Streptomyces phage HotFries

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Scapunavirus; unclassified Scapunavirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UN55|A0A2U8UN55_9CAUD Terminase large subunit OS=Streptomyces phage HotFries OX=2182396 GN=6 PE=4 SV=1
MM1 pKa = 7.59SVLKK5 pKa = 10.0WDD7 pKa = 3.49QTGEE11 pKa = 3.98KK12 pKa = 9.88VYY14 pKa = 11.03EE15 pKa = 4.09NGVEE19 pKa = 3.85KK20 pKa = 10.91GVFYY24 pKa = 10.27TVNGAGAYY32 pKa = 10.26DD33 pKa = 5.21NGYY36 pKa = 9.53AWNGLVSVTEE46 pKa = 4.32SPSGAEE52 pKa = 3.98VNKK55 pKa = 10.3QYY57 pKa = 11.56ADD59 pKa = 3.34NRR61 pKa = 11.84VYY63 pKa = 11.23ASLRR67 pKa = 11.84SAEE70 pKa = 4.15EE71 pKa = 3.59FGATVEE77 pKa = 4.06AFTYY81 pKa = 9.27PKK83 pKa = 10.18QAIPALDD90 pKa = 3.84GAATPTPGLALGQQGRR106 pKa = 11.84ATFGLSYY113 pKa = 10.38VSKK116 pKa = 10.81VGNDD120 pKa = 3.62LNPDD124 pKa = 3.49AGEE127 pKa = 4.71KK128 pKa = 9.53IHH130 pKa = 6.59LVYY133 pKa = 10.5GATANPSEE141 pKa = 4.31KK142 pKa = 10.49AYY144 pKa = 7.99TTVNDD149 pKa = 3.83SPEE152 pKa = 3.78AATFSWEE159 pKa = 4.03LATTPVDD166 pKa = 3.21VGTIAGTDD174 pKa = 3.66YY175 pKa = 11.18KK176 pKa = 10.3PLSTITIDD184 pKa = 3.57TTQEE188 pKa = 3.91DD189 pKa = 4.36PDD191 pKa = 3.75AVATLRR197 pKa = 11.84EE198 pKa = 4.39FLYY201 pKa = 9.05GTAGTDD207 pKa = 3.36PSLPSPAAVVALFSGAVLTATPTEE231 pKa = 4.1PTYY234 pKa = 10.77DD235 pKa = 3.04TTTNVMTIPSITGVEE250 pKa = 4.47YY251 pKa = 11.52YY252 pKa = 9.96MDD254 pKa = 4.71DD255 pKa = 3.43QLLAAGPQPALTTNKK270 pKa = 9.39VVEE273 pKa = 4.17ARR275 pKa = 11.84PAPGYY280 pKa = 10.94KK281 pKa = 8.85FTQPVDD287 pKa = 3.71NDD289 pKa = 3.51WLIGDD294 pKa = 4.49FF295 pKa = 4.65

Molecular weight: 31.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UP62|A0A2U8UP62_9CAUD Uncharacterized protein OS=Streptomyces phage HotFries OX=2182396 GN=38 PE=4 SV=1
MM1 pKa = 7.65GFRR4 pKa = 11.84FGKK7 pKa = 10.06NSRR10 pKa = 11.84GEE12 pKa = 4.04NSVKK16 pKa = 9.82RR17 pKa = 11.84QRR19 pKa = 11.84WSISPYY25 pKa = 9.45EE26 pKa = 4.24IKK28 pKa = 10.85NGAKK32 pKa = 9.93QFGLAIWWNSNRR44 pKa = 11.84VGFQINIWVWEE55 pKa = 4.05IQVMRR60 pKa = 11.84EE61 pKa = 3.69NN62 pKa = 3.67

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13632

41

1742

239.2

26.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.69 ± 0.616

0.396 ± 0.087

6.411 ± 0.339

6.433 ± 0.449

3.712 ± 0.253

7.592 ± 0.488

1.599 ± 0.201

5.428 ± 0.264

6.294 ± 0.507

8.091 ± 0.531

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.107

3.983 ± 0.146

4.203 ± 0.302

3.316 ± 0.154

5.054 ± 0.457

5.817 ± 0.329

7.057 ± 0.46

7.372 ± 0.259

1.585 ± 0.192

3.433 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski