0.924 ± 0.142

6.838 ± 0.21

6.168 ± 0.268

3.459 ± 0.121

8.473 ± 0.358

2.083 ± 0.174

4.517 ± 0.169

4.303 ± 0.201

7.929 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.122

3.454 ± 0.21

5.177 ± 0.217

3.425 ± 0.15

6.327 ± 0.195

5.934 ± 0.207

5.964 ± 0.22

7.39 ± 0.21

2.024 ± 0.121

2.86 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski