Clostridium sp. CAG:230

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6DE79|R6DE79_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:230 OX=1262782 GN=BN547_01162 PE=4 SV=1
MM1 pKa = 7.52AGVSGVNGIDD11 pKa = 3.68EE12 pKa = 4.29EE13 pKa = 4.5TLYY16 pKa = 10.83YY17 pKa = 10.44QYY19 pKa = 11.29LINHH23 pKa = 6.37NSASTMLNALSGDD36 pKa = 3.85SSDD39 pKa = 4.58DD40 pKa = 3.75DD41 pKa = 4.82SMLSGISSLSGLSGLSGTSGLSGLSGVSQDD71 pKa = 3.86SSFATILQSYY81 pKa = 6.82LTKK84 pKa = 10.57AIHH87 pKa = 5.77SQNDD91 pKa = 3.49SAQDD95 pKa = 3.14AQMAEE100 pKa = 4.25KK101 pKa = 10.61LSDD104 pKa = 3.71VLQEE108 pKa = 3.9AAKK111 pKa = 10.13TEE113 pKa = 4.25DD114 pKa = 3.67TSNTTYY120 pKa = 9.43KK121 pKa = 9.94TVQEE125 pKa = 4.04LYY127 pKa = 10.56EE128 pKa = 4.18YY129 pKa = 9.21FTEE132 pKa = 4.54KK133 pKa = 10.24MSGTAASLINGSAAAQSTNSTNSTNSTQNSTSAQSTAEE171 pKa = 3.86QMNQAALVGQEE182 pKa = 4.16FDD184 pKa = 3.5FSKK187 pKa = 10.28IDD189 pKa = 3.85SIVEE193 pKa = 3.86QMFSEE198 pKa = 4.48STPLSS203 pKa = 3.58

Molecular weight: 21.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6EDC6|R6EDC6_9CLOT Beta-xylosidase (1 4-beta-D-xylan xylohydrolase) (Xylan1 4-beta-xylosidase) OS=Clostridium sp. CAG:230 OX=1262782 GN=BN547_00179 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.02KK10 pKa = 9.86SRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.65GFRR19 pKa = 11.84KK20 pKa = 10.1RR21 pKa = 11.84MQTSNGRR28 pKa = 11.84KK29 pKa = 8.52VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.27GRR39 pKa = 11.84HH40 pKa = 5.48KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2209

0

2209

717718

30

1528

324.9

36.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.274 ± 0.049

1.492 ± 0.022

5.807 ± 0.043

7.206 ± 0.064

4.045 ± 0.042

6.462 ± 0.047

1.838 ± 0.026

7.553 ± 0.051

8.148 ± 0.05

8.53 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.097 ± 0.028

4.509 ± 0.031

2.9 ± 0.029

3.654 ± 0.034

3.913 ± 0.041

5.995 ± 0.045

5.706 ± 0.052

6.804 ± 0.05

0.824 ± 0.018

4.239 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski