Rubidibacter lacunae KORDI 51-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Rubidibacter; Rubidibacter lacunae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5DC01|U5DC01_9CHRO Malate dehydrogenase OS=Rubidibacter lacunae KORDI 51-2 OX=582515 GN=mdh PE=3 SV=1
MM1 pKa = 7.45IANHH5 pKa = 5.74VKK7 pKa = 10.62ALLHH11 pKa = 6.97KK12 pKa = 10.53NGCHH16 pKa = 6.11WNTGGSVGNSSGFGGSAAITYY37 pKa = 10.47SFMDD41 pKa = 3.72GRR43 pKa = 11.84PAGFDD48 pKa = 3.22PDD50 pKa = 3.7QFQDD54 pKa = 3.59FADD57 pKa = 5.93FSATQASWIEE67 pKa = 3.99HH68 pKa = 6.28AMDD71 pKa = 3.97HH72 pKa = 6.15YY73 pKa = 11.61EE74 pKa = 4.41EE75 pKa = 4.47IANITFAEE83 pKa = 4.56VASGGDD89 pKa = 3.33IDD91 pKa = 3.79WGYY94 pKa = 11.33AQTFYY99 pKa = 11.31DD100 pKa = 3.51WDD102 pKa = 3.86GDD104 pKa = 4.16GVIEE108 pKa = 4.15SHH110 pKa = 6.02EE111 pKa = 4.32VAGGLATRR119 pKa = 11.84PYY121 pKa = 10.16SNGSIIVIDD130 pKa = 3.67RR131 pKa = 11.84DD132 pKa = 3.72STNFSQGSWGYY143 pKa = 10.17LVLMHH148 pKa = 6.61EE149 pKa = 5.26LGHH152 pKa = 6.67AAAGFNDD159 pKa = 3.55VTIGLNFSEE168 pKa = 5.46TYY170 pKa = 7.19TTSQEE175 pKa = 3.89YY176 pKa = 9.27WNQGIDD182 pKa = 3.77GQVLSVSEE190 pKa = 4.52DD191 pKa = 3.26SRR193 pKa = 11.84KK194 pKa = 7.6YY195 pKa = 9.27TAMSYY200 pKa = 11.03NNHH203 pKa = 6.66PDD205 pKa = 3.12MPGVHH210 pKa = 7.16PNTLLLYY217 pKa = 10.45DD218 pKa = 4.02IAALQTLYY226 pKa = 11.07GANTSTRR233 pKa = 11.84AGNTTYY239 pKa = 10.73SWATNEE245 pKa = 4.37TFLEE249 pKa = 4.77TIWDD253 pKa = 3.82GGGTDD258 pKa = 4.51TISASNQTRR267 pKa = 11.84DD268 pKa = 3.52AVINLNPGTFSSIGSYY284 pKa = 10.31AGRR287 pKa = 11.84DD288 pKa = 3.48ATDD291 pKa = 3.23NLAIAFNVTIEE302 pKa = 3.96NAYY305 pKa = 10.04GGSGNDD311 pKa = 2.67RR312 pKa = 11.84VFGNAVGNEE321 pKa = 3.66IRR323 pKa = 11.84GGAGNDD329 pKa = 2.99RR330 pKa = 11.84LFGQDD335 pKa = 2.88GNDD338 pKa = 3.08RR339 pKa = 11.84LFGEE343 pKa = 5.12DD344 pKa = 3.3GNDD347 pKa = 3.06RR348 pKa = 11.84LFGQDD353 pKa = 4.2GNDD356 pKa = 3.4FLYY359 pKa = 10.72GQDD362 pKa = 4.25GNDD365 pKa = 3.41FLYY368 pKa = 10.73GQDD371 pKa = 4.31GDD373 pKa = 4.2DD374 pKa = 3.84FLYY377 pKa = 10.78GHH379 pKa = 7.95DD380 pKa = 3.97DD381 pKa = 3.4TDD383 pKa = 3.84RR384 pKa = 11.84LSGEE388 pKa = 4.44DD389 pKa = 3.86GNDD392 pKa = 2.87RR393 pKa = 11.84LYY395 pKa = 11.14GQEE398 pKa = 4.55GNDD401 pKa = 3.51FLYY404 pKa = 10.88GGNGDD409 pKa = 4.07DD410 pKa = 4.57FLYY413 pKa = 10.56GQEE416 pKa = 4.73GNDD419 pKa = 3.51FLYY422 pKa = 10.88GGNGDD427 pKa = 4.27DD428 pKa = 3.89RR429 pKa = 11.84QSGGKK434 pKa = 10.21GDD436 pKa = 3.75DD437 pKa = 3.13RR438 pKa = 11.84LYY440 pKa = 11.43GEE442 pKa = 4.83NGNDD446 pKa = 3.37FLYY449 pKa = 11.17GEE451 pKa = 4.76NGNDD455 pKa = 3.11SLYY458 pKa = 11.04GGKK461 pKa = 10.39GDD463 pKa = 4.33DD464 pKa = 3.48RR465 pKa = 11.84LYY467 pKa = 11.41GKK469 pKa = 10.07DD470 pKa = 4.14GNDD473 pKa = 4.45SLYY476 pKa = 10.81GWQGNDD482 pKa = 2.75RR483 pKa = 11.84LYY485 pKa = 11.43GEE487 pKa = 4.97NGNDD491 pKa = 3.19SLYY494 pKa = 11.31GEE496 pKa = 4.88NGNDD500 pKa = 3.11SLYY503 pKa = 10.85GGKK506 pKa = 10.62GSDD509 pKa = 3.62VLIGGAGADD518 pKa = 3.54TLVGFGSSSSQFDD531 pKa = 3.92TLTGGAGTDD540 pKa = 3.51TFVLGDD546 pKa = 4.29LGSVFYY552 pKa = 10.74LGSGHH557 pKa = 7.22ARR559 pKa = 11.84ITDD562 pKa = 4.07FTSGSLAIEE571 pKa = 3.94DD572 pKa = 4.42TIQIRR577 pKa = 11.84GVLNDD582 pKa = 3.29YY583 pKa = 8.41TLNQSVNYY591 pKa = 9.7GGAAALDD598 pKa = 3.7TAIFLGSDD606 pKa = 4.23LIGVVEE612 pKa = 4.65DD613 pKa = 3.77TTAIALTADD622 pKa = 3.51YY623 pKa = 7.31FTTVV627 pKa = 2.69

Molecular weight: 66.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5DNG3|U5DNG3_9CHRO Uncharacterized protein OS=Rubidibacter lacunae KORDI 51-2 OX=582515 GN=KR51_00009370 PE=4 SV=1
MM1 pKa = 7.37AQQTLRR7 pKa = 11.84GTRR10 pKa = 11.84LKK12 pKa = 10.5QKK14 pKa = 10.2RR15 pKa = 11.84KK16 pKa = 9.06LGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 9.65TGRR29 pKa = 11.84RR30 pKa = 11.84VINARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.42GRR40 pKa = 11.84ARR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3455

0

3455

1058787

24

13395

306.5

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.756 ± 0.061

1.138 ± 0.022

5.787 ± 0.062

5.975 ± 0.04

3.763 ± 0.026

7.658 ± 0.055

1.834 ± 0.028

5.175 ± 0.032

2.737 ± 0.041

11.224 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.025

2.987 ± 0.034

5.315 ± 0.035

4.187 ± 0.039

7.079 ± 0.053

5.993 ± 0.033

5.419 ± 0.055

7.153 ± 0.04

1.488 ± 0.021

2.648 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski