butyrate-producing bacterium SM4/1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4MRA2|D4MRA2_9FIRM Ankyrin repeat OS=butyrate-producing bacterium SM4/1 OX=245012 GN=CL3_17970 PE=4 SV=1
MM1 pKa = 7.81DD2 pKa = 5.72LEE4 pKa = 4.13NQKK7 pKa = 10.06RR8 pKa = 11.84VKK10 pKa = 10.63DD11 pKa = 3.74FAEE14 pKa = 4.07QYY16 pKa = 9.92GPEE19 pKa = 4.08NVVVILGAAEE29 pKa = 4.2GEE31 pKa = 4.4AAGLAAEE38 pKa = 4.67TVTAGDD44 pKa = 3.74PTFAGPLAGVQLGLTVYY61 pKa = 9.38HH62 pKa = 6.54ICEE65 pKa = 4.3DD66 pKa = 4.05EE67 pKa = 4.32IKK69 pKa = 11.06AEE71 pKa = 4.05VDD73 pKa = 2.98PGVYY77 pKa = 9.74DD78 pKa = 3.96DD79 pKa = 4.94QISMMEE85 pKa = 3.96MVMDD89 pKa = 3.7VDD91 pKa = 5.55DD92 pKa = 4.17IHH94 pKa = 8.55NEE96 pKa = 3.65MSSIRR101 pKa = 11.84DD102 pKa = 3.4QYY104 pKa = 11.45CKK106 pKa = 10.89YY107 pKa = 10.78LL108 pKa = 3.76

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4MRF6|D4MRF6_9FIRM Uncharacterized protein OS=butyrate-producing bacterium SM4/1 OX=245012 GN=CL3_18970 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.44VHH16 pKa = 5.57GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1684

0

1684

354993

13

2137

210.8

23.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.252 ± 0.073

1.634 ± 0.025

5.18 ± 0.052

7.976 ± 0.086

4.162 ± 0.05

7.793 ± 0.077

1.622 ± 0.028

6.522 ± 0.057

6.026 ± 0.058

9.274 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.142 ± 0.036

3.566 ± 0.041

3.665 ± 0.04

3.269 ± 0.04

5.751 ± 0.067

5.981 ± 0.056

5.013 ± 0.052

6.549 ± 0.067

0.999 ± 0.024

3.624 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski