Avon-Heathcote Estuary associated circular virus 12

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 5.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMF1|A0A0C5IMF1_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 12 OX=1618235 PE=4 SV=1
MM1 pKa = 7.18QPLGLTMSGKK11 pKa = 10.28AKK13 pKa = 8.8QTINEE18 pKa = 3.96FDD20 pKa = 3.13IKK22 pKa = 11.23GNNTLVGVPQMAVMDD37 pKa = 4.14TAYY40 pKa = 10.76CLSKK44 pKa = 10.73LNHH47 pKa = 6.06RR48 pKa = 11.84LYY50 pKa = 10.69RR51 pKa = 11.84QGRR54 pKa = 11.84AYY56 pKa = 9.04TVRR59 pKa = 11.84VEE61 pKa = 4.2ANQDD65 pKa = 3.36SFGSTEE71 pKa = 4.21SIEE74 pKa = 4.62VFALMPTWYY83 pKa = 10.1LRR85 pKa = 11.84SAWRR89 pKa = 11.84MPKK92 pKa = 9.89KK93 pKa = 10.63AYY95 pKa = 10.24DD96 pKa = 3.43EE97 pKa = 4.81AMADD101 pKa = 3.88EE102 pKa = 4.83LASLNPEE109 pKa = 3.82NVARR113 pKa = 11.84WRR115 pKa = 11.84DD116 pKa = 3.52FRR118 pKa = 11.84VGSGITLTGTTDD130 pKa = 3.13ALEE133 pKa = 4.43FGGAVPNLWQLDD145 pKa = 3.99EE146 pKa = 4.47TGVAPVRR153 pKa = 11.84TPFTAGEE160 pKa = 4.22YY161 pKa = 9.39NLSYY165 pKa = 11.09AHH167 pKa = 6.84NLDD170 pKa = 3.68TGVNMQWWLKK180 pKa = 10.97GGDD183 pKa = 3.41ATHH186 pKa = 6.69FGVFEE191 pKa = 4.49EE192 pKa = 4.59YY193 pKa = 10.41SKK195 pKa = 11.33SRR197 pKa = 11.84NEE199 pKa = 4.07SASPEE204 pKa = 4.13TVIQSMPYY212 pKa = 9.33EE213 pKa = 4.09ALEE216 pKa = 4.56AEE218 pKa = 5.11GEE220 pKa = 4.19DD221 pKa = 3.49EE222 pKa = 5.97DD223 pKa = 5.15YY224 pKa = 11.78EE225 pKa = 4.33EE226 pKa = 4.34VQANGNEE233 pKa = 3.99PPYY236 pKa = 10.37NANAFPNGIWVRR248 pKa = 11.84VGVLEE253 pKa = 4.56KK254 pKa = 10.31STSLTQRR261 pKa = 11.84STGFFHH267 pKa = 7.54APLGMVAFF275 pKa = 5.25

Molecular weight: 30.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMF1|A0A0C5IMF1_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 12 OX=1618235 PE=4 SV=1
MM1 pKa = 6.86SQRR4 pKa = 11.84TRR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 10.08CLTLNNPTDD17 pKa = 3.69AEE19 pKa = 4.28IASLLDD25 pKa = 3.6VQEE28 pKa = 4.52GTLKK32 pKa = 10.64RR33 pKa = 11.84GFIALEE39 pKa = 4.1VGAEE43 pKa = 4.26GTPHH47 pKa = 6.29LQGFVHH53 pKa = 6.96LKK55 pKa = 8.71NAKK58 pKa = 9.21TGTALKK64 pKa = 10.64KK65 pKa = 10.15MLGSDD70 pKa = 2.95RR71 pKa = 11.84WHH73 pKa = 6.36WGKK76 pKa = 11.0ANGTDD81 pKa = 3.96FEE83 pKa = 4.22NWAYY87 pKa = 10.07IMEE90 pKa = 4.52DD91 pKa = 3.25VEE93 pKa = 5.57GKK95 pKa = 9.9VQGDD99 pKa = 4.48LLIQWGDD106 pKa = 3.24KK107 pKa = 9.61PAEE110 pKa = 4.29EE111 pKa = 5.08GEE113 pKa = 3.94PDD115 pKa = 3.19AWDD118 pKa = 4.45SILMMIEE125 pKa = 3.84AGEE128 pKa = 4.2DD129 pKa = 2.97DD130 pKa = 3.69RR131 pKa = 11.84AIVRR135 pKa = 11.84KK136 pKa = 8.39WPSIAIRR143 pKa = 11.84CQSAIAKK150 pKa = 9.36YY151 pKa = 9.65RR152 pKa = 11.84ASFEE156 pKa = 4.16WAEE159 pKa = 3.89CRR161 pKa = 11.84AWRR164 pKa = 11.84EE165 pKa = 3.79VEE167 pKa = 3.89VEE169 pKa = 4.38YY170 pKa = 10.75ISGPTGCGKK179 pKa = 8.2TRR181 pKa = 11.84EE182 pKa = 4.19ALYY185 pKa = 10.97LPDD188 pKa = 3.69GRR190 pKa = 11.84VNTDD194 pKa = 2.31VYY196 pKa = 11.0RR197 pKa = 11.84CTNGKK202 pKa = 9.16HH203 pKa = 5.88PFDD206 pKa = 4.18MYY208 pKa = 10.91DD209 pKa = 3.42GEE211 pKa = 4.42GTIVFEE217 pKa = 4.61EE218 pKa = 4.53FRR220 pKa = 11.84SQYY223 pKa = 7.73TCRR226 pKa = 11.84DD227 pKa = 3.19MLNWVDD233 pKa = 3.92GHH235 pKa = 6.69PLMLPARR242 pKa = 11.84YY243 pKa = 9.24ADD245 pKa = 3.61RR246 pKa = 11.84MAKK249 pKa = 7.73FTKK252 pKa = 10.51VIILSNWRR260 pKa = 11.84FEE262 pKa = 4.15EE263 pKa = 4.4QYY265 pKa = 9.54RR266 pKa = 11.84TVQADD271 pKa = 3.33SPEE274 pKa = 4.44TYY276 pKa = 10.03KK277 pKa = 10.95AWLRR281 pKa = 11.84RR282 pKa = 11.84VSTISEE288 pKa = 3.8RR289 pKa = 11.84WDD291 pKa = 3.1

Molecular weight: 33.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

566

275

291

283.0

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.834 ± 0.391

1.237 ± 0.55

5.654 ± 0.813

8.481 ± 0.074

3.71 ± 0.412

7.951 ± 0.26

1.59 ± 0.085

3.71 ± 0.964

4.24 ± 0.61

7.244 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.357 ± 0.176

4.594 ± 1.001

4.417 ± 0.425

3.357 ± 0.176

6.36 ± 1.03

5.3 ± 0.556

6.714 ± 0.352

6.007 ± 0.569

3.357 ± 0.512

3.887 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski