Synechococcus sp. (strain CC9311)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2882 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q0I7U1|ATPB_SYNS3 ATP synthase subunit beta OS=Synechococcus sp. (strain CC9311) OX=64471 GN=atpD PE=3 SV=1
MM1 pKa = 7.57TMPPYY6 pKa = 10.45QIFQCDD12 pKa = 3.49VEE14 pKa = 4.44DD15 pKa = 3.61EE16 pKa = 4.63KK17 pKa = 11.13IYY19 pKa = 11.14GNYY22 pKa = 7.31EE23 pKa = 3.9TLEE26 pKa = 4.12LAKK29 pKa = 9.87MAWADD34 pKa = 3.58IIARR38 pKa = 11.84KK39 pKa = 10.29NEE41 pKa = 3.71MSVIAMEE48 pKa = 4.49LVDD51 pKa = 6.02DD52 pKa = 4.22EE53 pKa = 5.91LEE55 pKa = 4.22VLEE58 pKa = 4.5TFYY61 pKa = 10.95FYY63 pKa = 11.54

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0I8I4|Q0I8I4_SYNS3 Uncharacterized protein OS=Synechococcus sp. (strain CC9311) OX=64471 GN=sync_2035 PE=4 SV=1
MM1 pKa = 7.41TKK3 pKa = 9.05RR4 pKa = 11.84TFGGTSRR11 pKa = 11.84KK12 pKa = 9.13RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92RR38 pKa = 11.84GRR40 pKa = 11.84SRR42 pKa = 11.84LAAA45 pKa = 4.33

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2882

0

2882

752638

27

2178

261.2

28.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.597 ± 0.055

1.304 ± 0.017

5.281 ± 0.038

5.785 ± 0.048

3.344 ± 0.031

7.963 ± 0.045

2.09 ± 0.024

4.859 ± 0.045

3.208 ± 0.04

12.282 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.023

3.107 ± 0.038

5.24 ± 0.035

4.686 ± 0.04

6.678 ± 0.048

7.041 ± 0.042

4.885 ± 0.039

6.888 ± 0.047

1.701 ± 0.026

1.887 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski