Hubei myriapoda virus 8

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Jingchuvirales; Myriaviridae; Myriavirus; Myriavirus myriapedis

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN57|A0A1L3KN57_9VIRU Putative glycoprotein OS=Hubei myriapoda virus 8 OX=1922937 PE=4 SV=1
MM1 pKa = 7.59AGAPPFVGLVWQTVLDD17 pKa = 4.14CFKK20 pKa = 10.93DD21 pKa = 3.78LPDD24 pKa = 3.89EE25 pKa = 4.08QKK27 pKa = 10.59LWFGHH32 pKa = 5.6VYY34 pKa = 10.72GGGTGATDD42 pKa = 4.69LIARR46 pKa = 11.84EE47 pKa = 4.15SCLLAMICLKK57 pKa = 10.67YY58 pKa = 10.13RR59 pKa = 11.84VEE61 pKa = 4.1VLRR64 pKa = 11.84QEE66 pKa = 3.93LAIRR70 pKa = 11.84EE71 pKa = 4.14EE72 pKa = 4.33GGLAIKK78 pKa = 9.26TVEE81 pKa = 3.85VLMTEE86 pKa = 4.47NIDD89 pKa = 4.66DD90 pKa = 4.3AMCAPADD97 pKa = 3.96MALHH101 pKa = 7.46WITSALEE108 pKa = 4.3HH109 pKa = 6.27YY110 pKa = 10.34CRR112 pKa = 11.84TPFNVSGVWNNADD125 pKa = 2.86WFEE128 pKa = 4.06YY129 pKa = 10.79DD130 pKa = 3.83HH131 pKa = 7.63IIVAEE136 pKa = 4.28AGAVPAHH143 pKa = 6.29FGGLRR148 pKa = 11.84EE149 pKa = 3.97NHH151 pKa = 6.33GFPGVEE157 pKa = 4.01GNIVRR162 pKa = 11.84ARR164 pKa = 11.84LIRR167 pKa = 11.84DD168 pKa = 3.78LHH170 pKa = 6.69SKK172 pKa = 11.15ADD174 pKa = 3.54VKK176 pKa = 11.14AILVFLLRR184 pKa = 11.84EE185 pKa = 3.91AAVVDD190 pKa = 3.79RR191 pKa = 11.84AEE193 pKa = 4.23RR194 pKa = 11.84GMHH197 pKa = 6.54KK198 pKa = 10.44LFAHH202 pKa = 6.62FFCALSQRR210 pKa = 11.84GNATEE215 pKa = 3.46NWIAKK220 pKa = 8.71RR221 pKa = 11.84QEE223 pKa = 3.88DD224 pKa = 4.92FRR226 pKa = 11.84NQLNDD231 pKa = 3.01QTFVLNSLAMQIIWKK246 pKa = 8.89NWLSKK251 pKa = 10.52IVFPEE256 pKa = 3.97LKK258 pKa = 10.02FVNSLAYY265 pKa = 10.67LEE267 pKa = 5.11ANQLKK272 pKa = 9.04TGQRR276 pKa = 11.84MMLTVEE282 pKa = 4.12HH283 pKa = 6.36SAYY286 pKa = 9.78KK287 pKa = 10.68GLAAYY292 pKa = 9.37GFISSAFNQLPAEE305 pKa = 4.26SFAWAALDD313 pKa = 3.3EE314 pKa = 5.33CIPQNEE320 pKa = 3.74MDD322 pKa = 3.91AYY324 pKa = 10.16EE325 pKa = 4.06IARR328 pKa = 11.84DD329 pKa = 3.65IILEE333 pKa = 4.35GSHH336 pKa = 6.43AMYY339 pKa = 8.29GTRR342 pKa = 11.84EE343 pKa = 3.81VQNRR347 pKa = 11.84LKK349 pKa = 9.93ATRR352 pKa = 11.84YY353 pKa = 8.82PSLAYY358 pKa = 10.3FGVQYY363 pKa = 9.63ATRR366 pKa = 11.84VVRR369 pKa = 11.84NIMANGYY376 pKa = 9.79RR377 pKa = 11.84GVGSNEE383 pKa = 3.22KK384 pKa = 10.47DD385 pKa = 3.47VANRR389 pKa = 11.84ALIDD393 pKa = 4.19EE394 pKa = 5.3IITRR398 pKa = 11.84LQQEE402 pKa = 4.61LGRR405 pKa = 11.84RR406 pKa = 11.84DD407 pKa = 3.84YY408 pKa = 11.12IAGIEE413 pKa = 4.03QNAEE417 pKa = 3.75GRR419 pKa = 11.84NLRR422 pKa = 11.84YY423 pKa = 10.09RR424 pKa = 11.84MLALDD429 pKa = 4.59NPNDD433 pKa = 3.97GNDD436 pKa = 3.07VV437 pKa = 3.39

Molecular weight: 49.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KN84|A0A1L3KN84_9VIRU Uncharacterized protein OS=Hubei myriapoda virus 8 OX=1922937 PE=4 SV=1
MM1 pKa = 7.47TSFNATGLPTIRR13 pKa = 11.84PGRR16 pKa = 11.84SASVPPSTEE25 pKa = 3.8GAHH28 pKa = 7.2DD29 pKa = 3.55IQKK32 pKa = 10.04PAMFSTMPTAGTLLKK47 pKa = 8.4PTPPYY52 pKa = 9.04MHH54 pKa = 8.16PDD56 pKa = 3.33RR57 pKa = 11.84PLTKK61 pKa = 10.28GDD63 pKa = 3.54FDD65 pKa = 3.71IAMKK69 pKa = 10.6RR70 pKa = 11.84LEE72 pKa = 4.13AQIADD77 pKa = 3.74LTQNVHH83 pKa = 5.12QSIRR87 pKa = 11.84LHH89 pKa = 5.26QQAQGFGTSYY99 pKa = 11.23APPTAPSLYY108 pKa = 9.87PDD110 pKa = 3.46MTEE113 pKa = 4.14QLHH116 pKa = 5.89QGLDD120 pKa = 3.23RR121 pKa = 11.84YY122 pKa = 10.51LL123 pKa = 4.94

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3956

123

2379

989.0

113.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.612 ± 1.467

1.668 ± 0.128

5.207 ± 0.481

6.041 ± 0.57

4.752 ± 0.198

4.424 ± 0.717

2.755 ± 0.367

8.645 ± 1.083

5.485 ± 0.841

11.552 ± 0.514

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.282

5.814 ± 0.307

3.969 ± 0.463

4.146 ± 0.263

5.131 ± 0.947

6.775 ± 0.89

5.688 ± 0.447

4.803 ± 0.426

1.365 ± 0.139

3.463 ± 0.438
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski