Selenomonas ruminantium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes;

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2579 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1K1PXL5|A0A1K1PXL5_SELRU 30S ribosomal protein S6 OS=Selenomonas ruminantium OX=971 GN=rpsF PE=3 SV=1
MM1 pKa = 7.01GTIALIGIGVFLGWLMFRR19 pKa = 11.84PKK21 pKa = 10.53NDD23 pKa = 3.19NRR25 pKa = 11.84RR26 pKa = 11.84MSSADD31 pKa = 3.63LPQKK35 pKa = 10.21QQLTKK40 pKa = 10.35QQQTKK45 pKa = 7.86QQVTTGQRR53 pKa = 11.84PQVSSSLQHH62 pKa = 6.71RR63 pKa = 11.84EE64 pKa = 4.05NNSGNHH70 pKa = 4.76TLRR73 pKa = 11.84NVAGGMVAGAVLGHH87 pKa = 6.25MLSGDD92 pKa = 3.82HH93 pKa = 6.67KK94 pKa = 10.94AEE96 pKa = 3.78AHH98 pKa = 5.0EE99 pKa = 4.36TVNNYY104 pKa = 8.73NVYY107 pKa = 10.63NDD109 pKa = 4.28YY110 pKa = 11.17YY111 pKa = 10.91DD112 pKa = 4.57HH113 pKa = 6.96EE114 pKa = 5.35HH115 pKa = 6.84LAQDD119 pKa = 4.72EE120 pKa = 4.24DD121 pKa = 4.32VDD123 pKa = 3.83WDD125 pKa = 4.16DD126 pKa = 5.54DD127 pKa = 4.7YY128 pKa = 12.33DD129 pKa = 3.73MLAYY133 pKa = 10.46DD134 pKa = 4.82EE135 pKa = 6.22DD136 pKa = 6.48DD137 pKa = 4.3DD138 pKa = 6.28DD139 pKa = 5.97SYY141 pKa = 12.31NSYY144 pKa = 11.58DD145 pKa = 3.79DD146 pKa = 5.53DD147 pKa = 6.75DD148 pKa = 4.14YY149 pKa = 11.91TGYY152 pKa = 11.1EE153 pKa = 4.12DD154 pKa = 5.69DD155 pKa = 5.5SYY157 pKa = 11.93DD158 pKa = 4.3SSSDD162 pKa = 3.35WGSDD166 pKa = 3.28SYY168 pKa = 12.11DD169 pKa = 5.09DD170 pKa = 4.83GGDD173 pKa = 3.09WW174 pKa = 4.09

Molecular weight:
19.75 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1K1NKK7|A0A1K1NKK7_SELRU Uncharacterized protein OS=Selenomonas ruminantium OX=971 GN=SAMN02910323_1388 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 8.67MTFQPNNHH10 pKa = 5.38WRR12 pKa = 11.84KK13 pKa = 7.47KK14 pKa = 6.28THH16 pKa = 5.76GFRR19 pKa = 11.84EE20 pKa = 3.92RR21 pKa = 11.84MKK23 pKa = 10.1TKK25 pKa = 10.35GGRR28 pKa = 11.84LVLKK32 pKa = 10.33RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.62LSAA44 pKa = 4.03

Molecular weight:
5.44 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2579

0

2579

824202

37

2894

319.6

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.31 ± 0.053

1.259 ± 0.018

5.552 ± 0.044

7.007 ± 0.053

3.929 ± 0.031

7.452 ± 0.052

2.007 ± 0.024

6.425 ± 0.045

6.252 ± 0.042

9.487 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.224 ± 0.026

3.891 ± 0.04

3.682 ± 0.026

3.748 ± 0.037

4.744 ± 0.046

5.203 ± 0.035

5.136 ± 0.043

7.173 ± 0.045

1.044 ± 0.021

3.475 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski