Blastococcus endophyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4606 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8W336|A0A1H8W336_9ACTN DNA-binding transcriptional regulator MarR family OS=Blastococcus endophyticus OX=673521 GN=SAMN05660991_03983 PE=4 SV=1
MM1 pKa = 7.4TPLGSSGRR9 pKa = 11.84RR10 pKa = 11.84VRR12 pKa = 11.84ATTAGIAVTALALTACGGGDD32 pKa = 4.29DD33 pKa = 5.31DD34 pKa = 5.15NGSGGSGGSADD45 pKa = 3.79ALIVGTTDD53 pKa = 3.92KK54 pKa = 10.38ITTIDD59 pKa = 3.49PAGSYY64 pKa = 11.17DD65 pKa = 3.54NGSFAVMNQVYY76 pKa = 9.38PFLLNTPLGSPEE88 pKa = 4.13VEE90 pKa = 3.58PDD92 pKa = 2.97IAEE95 pKa = 4.42SADD98 pKa = 3.54FTSPTQYY105 pKa = 10.7TVTLKK110 pKa = 10.81PGLTFANGNEE120 pKa = 4.04LTASDD125 pKa = 4.03VKK127 pKa = 10.85FSFDD131 pKa = 3.4RR132 pKa = 11.84QVAIADD138 pKa = 3.83EE139 pKa = 4.69NGPSSLLANLEE150 pKa = 4.4SVEE153 pKa = 4.61APDD156 pKa = 3.59DD157 pKa = 3.75TTVVFNLTNPDD168 pKa = 3.74DD169 pKa = 3.76QTFPQVLSSPVGPIVDD185 pKa = 3.98EE186 pKa = 4.58EE187 pKa = 4.61VFSADD192 pKa = 3.86SLTPDD197 pKa = 3.9DD198 pKa = 5.53EE199 pKa = 4.54IVAGNAFAGPYY210 pKa = 10.1VITSYY215 pKa = 10.76EE216 pKa = 4.03INDD219 pKa = 4.67LIAYY223 pKa = 7.35EE224 pKa = 4.76ANPDD228 pKa = 3.66YY229 pKa = 10.91QGLLGAPKK237 pKa = 9.67TDD239 pKa = 4.01TINVRR244 pKa = 11.84YY245 pKa = 9.04YY246 pKa = 11.41AEE248 pKa = 4.26ASNLKK253 pKa = 10.33LDD255 pKa = 3.79IEE257 pKa = 4.46EE258 pKa = 4.47GAVDD262 pKa = 3.24VAYY265 pKa = 10.47RR266 pKa = 11.84SLSATDD272 pKa = 3.87VEE274 pKa = 4.61DD275 pKa = 4.59LRR277 pKa = 11.84GNDD280 pKa = 3.46DD281 pKa = 3.71VEE283 pKa = 4.74VVDD286 pKa = 5.11GPGGEE291 pKa = 3.55IRR293 pKa = 11.84YY294 pKa = 9.33IVFNFNTQPYY304 pKa = 9.14GATTAEE310 pKa = 4.31ADD312 pKa = 3.66PAKK315 pKa = 10.67ALAVRR320 pKa = 11.84QAMAHH325 pKa = 6.32LIDD328 pKa = 4.28RR329 pKa = 11.84EE330 pKa = 4.02EE331 pKa = 4.27LAEE334 pKa = 3.89QVYY337 pKa = 10.47KK338 pKa = 10.16GTYY341 pKa = 7.52TPLYY345 pKa = 10.12SYY347 pKa = 10.96VADD350 pKa = 4.27GLTGATDD357 pKa = 3.71SLRR360 pKa = 11.84GLYY363 pKa = 10.57GDD365 pKa = 4.08GQGGPDD371 pKa = 3.86ADD373 pKa = 3.79AAAQVLEE380 pKa = 4.23AAGVEE385 pKa = 4.58TPVQLSLQYY394 pKa = 11.43SNDD397 pKa = 3.17HH398 pKa = 6.35YY399 pKa = 11.31GPSSGDD405 pKa = 3.07EE406 pKa = 3.82YY407 pKa = 11.92ALIKK411 pKa = 10.68DD412 pKa = 3.77QLEE415 pKa = 4.36STGLFTVDD423 pKa = 3.92LQTTEE428 pKa = 3.39WTQYY432 pKa = 10.48SEE434 pKa = 4.06QRR436 pKa = 11.84TADD439 pKa = 3.6TYY441 pKa = 10.9PAYY444 pKa = 10.2QLGWFPDD451 pKa = 3.67YY452 pKa = 11.48SDD454 pKa = 4.27ADD456 pKa = 3.57NYY458 pKa = 10.67LAPFFLTEE466 pKa = 4.09NFLSNHH472 pKa = 6.16YY473 pKa = 10.76DD474 pKa = 3.48NQQVNDD480 pKa = 5.53LILQQLSTPDD490 pKa = 3.13EE491 pKa = 4.24AARR494 pKa = 11.84TALIEE499 pKa = 4.06QIQDD503 pKa = 3.38LVAADD508 pKa = 5.24LSTLPYY514 pKa = 10.43LQGAQIAVVRR524 pKa = 11.84TGVTGAEE531 pKa = 4.21DD532 pKa = 3.46TLDD535 pKa = 3.3ASFKK539 pKa = 10.23FRR541 pKa = 11.84YY542 pKa = 9.05GALEE546 pKa = 3.81RR547 pKa = 11.84AA548 pKa = 4.11

Molecular weight: 58.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8WJV0|A0A1H8WJV0_9ACTN Methyltransferase domain-containing protein OS=Blastococcus endophyticus OX=673521 GN=SAMN05660991_04484 PE=4 SV=1
MM1 pKa = 7.12LTVALAWTAVLASVVAAALFGQTARR26 pKa = 11.84GGAPRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SVARR39 pKa = 11.84TRR41 pKa = 11.84AGLAVAMSGPWLVLVLGVAAGAVTGAWLAAAAGTVAGVVAVATAGLVLVPRR92 pKa = 4.98

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4606

0

4606

1481554

39

1827

321.7

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.708 ± 0.057

0.705 ± 0.011

6.297 ± 0.029

5.64 ± 0.031

2.598 ± 0.021

9.836 ± 0.032

1.971 ± 0.016

2.818 ± 0.025

1.275 ± 0.022

10.592 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.636 ± 0.015

1.411 ± 0.016

6.35 ± 0.038

2.521 ± 0.02

8.198 ± 0.037

4.689 ± 0.025

5.831 ± 0.025

9.699 ± 0.04

1.462 ± 0.015

1.762 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski