Paenibacillus sp. BK673

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6N6N9|A0A4R6N6N9_9BACL Uracil-DNA glycosylase OS=Paenibacillus sp. BK673 OX=2512134 GN=ung PE=3 SV=1
MM1 pKa = 7.55VITEE5 pKa = 4.22SAILMLMSSLHH16 pKa = 6.38VDD18 pKa = 3.39VNVDD22 pKa = 2.22VDD24 pKa = 3.38MGVNVDD30 pKa = 3.13VDD32 pKa = 3.55VDD34 pKa = 3.68VDD36 pKa = 3.63VDD38 pKa = 3.63VDD40 pKa = 3.63VDD42 pKa = 3.63VDD44 pKa = 3.63VDD46 pKa = 3.63VDD48 pKa = 3.63VDD50 pKa = 3.63VDD52 pKa = 3.63VDD54 pKa = 3.6VDD56 pKa = 3.84VDD58 pKa = 3.87VGCCHH63 pKa = 6.84PRR65 pKa = 11.84LFIPSHH71 pKa = 4.27QRR73 pKa = 11.84YY74 pKa = 7.17EE75 pKa = 3.92FRR77 pKa = 11.84YY78 pKa = 9.27EE79 pKa = 3.95YY80 pKa = 10.48IDD82 pKa = 3.95IITIYY87 pKa = 10.41RR88 pKa = 11.84IQQ90 pKa = 3.54

Molecular weight: 10.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6MV69|A0A4R6MV69_9BACL Serine/threonine protein phosphatase PrpC OS=Paenibacillus sp. BK673 OX=2512134 GN=EV295_105252 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.37RR3 pKa = 11.84GILLIGGLVLMVLLCWLSIAAGAVPITAPQVLSGLTEE40 pKa = 4.18PQSPSYY46 pKa = 10.51FIVHH50 pKa = 6.54EE51 pKa = 4.05LRR53 pKa = 11.84LPRR56 pKa = 11.84VLVAICAGAGLAVAGAILQSLLRR79 pKa = 11.84NPLASPDD86 pKa = 3.88VIGITQGASFAAVALIFLLPGAAAQWMPVAAFAGAMLSFLILILTSRR133 pKa = 11.84RR134 pKa = 11.84LTLSPASLALVGVAVGAVFQAGIQYY159 pKa = 10.33FMVTHH164 pKa = 6.82PANVNMALLWMSGSLWGRR182 pKa = 11.84GWDD185 pKa = 3.44QLTPLAPLIVLMLTIVLLQARR206 pKa = 11.84KK207 pKa = 10.17LNILQIGDD215 pKa = 4.8DD216 pKa = 4.09LSQSLGLFVRR226 pKa = 11.84KK227 pKa = 9.03EE228 pKa = 4.15RR229 pKa = 11.84LWLMLLSVTLAGVAVSAVGAVSFIGLLPPHH259 pKa = 7.43IARR262 pKa = 11.84ALVGARR268 pKa = 11.84NQWRR272 pKa = 11.84LPVAAVIGADD282 pKa = 4.76LMLLGDD288 pKa = 3.7WVGRR292 pKa = 11.84TVLIPRR298 pKa = 11.84EE299 pKa = 4.07IPVGIVIAIIGAPYY313 pKa = 10.26FIYY316 pKa = 10.78LLRR319 pKa = 11.84RR320 pKa = 11.84EE321 pKa = 4.82HH322 pKa = 7.04KK323 pKa = 9.97MKK325 pKa = 10.85

Molecular weight: 34.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6028

0

6028

1882351

25

8979

312.3

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.133 ± 0.031

0.783 ± 0.01

5.125 ± 0.026

6.971 ± 0.039

3.771 ± 0.021

7.305 ± 0.031

2.284 ± 0.021

6.498 ± 0.03

4.8 ± 0.03

10.119 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.018

3.689 ± 0.026

4.014 ± 0.022

4.42 ± 0.029

5.226 ± 0.028

6.479 ± 0.029

5.586 ± 0.036

7.026 ± 0.026

1.269 ± 0.011

3.579 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski