Clostridium sp. CAG:678

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5KL51|R5KL51_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:678 OX=1262831 GN=BN753_01148 PE=4 SV=1
MM1 pKa = 7.96DD2 pKa = 5.11IDD4 pKa = 4.22SYY6 pKa = 11.91VYY8 pKa = 10.54DD9 pKa = 5.17AIFSEE14 pKa = 5.15TPKK17 pKa = 9.53HH18 pKa = 4.5TVFYY22 pKa = 10.82SLLWEE27 pKa = 4.08QFKK30 pKa = 11.21EE31 pKa = 4.13DD32 pKa = 3.72GFVITDD38 pKa = 3.72EE39 pKa = 4.79PGHH42 pKa = 6.39EE43 pKa = 4.0IDD45 pKa = 5.63CIFKK49 pKa = 10.5AVSMQNLMGEE59 pKa = 4.43FMYY62 pKa = 10.78RR63 pKa = 11.84LYY65 pKa = 11.29DD66 pKa = 3.66EE67 pKa = 4.9VNEE70 pKa = 4.45TGFEE74 pKa = 4.1DD75 pKa = 3.71AVDD78 pKa = 4.18YY79 pKa = 11.0IEE81 pKa = 5.03KK82 pKa = 10.76AGFTEE87 pKa = 5.55DD88 pKa = 6.29DD89 pKa = 3.2ILTYY93 pKa = 10.31CKK95 pKa = 9.85KK96 pKa = 10.68DD97 pKa = 3.35EE98 pKa = 4.89EE99 pKa = 4.43IEE101 pKa = 3.96IDD103 pKa = 3.97ASDD106 pKa = 3.57MDD108 pKa = 3.82ITAKK112 pKa = 10.56NALDD116 pKa = 3.45RR117 pKa = 11.84VTEE120 pKa = 4.15LTAEE124 pKa = 4.18KK125 pKa = 9.84MLEE128 pKa = 4.23EE129 pKa = 4.51YY130 pKa = 10.96SPDD133 pKa = 4.46DD134 pKa = 3.47IFDD137 pKa = 3.66MMFTAAYY144 pKa = 10.18DD145 pKa = 3.7FEE147 pKa = 4.86QDD149 pKa = 3.26FTFDD153 pKa = 4.91FEE155 pKa = 6.18DD156 pKa = 3.64SDD158 pKa = 3.83EE159 pKa = 4.34FLAFVDD165 pKa = 4.62ANTEE169 pKa = 3.75KK170 pKa = 10.73LDD172 pKa = 3.71QYY174 pKa = 11.02KK175 pKa = 10.44EE176 pKa = 4.09EE177 pKa = 4.32YY178 pKa = 9.51PGVINWIEE186 pKa = 3.75NGMICC191 pKa = 5.3

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5L2Q1|R5L2Q1_9CLOT Probable tRNA sulfurtransferase OS=Clostridium sp. CAG:678 OX=1262831 GN=thiI PE=3 SV=1
MM1 pKa = 7.65AKK3 pKa = 10.12CEE5 pKa = 4.08YY6 pKa = 9.51CGKK9 pKa = 10.66EE10 pKa = 3.42MTFGIKK16 pKa = 9.73VSHH19 pKa = 5.15SHH21 pKa = 6.16RR22 pKa = 11.84RR23 pKa = 11.84SNRR26 pKa = 11.84TWKK29 pKa = 10.42PNVKK33 pKa = 9.47RR34 pKa = 11.84VKK36 pKa = 10.47AIVNGSPKK44 pKa = 9.89RR45 pKa = 11.84VYY47 pKa = 10.56VCTRR51 pKa = 11.84CLRR54 pKa = 11.84SGKK57 pKa = 7.97VEE59 pKa = 3.97RR60 pKa = 11.84AA61 pKa = 3.22

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2250

0

2250

703918

29

2285

312.9

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.998 ± 0.056

1.718 ± 0.023

5.997 ± 0.047

6.825 ± 0.05

4.511 ± 0.041

6.839 ± 0.044

1.623 ± 0.023

7.343 ± 0.05

7.143 ± 0.051

8.432 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.023

5.063 ± 0.043

3.31 ± 0.028

2.952 ± 0.028

4.097 ± 0.036

6.316 ± 0.045

5.492 ± 0.046

6.707 ± 0.046

0.839 ± 0.019

4.25 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski