Escherichia phage Av-05

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Avunavirus; Escherichia virus Av05

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076G6W2|A0A076G6W2_9CAUD Uncharacterized protein OS=Escherichia phage Av-05 OX=1527519 GN=Av05_00202 PE=4 SV=1
MM1 pKa = 7.74AKK3 pKa = 10.39LIIEE7 pKa = 4.36GSEE10 pKa = 4.04DD11 pKa = 3.47VLKK14 pKa = 11.17CFAAWFSCSGEE25 pKa = 3.93QSFTEE30 pKa = 4.31AFRR33 pKa = 11.84MGDD36 pKa = 2.74ITGNYY41 pKa = 6.44PTTDD45 pKa = 3.17IIVRR49 pKa = 11.84GYY51 pKa = 10.59GINEE55 pKa = 4.97PIQLVEE61 pKa = 4.14YY62 pKa = 10.82DD63 pKa = 3.76LATDD67 pKa = 3.6EE68 pKa = 5.02EE69 pKa = 4.67IPYY72 pKa = 10.43VDD74 pKa = 3.51

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076G7X8|A0A076G7X8_9CAUD Uncharacterized protein OS=Escherichia phage Av-05 OX=1527519 GN=Av05_0054 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.29RR3 pKa = 11.84FTYY6 pKa = 10.04GVVGGSVSNRR16 pKa = 11.84FYY18 pKa = 10.14NTEE21 pKa = 3.86RR22 pKa = 11.84GAKK25 pKa = 8.6NAATRR30 pKa = 11.84EE31 pKa = 4.25HH32 pKa = 6.68GNSVNLQVGYY42 pKa = 10.09ISKK45 pKa = 10.06ISGMFIPVAQRR56 pKa = 11.84GMYY59 pKa = 9.88CIGWMPMM66 pKa = 3.21

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

209

0

209

35279

39

793

168.8

19.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.896 ± 0.253

1.332 ± 0.09

6.42 ± 0.156

6.673 ± 0.196

4.274 ± 0.109

6.607 ± 0.154

1.808 ± 0.089

6.457 ± 0.137

7.721 ± 0.243

8.149 ± 0.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.108

5.105 ± 0.168

3.617 ± 0.136

3.387 ± 0.113

4.345 ± 0.16

6.157 ± 0.186

5.712 ± 0.253

6.752 ± 0.203

1.667 ± 0.094

4.15 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski