Lactobacillus phage phiadh

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9T1I4|Q9T1I4_9CAUD Uncharacterized protein OS=Lactobacillus phage phiadh OX=12417 PE=4 SV=1
MM1 pKa = 7.06EE2 pKa = 5.74AKK4 pKa = 9.31TGKK7 pKa = 9.83EE8 pKa = 3.43LLGAINDD15 pKa = 3.82NGNMEE20 pKa = 5.19RR21 pKa = 11.84INRR24 pKa = 11.84LKK26 pKa = 10.47GTDD29 pKa = 4.62SLAVWCGDD37 pKa = 2.99NLYY40 pKa = 10.37IISNNSNVLDD50 pKa = 4.08SDD52 pKa = 3.66FHH54 pKa = 6.21TPAEE58 pKa = 3.94FLNYY62 pKa = 9.52RR63 pKa = 11.84LHH65 pKa = 6.98LLGVSDD71 pKa = 4.9LTHH74 pKa = 6.72EE75 pKa = 4.3MDD77 pKa = 3.17TTEE80 pKa = 5.22FNFFDD85 pKa = 5.1EE86 pKa = 4.83ILDD89 pKa = 4.71AIDD92 pKa = 4.41NGWSQDD98 pKa = 3.55YY99 pKa = 11.14LMEE102 pKa = 5.06ILTDD106 pKa = 4.22CYY108 pKa = 11.2HH109 pKa = 6.57MEE111 pKa = 4.52DD112 pKa = 4.69LSNEE116 pKa = 3.9

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9T1E1|Q9T1E1_9CAUD Uncharacterized protein OS=Lactobacillus phage phiadh OX=12417 PE=4 SV=1
MM1 pKa = 7.25FNKK4 pKa = 10.3AFLAKK9 pKa = 9.96KK10 pKa = 9.87DD11 pKa = 3.53IKK13 pKa = 10.32KK14 pKa = 9.71SQVRR18 pKa = 11.84TMIKK22 pKa = 10.05LKK24 pKa = 10.44RR25 pKa = 11.84YY26 pKa = 8.97LVNLKK31 pKa = 10.36LNNLQVIIAIAMIAIGGSLWYY52 pKa = 9.85DD53 pKa = 2.75RR54 pKa = 11.84HH55 pKa = 6.16YY56 pKa = 11.05FFWPPNLQSALNDD69 pKa = 3.12WRR71 pKa = 11.84IDD73 pKa = 3.39IFILLVGIMLFLATAFRR90 pKa = 11.84PNDD93 pKa = 3.43TLLIRR98 pKa = 11.84TLLVVCGGIVLRR110 pKa = 11.84LGILAIRR117 pKa = 11.84THH119 pKa = 5.72YY120 pKa = 10.84LYY122 pKa = 10.29II123 pKa = 5.47

Molecular weight: 14.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13452

49

1487

213.5

24.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.519 ± 0.35

0.773 ± 0.14

6.735 ± 0.343

5.561 ± 0.417

3.903 ± 0.237

6.527 ± 0.605

1.829 ± 0.177

6.787 ± 0.313

8.631 ± 0.55

7.954 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.138

6.676 ± 0.3

3.137 ± 0.162

4.408 ± 0.273

3.962 ± 0.263

6.69 ± 0.294

6.073 ± 0.271

5.382 ± 0.182

1.762 ± 0.15

4.141 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski