Ralstonia phage phiAp1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; unclassified Krylovirinae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7DS50|A0A1L7DS50_9CAUD DUF5664 domain-containing protein OS=Ralstonia phage phiAp1 OX=2783867 GN=phiAp1_27 PE=4 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84DD3 pKa = 3.23VTDD6 pKa = 3.9IAAEE10 pKa = 3.81ALEE13 pKa = 4.77IIEE16 pKa = 4.63DD17 pKa = 3.8LKK19 pKa = 11.07KK20 pKa = 10.97AGVAGVLAGGCARR33 pKa = 11.84DD34 pKa = 3.36IAHH37 pKa = 7.54AIEE40 pKa = 4.66PKK42 pKa = 10.49DD43 pKa = 3.64YY44 pKa = 11.17DD45 pKa = 3.3IVVYY49 pKa = 9.75EE50 pKa = 4.25NQSSTVLIEE59 pKa = 4.32KK60 pKa = 10.12LMKK63 pKa = 10.4AGFYY67 pKa = 10.78ALEE70 pKa = 4.84PYY72 pKa = 11.05GDD74 pKa = 3.72TCSTRR79 pKa = 11.84EE80 pKa = 4.68DD81 pKa = 3.17IRR83 pKa = 11.84NDD85 pKa = 3.72LDD87 pKa = 4.61CVIHH91 pKa = 6.49GCAPNGAVCDD101 pKa = 3.92ILIYY105 pKa = 10.51TCDD108 pKa = 3.63FDD110 pKa = 5.16SPEE113 pKa = 4.02EE114 pKa = 3.99VVRR117 pKa = 11.84TFDD120 pKa = 3.84CTLNMAWLGVGEE132 pKa = 4.58FGQLSVNTVPDD143 pKa = 4.05YY144 pKa = 7.57PTPIQNFAEE153 pKa = 4.34VVPNVFVAGVDD164 pKa = 3.58DD165 pKa = 3.66TRR167 pKa = 11.84ARR169 pKa = 11.84YY170 pKa = 8.79IAGKK174 pKa = 9.36FPQYY178 pKa = 9.88IHH180 pKa = 6.55NRR182 pKa = 11.84TT183 pKa = 3.41

Molecular weight: 20.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L7DS43|A0A1L7DS43_9CAUD Putative DNA binding transcriptional regulator MarR family OS=Ralstonia phage phiAp1 OX=2783867 GN=phiAp1_20 PE=4 SV=1
MM1 pKa = 7.4SLVQSIYY8 pKa = 10.16FSKK11 pKa = 10.85LPAHH15 pKa = 6.67KK16 pKa = 10.19QPQRR20 pKa = 11.84KK21 pKa = 8.63PEE23 pKa = 4.08AGAGNRR29 pKa = 11.84ALRR32 pKa = 11.84GSDD35 pKa = 3.05KK36 pKa = 11.5YY37 pKa = 11.18NGPKK41 pKa = 10.13YY42 pKa = 10.35AVSKK46 pKa = 9.99DD47 pKa = 3.16TTTLGRR53 pKa = 11.84KK54 pKa = 7.61MLNALQKK61 pKa = 10.42ARR63 pKa = 11.84NSGSIVRR70 pKa = 11.84LLGDD74 pKa = 3.41TWHH77 pKa = 7.39INGTPMAII85 pKa = 3.65

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13833

35

1273

247.0

27.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.921 ± 0.62

1.012 ± 0.149

5.841 ± 0.211

5.451 ± 0.364

3.289 ± 0.186

7.699 ± 0.304

2.075 ± 0.224

4.446 ± 0.208

4.872 ± 0.302

8.068 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.214

4.048 ± 0.299

4.496 ± 0.194

4.692 ± 0.272

5.942 ± 0.323

5.754 ± 0.287

6.304 ± 0.376

6.795 ± 0.266

1.446 ± 0.146

2.993 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski