Paraprevotella clara CAG:116

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Paraprevotella; environmental samples

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5NCP3|R5NCP3_9BACT Tetratricopeptide repeat protein OS=Paraprevotella clara CAG:116 OX=1263095 GN=BN471_00637 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84KK3 pKa = 9.7VMFKK7 pKa = 9.77WLVMFLAVVLCAGVVSCKK25 pKa = 10.66DD26 pKa = 4.65DD27 pKa = 5.69DD28 pKa = 5.85DD29 pKa = 6.34DD30 pKa = 6.88DD31 pKa = 5.47NGASGNSIVGTWRR44 pKa = 11.84YY45 pKa = 10.84DD46 pKa = 3.42FGYY49 pKa = 10.53NAYY52 pKa = 10.74ALMTFNEE59 pKa = 4.89DD60 pKa = 2.49LTGIYY65 pKa = 9.7RR66 pKa = 11.84EE67 pKa = 3.99YY68 pKa = 11.16DD69 pKa = 3.54EE70 pKa = 6.06EE71 pKa = 6.19DD72 pKa = 3.77GGWWSYY78 pKa = 8.96TFTWLQNNGKK88 pKa = 10.09VMVIDD93 pKa = 5.35DD94 pKa = 3.75EE95 pKa = 6.03DD96 pKa = 6.03GYY98 pKa = 11.74GDD100 pKa = 3.51EE101 pKa = 4.82YY102 pKa = 11.82YY103 pKa = 11.02NVTVTATKK111 pKa = 8.93LTWTYY116 pKa = 10.36NNGYY120 pKa = 10.14GSEE123 pKa = 4.1KK124 pKa = 10.44QVWTRR129 pKa = 11.84QQQ131 pKa = 2.8

Molecular weight: 15.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5P143|R5P143_9BACT SusD family protein OS=Paraprevotella clara CAG:116 OX=1263095 GN=BN471_01740 PE=3 SV=1
MM1 pKa = 7.91PSGKK5 pKa = 9.66KK6 pKa = 9.73KK7 pKa = 9.79KK8 pKa = 7.27RR9 pKa = 11.84HH10 pKa = 5.32KK11 pKa = 10.0MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2847

0

2847

1089012

29

3967

382.5

43.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.522 ± 0.043

1.397 ± 0.017

5.82 ± 0.035

6.594 ± 0.044

4.496 ± 0.032

7.369 ± 0.046

2.023 ± 0.023

5.807 ± 0.041

5.82 ± 0.041

8.905 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.023

4.521 ± 0.041

3.881 ± 0.024

3.328 ± 0.025

5.285 ± 0.044

5.848 ± 0.033

5.695 ± 0.039

6.967 ± 0.037

1.398 ± 0.019

4.496 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski